Recombinant Human Podocalyxin Protein, CF

Immobilized Recombinant Human Podocalyxin inhibits the adhesion of CHO Chinese hamster ovary cells transfected with P-Selectin.

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human Podocalyxin Protein, CF Summary

• Details of Functionality: Measured by the ability of the immobilized protein to inhibit the adhesion of CHO Chinese hamster ovary cells transfected with P-Selectin. The ED₅₀ for this effect is 0.08-0.4 μg/mL.
• Source: Mouse myeloma cell line, NS0-derived human Podocalyxin protein
  Ser23-Arg427 and a C-terminal Asp and 10-His tag
• Accession #: AAB61574.1
• N-terminal Sequence: Ser23
• Structure / Form: Monomer
• Protein/Peptide Type: Recombinant Proteins
• Gene: PODXL
• Purity: >95%, by SDS-PAGE with silver staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 43 kDa (monomer).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 125-165 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE with silver staining.
• Reconstitution Instructions: Reconstitute at 100 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Podocalyxin Protein, CF

• GCTM
• Gp200MGC138240
• PCLP
• PCLP1
• PCLP-1
• PCLPGCTM-2 antigen
• PCpodocalyxin
• POD XL
• Podocalyxin
• Podocalyxin-like protein 1
• podocalyxin-like
• PODXL

Background

Podocalyxin (PODXL), also known as Podocalyxin-like protein 1 (PCLP-1), is a heavily glycosylated transmembrane sialoprotein in the CD34 and Endoglycan family (1). Mature human Podocalyxin consists of a 406 amino acid (aa) extracellular domain (ECD), a 26 aa transmembrane segment, and a 75 aa cytoplasmic domain (2). Within the ECD, human Podocalyxin shares 31% aa sequence identity with mouse and rat Podocalyxin. Alternative splicing of human Podocalyxin generates an additional isoform with a 33 aa deletion in the ECD. Podocalyxin is expressed on glomerular and vascular endothelial cells (2, 3), neurons (4), hematopoietic, mesenchymal, and cardiac stem cells (5-8), and metastatic carcinoma and glioblastoma tumor cells (9-11). A soluble form of Podocalyxin can be released into the urine of women with pre-eclampsia (12). Podocalyxin promotes platelet activation (13), neurite outgrowth, branching, and synaptogenesis (4), homing of hematopoietic progenitor cells to the bone marrow and spleen (6), and engraftment of mesenchymal and cardiac stem cells into ischemic heart and kidney (7, 8). Its interactions with L-Selectin and E-Selectin mediate the tethering of lymphocytes and metastatic tumor cells to the vascular endothelium (3, 9, 10). Podocalyxin can be expressed as a 160-165 kDa molecule or as even larger glycoforms (2, 3, 10, 14). In humans, an approximately 200 kDa glycoform contains the carbohydrate antigen recognized by TRA-1-60 and TRA-1-81 antibodies used for the identification of undifferentiated pluripotent embryonic stem cells (14).

1. Nielsen, J.S. and K.M. McNagny (2009) J. Am. Soc. Nephrol. 20:1669.
2. Kershaw, D.B. et al. (1997) J. Biol. Chem. 272:15708.
3. Sassetti, C. et al. (1998) J. Exp. Med. 187:1965.
4. Vitureira, N. et al. (2010) PLoS ONE 8:e12003.
5. Zhang, H. et al. (2014) Stem Cells 32:191.
6. Doyonnas, R. et al. (2005) Blood 105:4170.
7. Lee, R-H. et al. (2009) Blood 113:816.
8. Moscoso, I. et al. (2013) J. Tissue Eng. Regen. Med. Epub. PMID 23897803.
9. Dallas, M.R. et al. (2012) Am. J. Physiol. Cell Physiol. 303:C616.
10. Thomas, S.N. et al. (2009) Am. J. Cell Physiol. 296:C505.
11. Binder, Z.A. et al. (2013) PLoS ONE 8:e75945.
12. Wang, Y. et al. (2012) Am. J. Physiol. Renal Physiol. 302:F1084.
13. Alonso-Martin, S. et al. (2010) Thromb. Res. 125:e300.
14. Schopperle, W.M. and W.C. DeWolf (2007) Stem Cells 25:723.

Publications for Podocalyxin Like (1658-PD)(4)

Publications using 1658-PD

• Chongsaritsinsuk, J;Rangel-Angarita, V;Mahoney, KE;Lucas, TM;Enny, OM;Katemauswa, M;Malaker, SA; Quantification and site-specific analysis of co-occupied N- and O-glycopeptides bioRxiv : the preprint server for biology 2024-07-07 [PMID: 39005468] (Bioassay, N/A)
• BJ Medley, L Leclaire, N Thompson, KE Mahoney, B Pluvinage, MAH Parson, JE Burke, S Malaker, W Wakarchuk, AB Boraston A previously uncharacterized O-glycopeptidase from Akkermansia muciniphila requires the Tn-antigen for cleavage of the peptide bond The Journal of Biological Chemistry, 2022-08-30;0(0):102439. 2022-08-30 [PMID: 36049519] (Bioassay, Human)
• DJ Shon, D Fernandez, NM Riley, MJ Ferracane, CR Bertozzi Structure-guided mutagenesis of a mucin-selective metalloprotease from Akkermansia muciniphila alters substrate preferences The Journal of Biological Chemistry, 2022-04-09;0(0):101917. 2022-04-09 [PMID: 35405095] (Bioassay, Akkermansia muciniphila)
• SA Malaker, K Pedram, MJ Ferracane, BA Bensing, V Krishnan, C Pett, J Yu, EC Woods, JR Kramer, U Westerlind, O Dorigo, CR Bertozzi The mucin-selective protease StcE enables molecular and functional analysis of human cancer-associated mucins Proc. Natl. Acad. Sci. U.S.A., 2019-03-25;0(0):. 2019-03-25 [PMID: 30910957] (Bioassay, Bacteria - E. Coli)

Bioinformatics

• Gene Symbol: PODXL
• Uniprot:
  • AAB61574.1()