Recombinant Human SREC-I/SCARF1 Fc Chimera Protein, CF

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Binding Activity
Format
Carrier-Free

Order Details

Recombinant Human SREC-I/SCARF1 Fc Chimera Protein, CF Summary

Details of Functionality
Measured by its binding ability in a functional ELISA. Immobilized rhAcLDL at 2 µg/mL (100 µL/well) can bind rhSREC-I/Fc Chimera with a linear range of 0.3-25 ng/mL.
Source
Mouse myeloma cell line, NS0-derived human SREC-I/SCARF1 protein
Human SREC-I
(Ser20 - Thr421)
Accession # Q14162
IEGRMD Human IgG1
(Pro100 - Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Ser20
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
SCARF1
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
69.1 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
100 kDa, reducing conditions
Publications
Read Publications using
2409-SR in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human SREC-I/SCARF1 Fc Chimera Protein, CF

  • Acetyl LDL receptor
  • KIAA0149endothelial cells scavenger receptor
  • MGC47738
  • SCARF1
  • scavenger receptor class F, member 1
  • Scavenger receptor expressed by endothelial cells 1
  • SREC1
  • SRECI
  • SREC-I
  • SRECscavenger receptor expressed by endothelial cells
  • SR-F1

Background

The scavenger receptor (SR) family comprises a group of functionally defined membrane receptors that share a common ability to bind and internalize modified forms of low density lipoproteins (LDL) such as acetylated LDL (AcLDL) and oxidized LDL(OxLDL) (1 - 3). Family members are classified alphabetically. They play important roles in lipid metabolism, in host defence and in the regulation of acquired immunity (2, 4). Scavenger receptor expressed by endothelial cells-I (SREC-I) and SREC-II are two proteins that belong to the F type scavenger receptor group (SR-F1 and SR-F2). The full length cDNA of human SREC-I encodes an 830 amino acid (aa) type I transmembrane protein which contains a 19 aa signal peptide, a 402 aa extracellular region, a 21 aa transmembrane segment, and a 388 aa long cytoplasmic domain. The extracellular region contains ten EGF-like repeats (five of which fit the exact consensus sequence for an EGF-like domain) while the cytoplasmic domain is rich in serine and proline in the N-terminal half, and glycine in the C-terminal segment (5, 6). In addition to the full length form, four SREC-I isoforms exist. Two show insertions of a stop codon in EGF-like domain #8, resulting in mature soluble forms of 323 aa and 318 aa, respectively. A third isoform deletes part of domain #8 plus domains #9 and #10; it continues in-frame to generate a mature transmembrane protein of 725 aa. The last isoform shows only cytoplasmic splicing, with 72 aa substituted for the last 332 aa of the full length form. All three transmembrane forms bind acetylated LDL (6). Native SREC-I is approximately 150 kDa in size, and expressed by endothelial cells, macrophages and fetal neurons (7, 8). In the extracellular region human SREC-I is 76% aa identical to mouse SREC-I. The extracellular regions of human SREC-I and II are 53% aa identical.

  1. Horiuchi, S. et al. (2003) Amino Acids 25:283.
  2. Greaves, D.R. and S. Gordon (2005) J. Lipid Res. 46:11.
  3. Platt, N. and S. Gordon (1998) Chem. Biol. 5:R193.
  4. Platt, N. and S. Gordon (2001) J. Clin. Invest. 108:649.
  5. Adachi, H. et al. (1997) J. Biol. Chem. 272:31217.
  6. Adachi, H. and M. Tsujimoto (2002) J. Biol. Chem. 277:24014.
  7. Shibata, M. et al. (2004) J. Biol. Chem. 279:40084.
  8. Tanura, Y. et al. (2004) J. Biol. Chem. 279:30938.

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Publications for SREC-I/SCARF1 (2409-SR)(5)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 3 applications: Binding Assay, Binding Study, Bioassay.


Filter By Application
Binding Assay
(2)
Binding Study
(1)
Bioassay
(1)
All Applications
Filter By Species
Human
(3)
Mouse
(1)
All Species
Showing Publications 1 - 5 of 5.
Publications using 2409-SR Applications Species
DA Patten, SK Kamarajah, JM Rose, J Tickle, EL Shepherd, DH Adams, CJ Weston, S Shetty SCARF-1 promotes adhesion of CD4+ T cells to human hepatic sinusoidal endothelium under conditions of shear stress Sci Rep, 2017;7(1):17600. 2017 [PMID: 29242513] (Bioassay, Human) Bioassay Human
Biedron R, Konopinski M, Marcinkiewicz J, Jozefowski S Oxidation by neutrophils-derived HOCl increases immunogenicity of proteins by converting them into ligands of several endocytic receptors involved in antigen uptake by dendritic cells and macrophages. PLoS ONE, 2015;10(4):e0123293. 2015 [PMID: 25849867] (Binding Assay, Mouse) Binding Assay Mouse
Baur S, Rautenberg M, Faulstich M, Grau T, Severin Y, Unger C, Hoffmann W, Rudel T, Autenrieth I, Weidenmaier C A nasal epithelial receptor for Staphylococcus aureus WTA governs adhesion to epithelial cells and modulates nasal colonization. PLoS Pathog, 2014;10(5):e1004089. 2014 [PMID: 24788600]
Narazaki M, Segarra M, Hou X, Tanaka T, Li X, Tosato G Oligo-guanosine nucleotide induces neuropilin-1 internalization in endothelial cells and inhibits angiogenesis. Blood, 2010;116(16):3099-107. 2010 [PMID: 20606164] (Binding Assay, Human) Binding Assay Human
Narazaki M, Segarra M, Tosato G Sulfated polysaccharides identified as inducers of neuropilin-1 internalization and functional inhibition of VEGF165 and semaphorin3A. Blood, 2008;111(8):4126-36. 2008 [PMID: 18272814] (Binding Study, Human) Binding Study Human

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Bioinformatics

Gene Symbol SCARF1
Uniprot