Recombinant Human Siglec-8 Fc Chimera Protein, CF

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human Siglec-8 Fc Chimera Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. When Biotinylated Sialyl Lewis X was immobilized at 0.05 μg/mL (100 μL/well), it binds Recombinant Human Siglec-8 Fc Chimera. The concentration of Human Siglec-8 Fc Chimera that produces 50% of the optimal binding response is approximately 0.2-1.2 μg/mL.
• Source: Mouse myeloma cell line, NS0-derived human Siglec-8 protein
  

  Human Siglec-8 (Met17-Ala363) Accession # AAH53319	IEGRMD	Human IgG1 (Pro100-Lys330)
  N-terminus		C-terminus

• Accession #: AAH53319
• N-terminal Sequence: Met17
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Gene: SIGLEC8
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 64 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 68-88 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Siglec-8 Fc Chimera Protein, CF

• CD329 antigen
• CDw329
• MGC59785
• SAF2
• SAF2SAF-2
• sialic acid binding Ig-like lectin 8
• Sialoadhesin family member 2
• Siglec8
• Siglec-8
• SIGLEC8L
• SIGLEC8Lsialic acid-binding Ig-like lectin 8

Background

Siglec-8, also known as SAF, is an approximately 75 kDa transmembrane glycoprotein in the Siglec family of sialic acid-binding immune regulatory molecules (1). Mature human Siglec-8 consists of a 347 amino acid (aa) extracellular domain (ECD) with three Ig-like domains, a 21 aa transmembrane segment, and a 115 aa cytoplasmic domain with two tyrosine based signaling motifs (2, 3). Alternative splicing generates additional isoforms that either lack most of the second Ig-like domain or have a substituted cytoplasmic domain without the signaling motifs (4, 5). Siglec-8 is expressed on eosinophils, basophils, and mast cells (2, 3), and it shows a binding preference for the carbohydrate 6-O sulfated sLe^x (3, 6, 7). In mouse, Siglec-F is a functional paralog of human Siglec-8, as shown by its similar expression profile and ligand specificity (8). Cross-linking of Siglec-8 inhibits Fc epsilon RI alpha induced mast cell degranulation (9). It also induces eosinophil apoptosis, an effect which is enhanced by the eosinophil-activating cytokines IL-5, IL-33, and GM-CSF (10-12).

1. Kiwamoto, T. et al. (2013) Curr. Opin. Allergy Clin. Immunol. 13:106.
2. Kikly, K.K. et al. (2000) J. Allergy Clin. Immunol. 105:1093.
3. Floyd, H. et al. (2000) J. Biol. Chem. 275:861.
4. Aizawa, H. et al. (2002) J. Allergy Clin. Immunol. 109:176.
5. Foussias, G. et al. (2000) Biochem. Biophys. Res. Commun. 278:775.
6. Bochner, B.S. et al. (2005) J. Biol. Chem. 280:4307.
7. Hudson, S.A. et al. (2011) J. Clin. Immunol. 31:1045.
8. Tateno, H. et al. (2005) Glycobiology 15:1125.
9. Yokoi, H. et al. (2008) J. Allergy Clin. Immunol. 121:499.
10. Kano, G. et al. (2013) J. Allergy Clin. Immunol. 132:437.
11. Nutku, E. et al. (2003) Blood 101:5014.
12. Na, H.J. et al. (2012) Cytokine 57:169.

Publications for Siglec-8 (9045-SL)(1)

Publication using 9045-SL

• Park, C;Hwang, IY;Yan, SL;Vimonpatranon, S;Wei, D;Van Ryk, D;Girard, A;Cicala, C;Arthos, J;Kehrl, JH; Murine alveolar macrophages rapidly accumulate intranasally administered SARS-CoV-2 Spike protein leading to neutrophil recruitment and damage eLife 2024-03-20 [PMID: 38507462] (Bioassay, Human)

Bioinformatics

• Gene Symbol: SIGLEC8
• Uniprot:
  • AAH53319()