Recombinant Human PDGF R beta Fc Chimera Protein, CF

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

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Catalog# & Formulation Size Price

Recombinant Human PDGF R beta Fc Chimera Protein, CF Summary

Details of Functionality
Measured by its ability to inhibit the biological activity of PDGF-BB using NR6R‑3T3 mouse fibroblast cells. Raines, E.W. et al. (1985) Methods Enzymol. 109:749. The ED50 for this effect is 1-3 µg/mL in the presence of 50 ng/mL Recombinant Human PDGF‑BB (Catalog # 220-BB).
Source
Mouse myeloma cell line, NS0-derived human PDGF R beta protein
Human PDGF R beta
Leu33-Phe530
(Glu241Asp)
Accession # P09619
ENIEGRMD Human IgG1
(Pro100-Lys330)
6-His tag
N-terminus C-terminus
Accession #
N-terminal Sequence
Leu33
Protein/Peptide Type
Recombinant Proteins
Gene
PDGFRB
Purity
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
84 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
150 kDa, reducing conditions
Publications
Read Publications using
385-PR/CF in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 500 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human PDGF R beta Fc Chimera Protein, CF

  • beta-type platelet-derived growth factor receptor
  • CD140 antigen-like family member B
  • CD140b antigen
  • CD140b
  • EC 2.7.10
  • EC 2.7.10.1
  • JTK12
  • PDGF R beta
  • PDGFR
  • PDGFR1
  • PDGFRB
  • PDGF-R-beta
  • platelet-derived growth factor receptor, beta polypeptide

Background

PDGF is a major serum mitogen that can exist as a homo or hetero-dimeric protein consisting of disulfide-linked PDGF-A and PDGF-B chains. The PDGF-AA,
PDGF-BB and PDGF-AB isoforms have been shown to bind to two distinct cell surface PDGF receptors with different affinities. Where as PDGF R alpha binds all three PDGF isoforms with high affinity, PDGF R beta binds PDGF-BB only with high-affinity. Both PDGF R alpha and PDGF R beta  are members of the class III subfamily of receptor tyrosine kinases (RTK) that also includes the receptors for M-CSF, SCF and Flt3 ligand. All class III RTKs are characterized by the presence of
five immunoglobulin-like domains in their extracellular region and a split kinase domain in their intracellular region. PDGF binding induces receptor homo-and
hetero-dimerization and signal transduction. The expression of the alpha and beta receptors is independently regulated in various cell types. Recombinant soluble PDGF R beta binds PDGF with high affinity and is potent PDGF antagonist.

  1. Heldin, C.H. and L. Claesson-Welsh (1994) in Guidebook to Cytokines and Their Receptors, Nicola, N.A. ed. Oxford University Press, New York, p. 202.

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Publications for PDGF R beta (385-PR/CF)(10)

We have publications tested in 3 confirmed species: Human, Rat, N/A.

We have publications tested in 6 applications: Bioassay, ELISA Standard, ELISA capture, In Vivo, Surface Plasmon Resonance, TaqMan Protein Assay Ctrl.


Filter By Application
Bioassay
(5)
ELISA Standard
(1)
ELISA capture
(1)
In Vivo
(1)
Surface Plasmon Resonance
(1)
TaqMan Protein Assay Ctrl
(1)
All Applications
Filter By Species
Human
(6)
Rat
(1)
N/A
(2)
All Species
Showing Publications 1 - 10 of 10.
Publications using 385-PR/CF Applications Species
SB Mamer, S Chen, JC Weddell, A Palasz, A Wittenkell, M Kumar, PI Imoukhuede Discovery of High-Affinity PDGF-VEGFR Interactions: Redefining RTK Dynamics Sci Rep, 2017;7(1):16439. 2017 [PMID: 29180757] (Surface Plasmon Resonance, Human) Surface Plasmon Resonance Human
Z Tao, H Yang, Q Shi, Q Fan, L Wan, X Lu Targeted Delivery to Tumor-associated Pericytes via an Affibody with High Affinity for PDGFR? Enhances the in vivo Antitumor Effects of Human TRAIL Theranostics, 2017;7(8):2261-2276. 2017 [PMID: 28740549] (Bioassay, N/A) Bioassay N/A
C Stegmann, D Hochdorfer, D Lieber, N Subramania, D Stöhr, K Laib Sampa, C Sinzger A derivative of platelet-derived growth factor receptor alpha binds to the trimer of human cytomegalovirus and inhibits entry into fibroblasts and endothelial cells PLoS Pathog., 2017;13(4):e1006273. 2017 [PMID: 28403220] (Bioassay, Human) Bioassay Human
Y Wu, A Prager, S Boos, M Resch, I Brizic, M Mach, S Wildner, L Scrivano, B Adler Human cytomegalovirus glycoprotein complex gH/gL/gO uses PDGFR-? as a key for entry PLoS Pathog., 2017;13(4):e1006281. 2017 [PMID: 28403202] (Bioassay, Human) Bioassay Human
S Lykkemark, OA Mandrup, MB Jensen, J Just, P Kristensen A novel excision selection method for isolation of antibodies binding antigens expressed specifically by rare cells in tissue sections Nucleic Acids Res., 2017;0(0):. 2017 [PMID: 28369551] (ELISA Standard, Human) ELISA Standard Human
Kun Ding Generation and characterization of ABBV642, a dual variable domain immunoglobulin molecule (DVD-Ig) that potently neutralizes VEGF and PDGF-BB and is designed for the treatment of exudative age-related macular degeneration MAbs, 2016;0(0):0. 2016 [PMID: 27929753] (Bioassay) Bioassay
Donica C, Cui Y, Shi S, Gutstein H Platelet-derived growth factor receptor-beta antagonism restores morphine analgesic potency against neuropathic pain. PLoS ONE, 2014;9(5):e97105. 2014 [PMID: 24820332] (In Vivo, Rat) In Vivo Rat
Camorani S, Esposito C, Rienzo A, Catuogno S, Iaboni M, Condorelli G, de Franciscis V, Cerchia L Inhibition of receptor signaling and of glioblastoma-derived tumor growth by a novel PDGFRbeta aptamer. Mol Ther, 2014;22(4):828-41. 2014 [PMID: 24566984] (Bioassay, Human) Bioassay Human
Vascular endothelial growth factor signals through platelet-derived growth factor receptor beta in meningiomas in vitro. Br. J. Cancer, 2012;107(10):1702-13. 2012 [PMID: 23047550] (TaqMan Protein Assay Ctrl, N/A) TaqMan Protein Assay Ctrl N/A
Lacoste E, Martineau I, Gagnon G Platelet concentrates: effects of calcium and thrombin on endothelial cell proliferation and growth factor release. J. Periodontol., 2003;74(10):1498-507. 2003 [PMID: 14653397] (ELISA capture, Human) ELISA capture Human

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Bioinformatics

Gene Symbol PDGFRB
Entrez
Uniprot