Recombinant Human NT-3 Protein, CF

Recombinant Human NT‑3 Protein (Catalog # 11563-N3) stimulates cell proliferation in the BaF mouse pro-B cell line transfected with TrkB. The ED50 for this effect is 5.00-70.0 ng/mL.

2 μg/lane of Recombinant Human NT‑3 Protein (Catalog # 11563-N3) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 12-15 kDa, under reducing conditions.

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human NT-3 Protein, CF Summary

• Additional Information: (CHO expressed)
• Details of Functionality: Measured in a cell proliferation assay using BaF mouse pro-B cells transfected with TrkB. The ED₅₀ for this effect is 5.00-70.0 ng/mL.
• Source: Chinese Hamster Ovary cell line, CHO-derived human NT-3 protein
  Tyr139-Thr257
• Accession #: P20783.1
• N-terminal Sequence: Tyr139
• Protein/Peptide Type: Recombinant Proteins
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 14 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 12-15 kDa, under reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Reconstitution Instructions: Reconstitute the 10 μg size at 100 μg/mL in PBS. Reconstitute all other sizes at 250 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human NT-3 Protein, CF

• HDNF
• MGC129711
• Nerve growth factor 2
• Neurotrophic factor
• neurotrophin 3
• neurotrophin-3
• NGF2
• NGF-2
• NT3
• NT-3
• NTF3

Background

Neurotrophin-3 (NT-3) is a member of the NGF family of neurotrophic factors (also named neurotrophins) that are required for the differentiation and survival of specific neuronal subpopulations in both the central as well as the peripheral nervous systems. The neurotrophin family is comprised of at least four proteins including NGF, BDNF, NT-3, and NT-4/5. These secreted cytokines are synthesized as prepropeptides that are proteolytically processed to generate the mature proteins. All neurotrophins have six conserved cysteine residues that are involved in the formation of three disulfide bonds and all share approximately 55% sequence identity at the amino acid level. Similarly to NGF, bioactive NT-3 is predicted to be a non-covalently linked homodimer.

The NT-3 cDNA encodes a 257 amino acid residue precursor protein with a signal peptide and a proprotein that are cleaved to yield the 119 amino acid residue mature NT-3. The amino acid sequence of mature NT-3 is identical in human, mouse and rat. NT-3 transcripts have been detected in the cerebellum, hippocampus, placenta, heart, skin, and skeletal muscle. NT-3 primarily activates the TrkC tyrosine kinase receptor. In addition, NT-3 can activate Trk and TrkB kinase receptors in certain cell systems. NT-3 can also bind with low affinity to the low affinity NGF receptor.

1. Eide, F.F. et al. (1993) Exp. Neurol. 121:200.
2. Snider, W.D. (1994) Cell 77:627.
3. Barbacid, M. (1994) J. Neurobiol. 25:1386.

Bioinformatics

• Uniprot:
  • P20783.1()