Recombinant Human SHBG Protein, CF

• Reactivity: Hu
• Format: Carrier-Free

Recombinant Human SHBG Protein, CF Summary

• Details of Functionality: Bioassay data are not available.
• Source: Mouse myeloma cell line, NS0-derived human SHBG protein Leu30-His402, with a C-terminal 6-His tag
• Accession #: P04278-1
• N-terminal Sequence: Leu30
• Protein/Peptide Type: Innovator Recombinant Proteins
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity not tested
• Theoretical MW: 41 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 41-52 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 3 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining
• Reconstitution Instructions: Reconstitute at 250 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human SHBG Protein, CF

• ABP
• ABPTestosterone-estrogen-binding globulin
• androgen binding protein
• MGC126834
• MGC138391
• SBP
• sex hormone-binding globulin
• Sex steroid-binding protein
• SHBG
• TEBG
• Testis-specific androgen-binding protein
• testosterone-binding beta-globulin
• Testosterone-estradiol-binding globulin

Background

Human SHBG (Sex hormone-binding globulin), also known as ABP (Testis-specific Androgen-binding protein) or TeBG (Testosterone-estradiol-binding globulin), is a variably glycosylated, secreted non-disulfide linked homodimer that belongs to the SHBG family (1). Members of this small family have tandem repeats of 170 amino acid (aa) long laminin alpha chain G- like domains. Each SHBG monomer is a 47-53 kDa, 373 aa glycoprotein that contains one steroid-binding site in its N-terminal G- like domain (2). SHBG is synthesized by the liver and circulates in blood. It binds to certain androgens and estrogens and serves as a transporter for the bound steroids (3); it is also involved in receptor mediated processes (4). Human SHBG shares approximately 67% aa sequence identity with mouse and rat SHBG. Low SHGB levels are associated with increased risk for type 2 diabetes for both man and women (5).

1. Awakumov G.V. et al. (2010) Mol. Cell Endocrinol. 5:316.
2. Grishkovskaya I. et al. (2000) EMBO J. 19:504.
3. Anderson D.C. (1974) Clin. Endocrinol. (Oxf.) 3:69.
4. Rosner W. et al. (2010) Mol. Cell Endocrinol.5:795.
5. Muka T. et al. (2017) Diabetes. 66:577.

Bioinformatics

• Uniprot:
  • P04278-1()