Recombinant Human Sonic Hedgehog/Shh, N-Terminus Protein

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1 μg/lane of Recombinant Human Sonic Hedgehog/Shh, N-Terminus was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 22 kDa.
Recombinant Human Sonic Hedgehog/Shh, N-Terminus (Catalog # 1314-SH) induces alkaline phosphatase production by the C3H10T1/2 mouse embryonic fibroblast cell line. The ED50 for this effect is <5 μg/mL.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity

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Catalog# & Formulation Size Price

Recombinant Human Sonic Hedgehog/Shh, N-Terminus Protein Summary

Additional Information
A New rh Shh is Available! C-term cholesterol, N-term fatty acid-modified; 250-fold better activity!
Details of Functionality
Measured by its ability to induce alkaline phosphatase production by C3H10T1/2 mouse embryonic fibroblast cells. Nakamura, T. et al. (1997) Biochem. Biophys. Res. Commun. 237:465. The ED50 for this effect is <5 µg/mL.
Source
E. coli-derived human Sonic Hedgehog/Shh protein
Cys24-Gly197, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Cys24
Protein/Peptide Type
Recombinant Proteins
Gene
SHH
Purity
>97%, by SDS-PAGE with silver staining, under reducing conditions
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
20 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
22 kDa, reducing conditions
Publications
Read Publications using
1314-SH in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in NaH2PO4, NaCl and DTT with BSA as a carrier protein.
Purity
>97%, by SDS-PAGE with silver staining, under reducing conditions
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Sonic Hedgehog/Shh, N-Terminus Protein

  • HHG1
  • HHG-1
  • HLP3
  • HPE3
  • MCOPCB5
  • MCOPCB5sonic hedgehog (Drosophila) homolog
  • Shh
  • ShhNC
  • SMMCI
  • SMMCIsonic hedgehog homolog (Drosophila)
  • sonic hedgehog homolog
  • sonic hedgehog protein
  • Sonic Hedgehog
  • TPT
  • TPTPS

Background

Sonic Hedgehog (Shh) is expressed in embryonic tissues that are critical for the patterning of the developing central nervous system, somite, and limb. It is also involved in whisker, hair, foregut, tooth, and bone development. Shh regulates neural and hematopoietic stem cell fate and is important for thymocyte differentiation and proliferation as well as T cell determination. In adult tissue Shh is associated with cancer development and tissue remodeling following injury (1-3). Human Shh encodes a 462 amino acid (aa) precursor protein that is autocatalytically processed to yield a non-glycosylated 19 kDa N-terminal fragment (Shh-N) and a glycosylated 25 kDa C-terminal protein (Shh-C) (4). Shh-C, which is responsible for the intramolecular processing of Shh, is rapidly degraded following Shh proteolysis (5). Shh-N is highly conserved, sharing >98% aa identity between mouse, human, rat, canine, porcine, and chicken Shh-N. Shh-N can be palmitoylated at its
N-terminal cysteine and modified by cholesterol addition at its C-terminus (6). These modifications contribute to the membrane tethering of Shh as well as its assembly into various sized multimers (6-9). Lipid modification and multimerization greatly increase Shh-N receptor binding affinity and signaling potency (5, 6, 8, 9). Monomeric and multimeric Shh can be released from the plasma membrane by the cooperative action of DISP1, SCUBE2, and TACE/ADAM17 (10-12). Modifications also extend the effective range of Shh functionality and are required for the development of protein gradients important in tissue morphogenesis (9, 13). Canonical signaling of Shh is mediated by a multicomponent receptor complex that includes Patched (PTCH1, PTCH2) and Smoothened (SMO) (14). The binding of Shh to PTCH releases the basal repression of SMO by PTCH. Shh activity can also be regulated through interactions with heparin, glypicans, and membrane-associated Hip (hedgehog interacting protein) (13, 15, 16).
  1. Briscoe, J. and P.P. Therond (2013) Mol. Cell. Biol. 14:416.
  2. Aviles, E.C. et al. (2013) Front. Cell. Neurosci. 7:86.
  3. Xie, J. et al. (2013) OncoTargets Ther. 6:1425.
  4. Marigo, V. et al. (1995) Genomics 28:44.
  5. Zeng, X. et al. (2001) Nature 411:716.
  6. Feng, J. et al. (2004) Development 131:4357.
  7. Goetz, J.A. et al. (2006) J. Biol. Chem. 281:4087.
  8. Pepinsky, R.B. et al. (1998) J. Biol. Chem. 273:14037.
  9. Chen, M.-H. et al. (2004) Genes Dev. 18:641.
  10. Etheridge, L.A. et al. (2010) Development 137:133.
  11. Jakobs, P. et al. (2014) J. Cell Sci. 127:1726.
  12. Dierker, T. et al. (2009) J. Biol. Chem. 284:8013.
  13. Lewis, P.M. et al. (2001) Cell 105:599.
  14. Carpenter, D. et al. (1998) Proc. Natl. Acad. Sci. USA 95:13630.
  15. Filmus, J. and M. Capurro (2014) Matrix Biol. 35:248.
  16. Chuang, P.-T. and A.P. McMahon (1999) Nature 397:617.

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1314-SH
Species: Hu
Applications: Bioactivity

Publications for Sonic Hedgehog/Shh (1314-SH)(10)

We have publications tested in 5 confirmed species: Human, Mouse, Bovine, Chicken, N/A.

We have publications tested in 3 applications: Bioassay, Cell Culture, SDS-PAGE.


Filter By Application
Bioassay
(8)
Cell Culture
(1)
SDS-PAGE
(1)
All Applications
Filter By Species
Human
(5)
Mouse
(2)
Bovine
(1)
Chicken
(1)
N/A
(1)
All Species
Showing Publications 1 - 10 of 10.
Publications using 1314-SH Applications Species
Y Arribat, KS Mysiak, L Lescouzère, A Boizot, M Ruiz, M Rossel, P Bomont Sonic Hedgehog repression underlies gigaxonin mutation-induced motor deficits in giant axonal neuropathy J. Clin. Invest., 2019;0(0):. 2019 [PMID: 31503551] (Cell Culture, Mouse) Cell Culture Mouse
T Johansen, C Krabbe, SI Schmidt, AM Serrano, M Meyer Comparative Analysis of Spontaneous and Stimulus-Evoked Calcium Transients in Proliferating and Differentiating Human Midbrain-Derived Stem Cells Stem Cells Int, 2017;2017(0):9605432. 2017 [PMID: 29201062] (Bioassay, Human) Bioassay Human
E El Agha, V Kheirollah, A Moiseenko, W Seeger, S Bellusci Ex vivo analysis of the contribution of FGF10(+) cells to airway smooth muscle cell formation during early lung development Dev. Dyn., 2017;0(0):. 2017 [PMID: 28387977] (Bioassay, Mouse) Bioassay Mouse
Xu Y, An Y, Wang X, Zha W, Li X Inhibition of the Hedgehog pathway induces autophagy in pancreatic ductal adenocarcinoma cells. Oncol Rep, 2014;31(2):707-12. 2014 [PMID: 24297612] (Bioassay, Human) Bioassay Human
Aad, Pauline, Echternkamp, Sherrill, Sypherd, David D, Schreiber, Nicole B, Spicer, Leon J The hedgehog system in ovarian follicles of cattle selected for twin ovulations and births: evidence of a link between the IGF and hedgehog systems. Biol Reprod, 2012;87(4):79. 2012 [PMID: 22811575] (Bioassay, Bovine) Bioassay Bovine
Erceg S, Lukovic D, Moreno-Manzano V, Stojkovic M, Bhattacharya S Derivation of cerebellar neurons from human pluripotent stem cells. Curr Protoc Stem Cell Biol, 2012;20(0):1H.51. 2012 [PMID: 22415839] (Bioassay, Human) Bioassay Human
Gil JE, Woo DH, Shim JH, Kim SE, You HJ, Park SH, Paek SH, Kim SK, Kim JH Vitronectin promotes oligodendrocyte differentiation during neurogenesis of human embryonic stem cells. FEBS Lett., 2009;583(3):561-7. 2009 [PMID: 19162023] (Bioassay, Human) Bioassay Human
Elkabetz Y, Panagiotakos G, Al Shamy G, Socci ND, Tabar V, Studer L Human ES cell-derived neural rosettes reveal a functionally distinct early neural stem cell stage. Genes Dev., 2008;22(2):152-65. 2008 [PMID: 18198334] (Bioassay, Human) Bioassay Human
Zavros Y, Waghray M, Tessier A, Bai L, Todisco A, L Gumucio D, Samuelson LC, Dlugosz A, Merchant JL Reduced pepsin A processing of sonic hedgehog in parietal cells precedes gastric atrophy and transformation. J. Biol. Chem., 2007;282(46):33265-74. 2007 [PMID: 17872943] (SDS-PAGE, N/A) SDS-PAGE N/A
Nordstrom U, Maier E, Jessell TM, Edlund T An early role for WNT signaling in specifying neural patterns of Cdx and Hox gene expression and motor neuron subtype identity. PLoS Biol., 2006;4(8):e252. 2006 [PMID: 16895440] (Bioassay, Chicken) Bioassay Chicken

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Gene Symbol SHH
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