Recombinant Human P-Selectin/CD62P His-tag Protein, CF

Immobilized Recombinant Human P-Selectin/CD62P His-tag Protein (Catalog # 11035-PS) supports the adhesion of U937 human histiocytic lymphoma cells. The ED50 for this effect is 0.200-1.00 ug/mL.

2 μg/lane of Recombinant Human P-Selectin/CD62P His-tag Protein (Catalog # 11035-PS) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 105-125- kDa.

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human P-Selectin/CD62P His-tag Protein, CF Summary

• Details of Functionality: Measured by the ability of the immobilized protein to support the adhesion of U937 human histiocytic lymphoma cells. The ED₅₀ for this effect is 0.200-1.00 μg/mL.
• Source: Human embryonic kidney cell, HEK293-derived human P-Selectin/CD62P protein
  Trp42-Ala771, with a C-terminal 6-His tag
• Accession #: NP_002996.2
• N-terminal Sequence: Trp42
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 81 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 105-125 kDa, under reducing conditions.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human P-Selectin/CD62P His-tag Protein, CF

• CD62P antigen
• CD62P
• FLJ45155
• GMP140
• GRMP
• PADGEM
• PADGEMantigen CD62)
• PSEL
• P-Selectin
• selectin P (granule membrane protein 140kDa, antigen CD62)
• SELP

Background

P-Selectin, also known as GMP-140, PADGEM, and CD62P, is a cell surface glycoprotein of the Selectin family and is expressed by activated platelets and endothelial cells (1 - 3). In humans, there are 3 Selectins, P, E, and L and they are Ca2+-dependent lectins that help mediate the initial adhesive step during inflammation and immune surveillance (4). Mature human P-Selectin consists of an extracellular domain (ECD) with a C‑type lectin domain and an EGF-like domain followed by a series of complement factor A repeat homology domains, a transmembrane domain and a short cytoplasmic domain (5). The mature ECD of human P-selectin shares 66% amino acid sequence identity with mouse P-Selectin. P-Selectin is translocated to the cell surface within minutes, from alpha granules of platelets or Weibel-Palade bodies of endothelial cells, following stimulation with thrombin, histamine, PMA or peroxides (6). P-Selectin binds to P-Selectin glycoprotein ligand-1 (PSGL-1), a dimeric molecule rich in O- and N-glycans, present on myeloid cells, neutrophils, monocytes and lymphocytes (7). P-Selectin plays a role in the adhesion of leukocytes and neutrophils to the endothelium. Acting in cooperation with L-Selectin, P-Selectin mediates the initial interaction of circulating leukocytes with endothelial cells that produces a characteristic ‘rolling' of the leukocytes on the endothelium (8). This initial interaction is followed by a stronger interaction involving E‑Selectin, and later ICAM-1 and VCAM-1, that leads eventually to extravasation of the white blood cell through the blood vessel wall into the extracellular matrix tissue (1, 3).

1. Kansas, G.S. (1996) Blood 88:3259.
2. Lü, S. (2015) PLOS One 10:1.
3. Edwards, E.E. (2017) Integr. Biol. (Camb) 9:313.
4. McEver, R.P. (1997) J. Clin. Invest. 100:485.
5. Patel, K.D. (1995) J. Cell Biol. 131:1893.
6. Zhang, N. (2016) Arterioscler Thromb. Vasc. Biol 36:1114.
7. Frenette, P.S. et al. (2000) J Exp. Med. 191:1413.
8. Zuchtriegel, G. (2016) PLOS Biology 14:1.

Publications for P-Selectin/CD62P (11035-PS)(1)

Publication using 11035-PS

• Ruzemaimaiti, D;Sun, H;Zhang, J;Xu, C;Chen, L;Yin, R;Zhao, J; Oligomer-guided recognition of two fucan sulfate from Bohadschia argus and inhibition of P-selectin binding to its ligand Carbohydrate polymers 2023-10-01 [PMID: 37364953] (ELISA Capture, N/A)

Bioinformatics

• Uniprot:
  • NP_002996.2()