Recombinant Human Semaphorin 6A Fc Chimera Protein, CF

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

Recombinant Human Semaphorin 6A Fc Chimera Protein, CF Summary

Details of Functionality
Measured by its ability to inhibit the proliferation of HUVEC human umbilical vein endothelial cells. Moriya, J. et al. (2010) Circ. Res. 106:391. The ED50 for this effect is 0.4-2.4 μg/mL.
Source
Mouse myeloma cell line, NS0-derived human Semaphorin 6A protein
Human Semaphorin 6A
(Gly19-Thr649)
Accession # Q9H2E6
DIEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Gly19
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
SEMA6A
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
97 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
120 kDa, reducing conditions
Publications
Read Publications using
1146-S6 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Semaphorin 6A Fc Chimera Protein, CF

  • KIAA1368HT018
  • sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6A
  • Sema VIA
  • SEMA
  • Sema6A
  • SEMA6A1
  • SEMA6A-1
  • Semaphorin 6A
  • semaphorin 6A-1
  • Semaphorin VIA
  • semaphorin-6A
  • semaphorin-6A-1
  • SEMAQ
  • VIA

Background

Semaphorin 6A (Sema6A) is an approximately 120 kDa member of the class 6 subfamily of semaphorins, a large, highly conserved family of signaling molecules that affect multiple processes including axon guidance, cell migration, synaptogenesis, dendritic spine formation, and angiogenesis (1). The class 6 semaphorins are type I transmembrane glycoproteins that contain a characteristic extracellular beta  propeller N-terminal semaphorin (sema) domain and also an extracellular
plexin-semaphorin-integrin (PSI) domain (1, 2). Within the ECD, human Sema6A shares 94% and 93% aa sequence identity with mouse and rat Sema6A, respectively. Alternative splicing generates an additional isoform with a 17 aa insertion between the sema domain and transmembrane segment. Sema6A interacts with Plexin A4
(3-7) to induce growth cone collapse and regulate the axon pathfinding of sympathetic neurons (3, 7, 8), cerebellar cortex granule cells (9), hippocampus CA3 region mossy fibers (6), corticospinal tract axons (5, 10), and retinal neurons (4). Plexin A2 can compete with Plexin A4 for Sema6A binding and thereby limit axon repulsion (6). In addition, Sema6A and Plexin A4 are co-expressed on dorsal root ganglion sensory neurons and associate in cis; this prevents in trans interactions and subsequent axon repulsion (7). Sema6A is additionally expressed on vascular endothelial cells where it regulates VEGF R2 responsiveness during angiogenesis (11). In contrast, exogenous Sema6A can inhibit the survival of the HUVEC cell line in a Plexin A4 independent manner (11, 12). Sema6A is also expressed on myelinating oligodendrocytes, Langerhans cells, and some dendritic cells (13, 14).
  1. Jongbloets, B.C. and R.J. Pasterkamp (2014) Development 141:3292.
  2. Kikuchi, K. et al. (1999) Mol. Cell. Neurosci. 13:9.
  3. Suto, F. et al. (2005) J. Neurosci. 25:3628.
  4. Matsuoka, R.L. et al. (2011) Nature 470:259.
  5. Runker, A.E. et al. (2008) Neural Dev. 3:34.
  6. Suto, F. et al. (2007) Neuron 53:535.
  7. Haklai-Topper, L. et al. (2010) EMBO J. 29:2635.
  8. Xu, X.M. et al. (2000) J. Neurosci. 20:2638.
  9. Kerjan, G. et al. (2005) Nat. Neurosci. 8:1516.
  10. Mauti, O. et al. (2007) Neural Dev. 2:28.
  11. Segarra, M. et al. (2012) Blood 120:4104.
  12. Kigel, B. et al. (2011) Blood 118:4285.
  13. Bernard, F. et al. (2012) Glia 60:1590.
  14. Gautier, G. et al. (2006) Am. J. Pathol. 168:453.

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Publications for Semaphorin 6A (1146-S6)(5)

We have publications tested in 2 confirmed species: Human, Zebrafish.

We have publications tested in 1 application: Bioassay.


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(5)
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(4)
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(1)
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Bioinformatics

Gene Symbol SEMA6A
Entrez
Uniprot