Recombinant Human P-Selectin/CD62P Protein, CF

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Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

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Recombinant Human P-Selectin/CD62P Protein, CF Summary

Details of Functionality
Measured by the ability of the immobilized protein to support the adhesion of U937 human histiocytic lymphoma cells. When 5 x 104 cells/well are added to human sP-Selectin coated plates (10 μg/mL with 100 μL/well), >50% will adhere after 1 hour incubation at room temperature.
Source
Chinese Hamster Ovary cell line, CHO-derived human P-Selectin/CD62P protein
Trp42-Ala771
Accession #
N-terminal Sequence
Trp42
Protein/Peptide Type
Recombinant Proteins
Gene
SELP
Purity
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
80 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
121-124 kDa, reducing conditions
Publications
Read Publications using
ADP3 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS containing Calcium and Magnesium with Sorbitol.
Purity
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 1 mg/mL in sterile, deionized water.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human P-Selectin/CD62P Protein, CF

  • CD62P antigen
  • CD62P
  • FLJ45155
  • GMP140
  • GRMP
  • PADGEM
  • PADGEMantigen CD62)
  • PSEL
  • P-Selectin
  • selectin P (granule membrane protein 140kDa, antigen CD62)
  • SELP

Background

Human P-Selectin (GMP-140, LECAM-3, PADGEM, CD62P), a member of the Selectin family, is a cell surface glycoprotein expressed by activated platelets and endothelial cells. P-Selectin is translocated to the cell surface within minutes, from alpha granules of platelets or Weibel-Palade bodies of endothelial cells, following stimulation with thrombin, histamine, PMA or peroxides. P-Selectin binds to a 106 kDa protein present on myeloid cells, neutrophils, monocytes and lymphocytes, termed PSGL-1 (P-Selectin glycoprotein ligand-1).

P-Selectin plays a role in the adhesion of leukocytes and neutrophils to the endothelium. Acting in cooperation with L Selectin,P-Selectin mediates the initial interaction of circulating leukocytes with endothelial cells that produces a characteristic ‘rolling' of the leukocytes on the endothelium. This initial interaction is followed by a stronger interaction involving E-Selectin, and later ICAM-1 and VCAM-1, that leads eventually to extravasation of the white blood cell through the blood vessel wall into the extracellular matrix tissue.

ELISA techniques have shown that detectable levels of soluble P-Selectin are present in the biological fluids of apparently normal individuals. Furthermore, a number of studies have reported that levels of P-Selectin may be elevated or lowered in subjects with a variety of pathological conditions.

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Publications for P-Selectin/CD62P (ADP3)(9)

We have publications tested in 3 confirmed species: Human, Rat, N/A.

We have publications tested in 4 applications: Binding Assay, Bioassay, ELISA development, In Vivo.


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Binding Assay
(1)
Bioassay
(6)
ELISA development
(1)
In Vivo
(1)
All Applications
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Human
(7)
Rat
(1)
N/A
(1)
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Showing Publications 1 - 9 of 9.
Publications using ADP3 Applications Species
Soluble Siglec-5 associates to PSGL-1 and displays anti-inflammatory activity Sci Rep, 2016;6(0):37953. 2016 [PMID: 27892504] (ELISA development, N/A) ELISA development N/A
Platelets Guide Leukocytes to Their Sites of Extravasation PLoS Biol, 2016;14(5):e1002459. 2016 [PMID: 27152726] (Bioassay, Human) Bioassay Human
Jung C, Yeh C, Hsu R, Lee C, Shun C, Chia J Endocarditis pathogen promotes vegetation formation by inducing intravascular neutrophil extracellular traps through activated platelets. Circulation, 2015;131(6):571-81. 2015 [PMID: 25527699] (Bioassay, Human) Bioassay Human
Jang M, Kim Y, Bae E, Oh T, Choi H, Lee J, Oh H, Lee S Saikosaponin D isolated from Bupleurum falcatum inhibits selectin-mediated cell adhesion. Molecules, 2014;19(12):20340-9. 2014 [PMID: 25486247] (Bioassay, Human) Bioassay Human
Schneider-Hohendorf T, Rossaint J, Mohan H, Boning D, Breuer J, Kuhlmann T, Gross C, Flanagan K, Sorokin L, Vestweber D, Zarbock A, Schwab N, Wiendl H VLA-4 blockade promotes differential routes into human CNS involving PSGL-1 rolling of T cells and MCAM-adhesion of TH17 cells. J Exp Med, 2014;211(9):1833-46. 2014 [PMID: 25135296] (Bioassay, Human) Bioassay Human
Frommhold D, Kamphues A, Hepper I, Pruenster M, Lukic IK, Socher I, Zablotskaya V, Buschmann K, Lange-Sperandio B, Schymeinsky J, Ryschich E, Poeschl J, Kupatt C, Nawroth PP, Moser M, Walzog B, Bierhaus A, Sperandio M RAGE and ICAM-1 cooperate in mediating leukocyte recruitment during acute inflammation in vivo. Blood, 2010;116(5):841-9. 2010 [PMID: 20407037] (Bioassay, Human) Bioassay Human
Deban L, Russo RC, Sironi M, Moalli F, Scanziani M, Zambelli V, Cuccovillo I, Bastone A, Gobbi M, Valentino S, Doni A, Garlanda C, Danese S, Salvatori G, Sassano M, Evangelista V, Rossi B, Zenaro E, Constantin G, Laudanna C, Bottazzi B, Mantovani A Regulation of leukocyte recruitment by the long pentraxin PTX3. Nat. Immunol., 2010;11(4):328-34. 2010 [PMID: 20208538] (Binding Assay, Human) Binding Assay Human
Wojciechowski JC, Narasipura SD, Charles N, Mickelsen D, Rana K, Blair ML, King MR Capture and enrichment of CD34-positive haematopoietic stem and progenitor cells from blood circulation using P-selectin in an implantable device. Br. J. Haematol., 2008;140(6):673-81. 2008 [PMID: 18218048] (In Vivo, Rat) In Vivo Rat
Bestebroer J, Poppelier MJ, Ulfman LH, Lenting PJ, Denis CV, van Kessel KP, van Strijp JA, de Haas CJ Staphylococcal superantigen-like 5 binds PSGL-1 and inhibits P-selectin-mediated neutrophil rolling. Blood, 2007;109(7):2936-43. 2007 [PMID: 17132726] (Bioassay, Human) Bioassay Human

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Bioinformatics

Gene Symbol SELP
Uniprot