Recombinant Human P-Selectin/CD62P Protein, CF

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human P-Selectin/CD62P Protein, CF Summary

• Details of Functionality: Measured by the ability of the immobilized protein to support the adhesion of U937 human histiocytic lymphoma cells. When 5 x 10⁴ cells/well are added to human sP-Selectin coated plates (10 μg/mL with 100 μL/well), >50% will adhere after 1 hour incubation at room temperature.
• Source: Chinese Hamster Ovary cell line, CHO-derived human P-Selectin/CD62P protein
  Trp42-Ala771
• Accession #: P16109
• N-terminal Sequence: Trp42
• Protein/Peptide Type: Recombinant Proteins
• Gene: SELP
• Purity: >97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 80 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 121-124 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS containing Calcium and Magnesium with Sorbitol.
• Purity: >97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 1 mg/mL in sterile, deionized water.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human P-Selectin/CD62P Protein, CF

• CD62P antigen
• CD62P
• FLJ45155
• GMP140
• GRMP
• PADGEM
• PADGEMantigen CD62)
• PSEL
• P-Selectin
• selectin P (granule membrane protein 140kDa, antigen CD62)
• SELP

Background

Human P-Selectin (GMP-140, LECAM-3, PADGEM, CD62P), a member of the Selectin family, is a cell surface glycoprotein expressed by activated platelets and endothelial cells. P-Selectin is translocated to the cell surface within minutes, from alpha granules of platelets or Weibel-Palade bodies of endothelial cells, following stimulation with thrombin, histamine, PMA or peroxides. P-Selectin binds to a 106 kDa protein present on myeloid cells, neutrophils, monocytes and lymphocytes, termed PSGL-1 (P-Selectin glycoprotein ligand-1).

P-Selectin plays a role in the adhesion of leukocytes and neutrophils to the endothelium. Acting in cooperation with L Selectin,P-Selectin mediates the initial interaction of circulating leukocytes with endothelial cells that produces a characteristic ‘rolling' of the leukocytes on the endothelium. This initial interaction is followed by a stronger interaction involving E-Selectin, and later ICAM-1 and VCAM-1, that leads eventually to extravasation of the white blood cell through the blood vessel wall into the extracellular matrix tissue.

ELISA techniques have shown that detectable levels of soluble P-Selectin are present in the biological fluids of apparently normal individuals. Furthermore, a number of studies have reported that levels of P-Selectin may be elevated or lowered in subjects with a variety of pathological conditions.

Publications for P-Selectin/CD62P (ADP3)(18)

Publications using ADP3

• Koshrovski-Michael, S;Ajamil, DR;Dey, P;Kleiner, R;Tevet, S;Epshtein, Y;Green Buzhor, M;Khoury, R;Pozzi, S;Shenbach-Koltin, G;Yeini, E;Woythe, L;Blau, R;Scomparin, A;Barshack, I;Florindo, HF;Lazar, S;Albertazzi, L;Amir, RJ;Satchi-Fainaro, R; Two-in-one nanoparticle platform induces a strong therapeutic effect of targeted therapies in P-selectin-expressing cancers Science advances 2024-12-13 [PMID: 39671487] (Bioassay, N/A)
• Datta, R;Miskolci, V;Gallego-López, GM;Britt, E;Gillette, A;Kralovec, A;Giese, MA;Qian, T;Fan, J;Huttenlocher, A;Skala, MC; Single cell autofluorescence imaging reveals immediate metabolic shifts of neutrophils with activation across biological systems bioRxiv : the preprint server for biology 2024-07-29 [PMID: 39211087] (Immunocytochemistry, Human)
• E Yeini, P Ofek, S Pozzi, N Albeck, D Ben-Shusha, G Tiram, S Golan, R Kleiner, R Sheinin, S Israeli Da, S Reich-Zeli, R Grossman, Z Ram, H Brem, TM Hyde, P Magod, D Friedmann-, A Madi, R Satchi-Fai P-selectin axis plays a key role in microglia immunophenotype and glioblastoma progression Nature Communications, 2021-03-26;12(1):1912. 2021-03-26 [PMID: 33771989] (Bioassay, Human)
• R Fitterer, T Lajqi, SA Kranig, M Braun, N Theissig, N Kuss, J Pöschl, D Frommhold, H Hudalla L-Arginine Modulates Neonatal Leukocyte Recruitment in a Gestational Age-Dependent Manner J Clin Med, 2020-08-27;9(9):. 2020-08-27 [PMID: 32867030] (Bioassay, Human)
• V Rolfes, LS Ribeiro, I Hawwari, L Böttcher, N Rosero, S Maasewerd, MLS Santos, T Próchnicki, CMS Silva, CWS Wanderley, M Rothe, SV Schmidt, HJ Stunden, D Bertheloot, MN Rivas, CJ Fontes, LH Carvalho, FQ Cunha, E Latz, M Arditi, BS Franklin Platelets Fuel the Inflammasome Activation of Innate Immune Cells Cell Rep, 2020-05-12;31(6):107615. 2020-05-12 [PMID: 32402278] (Cell Culture, Human)
• S Schwarz, LM Gockel, A Naggi, U Barash, M Gobec, G Bendas, M Schlesinge Glycosaminoglycans as Tools to Decipher the Platelet Tumor Cell Interaction: A Focus on P-Selectin Molecules, 2020-02-26;25(5):. 2020-02-26 [PMID: 32110917] (Bioassay, Human)
• Z Ye, L Zhong, S Zhu, Y Wang, J Zheng, S Wang, J Zhang, R Huang The P-selectin and PSGL-1 axis accelerates atherosclerosis via activation of dendritic cells by the TLR4 signaling pathway Cell Death Dis, 2019-07-01;10(7):507. 2019-07-01 [PMID: 31263109] (Bioassay, Human)
• J Perdomo, HHL Leung, Z Ahmadi, F Yan, JJH Chong, FH Passam, BH Chong Neutrophil activation and NETosis are the major drivers of thrombosis in heparin-induced thrombocytopenia Nat Commun, 2019-03-21;10(1):1322. 2019-03-21 [PMID: 30899022] (Bioassay, Human)
• MJ Domínguez-, E Armas-Gonz, A Herrera-Ga, M Arce-Franc, M Feria, M Vicente-Ma, A Martínez-R, F Sánchez-Ma, F Díaz-Gonzá L-selectin expression is regulated by CXCL8-induced reactive oxygen species produced during human neutrophil rolling Eur. J. Immunol., 2018-12-05;0(0):. 2018-12-05 [PMID: 30443903] (Bioassay, Human)
• Soluble Siglec-5 associates to PSGL-1 and displays anti-inflammatory activity Sci Rep, 2016-11-28;6(0):37953. 2016-11-28 [PMID: 27892504] (ELISA Developmet)
• Platelets Guide Leukocytes to Their Sites of Extravasation PLoS Biol, 2016-05-06;14(5):e1002459. 2016-05-06 [PMID: 27152726] (Bioassay, Human)
• Jung C, Yeh C, Hsu R, Lee C, Shun C, Chia J Endocarditis pathogen promotes vegetation formation by inducing intravascular neutrophil extracellular traps through activated platelets. Circulation, 2014-12-19;131(6):571-81. 2014-12-19 [PMID: 25527699] (Bioassay, Human)
• Jang M, Kim Y, Bae E, Oh T, Choi H, Lee J, Oh H, Lee S Saikosaponin D isolated from Bupleurum falcatum inhibits selectin-mediated cell adhesion. Molecules, 2014-12-04;19(12):20340-9. 2014-12-04 [PMID: 25486247] (Bioassay, Human)
• Schneider-Hohendorf T, Rossaint J, Mohan H, Boning D, Breuer J, Kuhlmann T, Gross C, Flanagan K, Sorokin L, Vestweber D, Zarbock A, Schwab N, Wiendl H VLA-4 blockade promotes differential routes into human CNS involving PSGL-1 rolling of T cells and MCAM-adhesion of TH17 cells. J Exp Med, 2014-08-18;211(9):1833-46. 2014-08-18 [PMID: 25135296] (Bioassay, Human)
• Frommhold D, Kamphues A, Hepper I, Pruenster M, Lukic IK, Socher I, Zablotskaya V, Buschmann K, Lange-Sperandio B, Schymeinsky J, Ryschich E, Poeschl J, Kupatt C, Nawroth PP, Moser M, Walzog B, Bierhaus A, Sperandio M RAGE and ICAM-1 cooperate in mediating leukocyte recruitment during acute inflammation in vivo. Blood, 2010-04-20;116(5):841-9. 2010-04-20 [PMID: 20407037] (Bioassay, Human)
• Deban L, Russo RC, Sironi M, Moalli F, Scanziani M, Zambelli V, Cuccovillo I, Bastone A, Gobbi M, Valentino S, Doni A, Garlanda C, Danese S, Salvatori G, Sassano M, Evangelista V, Rossi B, Zenaro E, Constantin G, Laudanna C, Bottazzi B, Mantovani A Regulation of leukocyte recruitment by the long pentraxin PTX3. Nat. Immunol., 2010-03-07;11(4):328-34. 2010-03-07 [PMID: 20208538] (Binding Assay, Human)
• Wojciechowski JC, Narasipura SD, Charles N, Mickelsen D, Rana K, Blair ML, King MR Capture and enrichment of CD34-positive haematopoietic stem and progenitor cells from blood circulation using P-selectin in an implantable device. Br. J. Haematol., 2008-01-22;140(6):673-81. 2008-01-22 [PMID: 18218048] (In Vivo, Rat)
• Bestebroer J, Poppelier MJ, Ulfman LH, Lenting PJ, Denis CV, van Kessel KP, van Strijp JA, de Haas CJ Staphylococcal superantigen-like 5 binds PSGL-1 and inhibits P-selectin-mediated neutrophil rolling. Blood, 2007-04-01;109(7):2936-43. 2007-04-01 [PMID: 17132726] (Bioassay, Human)

Bioinformatics

• Gene Symbol: SELP
• Uniprot:
  • P16109()