Recombinant Human PTPR sigma Protein, CF

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human PTPR sigma Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. When Recombinant Human PTPR sigma /PTPRS is coated at 2 μg/mL, Recombinant Human TrkC Fc Chimera (Catalog # 373‑TC) binds with an apparent K_d <1 nM.
• Source: Mouse myeloma cell line, NS0-derived human PTP sigma/PTPRS protein
  Glu30-Gly1260, with a C-terminal 6-His tag
• Accession #: NP_570924
• N-terminal Sequence: Glu30 & Ser1173
• Protein/Peptide Type: Recombinant Proteins
• Gene: PTPRS
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 135.8 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 120-145 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in HEPES and NaCl.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human PTPR sigma Protein, CF

• EC 3.1.3.48
• protein tyrosine phosphatase PTPsigma
• protein tyrosine phosphatase, receptor type, S
• protein tyrosine phosphatase, receptor type, sigma
• PTP sigma
• PTPR sigma
• PTPRS
• PTPSIGMA
• receptor-type tyrosine-protein phosphatase S
• Receptor-type tyrosine-protein phosphatase sigma
• R-PTP-S
• R-PTP-sigma

Background

Phosphatase Receptor-type sigma (PTPR sigma ), also known as PTP sigma , is a 200 kDa type I transmembrane glycoprotein that is a member of the class 2A (LAR) subfamily of the protein tyrosine phosphatase (PTP) family (1). Human PTPR sigma cDNA encodes 1948 amino acids (aa) including a 29 aa signal sequence, a 1253 aa extracellular domain (ECD) with three C2-type Ig domains and eight Fibronectin (Fn) type III domains, a transmembrane domain, and a cytoplasmic region with two PTP domains (1, 2). Multiple differentially expressed splice variants lack sections of the Ig and/or Fn domains and/or cytoplasmic juxtamembrane sequence (1, 2). PKC activation in the cell promotes endoprotease cleavage of PTPR sigma , releasing the 140-160 kDa ECD (3). The human PTPR sigma ECD shares 93% aa sequence identity with the nearest isoform of mouse and rat PTPR sigma , 96% with bovine PTPR sigma , and 97% with porcine PTPR sigma . Activity of PTPR sigma can be inhibited by bisphosphonates (2). PTPR sigma is a receptor for several proteins, including neural chondroitin sulfate proteoglycans which inhibit nerve regeneration (4, 5). It also binds and dephosphorylates targets such as N-Cadherin, E-Cadherin, EGF R and Ret (6-9). Through these interactions PTPR sigma affects pituitary and neuroendocrine development, and axon growth and targeting (4-6). Mice deleted for PTPR sigma show pituitary, pancreatic, and gut abnormalities, mainly due to growth hormone insufficiency (9-12). They also show enhanced regeneration, but faulty targeting, of injured peripheral nerves (4-6, 13). Head and neck squamous cell cancers frequently underexpress PTPR sigma (14). Since PTPR sigma inhibits EGF R signaling pathways, its underexpression can enhance tumor cell growth (14).

1. Pulido, R. et al. (1995) Proc. Natl. Acad. Sci. USA 92:11686.
2. Endo, N. et al. (1996) J. Bone Miner. Res. 11:535.
3. Aicher, B. et al. (1997) J. Cell Biol. 138:681.
4. Shen, Y. et al. (2009) Science 326:592.
5. Duan, Y. and R.J. Giger (2010) Sci. Signal. 3:pe6.
6. Siu, R. et al. (2007) Mol. Cell. Biol. 27:208.
7. Vijayvargia, R. et al. (2004) Biochem. Biophys. Res. Commun. 325:344.
8. Uetani, N. et al. (2009) J. Clin. Invest. 119:924.
9. Muise, A.M. et al. (2007) Curr. Biol. 17:1212.
10. Elchebly, M. et al. (1999) Nat. Genet. 21:330.
11. Batt, J. et al. (2002) Mol. Endocrinol. 16:155.
12. Chagnon, M.J. et al. (2006) Can. J. Physiol. Pharmacol. 84:755.
13. McLean, J. et al. (2002) J. Neurosci. 22:5481.
14. Morris, L.G. et al. (2011) Proc. Natl. Acad. Sci. USA 108:19024.

Bioinformatics

• Gene Symbol: PTPRS
• Uniprot:
  • NP_570924()