Recombinant Human NKp80/KLRF1 Fc Chimera Protein, CF

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human NKp80/KLRF1 Fc Chimera Protein, CF Summary

• Details of Functionality: Measured by the ability of the immobilized protein to support the adhesion of U937 human histiocytic lymphoma cells. Welte, S. et al. (2006) Nat. Immunol. 7:12. rhNKp80/Fc Chimera, immobilized at 5 µg/mL (100 µL/well), can induce greater than 40% of U937 cell adhesion.
• Source: Mouse myeloma cell line, NS0-derived human NKp80/KLRF1 protein
  

  MD	Human IgG1 (Pro100 - Lys330)	IEGR	Human NKp80 (Val66 - Tyr231) Accession # Q9NZS2
  N-terminus			C-terminus

• Accession #: Q9NZS2
• N-terminal Sequence: Met
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Gene: KLRF1
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 45.8 kDa (monomer).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 59-62 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human NKp80/KLRF1 Fc Chimera Protein, CF

• Activating coreceptor NKp80
• CLEC5C
• CLEC5CMGC119908
• C-type lectin domain family 5 member C
• killer cell lectin-like receptor F1
• killer cell lectin-like receptor subfamily F member 1
• killer cell lectin-like receptor subfamily F, member 1
• KLRF1
• Lectin-like receptor F1
• MGC119907
• MGC119909
• ML
• NKp80

Background

NKp80, also known as killer cell lectin-like receptor subfamily F member 1 (KLRF1), is an 80 kDa type II transmembrane, homodimeric, activating coreceptor expressed exclusively on the surface of NK cells. Human NKp80, a member of the C-type lectin family of proteins (1 - 3), is 231 aa in length with a 38 amino acid (aa) cytoplasmic region, a 21 aa transmembrane region, and a 172 aa extracellular domain (ECD) (SwissProt # Q9NZS2). The ECD contains the C-type lectin-like domain (aa 114 - 230) and four potential sites of N-linked glycosylation. Multiple splicing variants produce four isoforms for NKp80. Isoform 1 is the standard protein. There are no murine orthologs for NKp80 (4). One early study suggested that NKp80 functions as an inhibitory receptor based on the presence of two immunoreceptor tyrosine-based inhibitory motifs (ITIM) in the N-terminal cytoplasmic region, and the lack of charged amino acids in the transmembrane region (1). However, later studies show that NKp80 displays an activating function rather than an inhibitory one (2 - 3, 5). In in vitro experiments in human NK cells, NKp80 stimulates NK cell cytotoxicity and induces calcium influx after triggering by appropriate antibodies (2 - 3). One study reports that NKp80 likely functions as a coreceptor, cooperating with NKp46 and other activating receptors in NK cell cytotoxicity (2). Activation-induced C-type lectin (AICL) is the ligand for NKp80 (3). It is a myeloid-specific receptor expressed by monocytes, macrophages, and granulocytes (3). Crosslinking of both NKp80 and AICL stimulates secretion of proinflammatory cytokines suggesting that NKp80-AICL interaction is involved in the activating crosstalk between NK cells and myeloid cells, and thus may influence the initiation and maintenance of immune responses in humans (3).

1. Roda-Navarro, P. et al. (2000) Eur. J. Immunol. 30:568.
2. Vitale, M. et al. (2001) Eur. J. Immunol. 31:233.
3. Welte, S. et al. (2006) Nat. Immunol. 7:1334.
4. Yokoyama, W.M. and B.F. Plougastel (2003) Nat. Rev. Immunol. 3:304.
5. Thomas, M. et al. (2008) Proc. Natl. Acad. Sci. U.S.A. 105:1656.

Bioinformatics

• Gene Symbol: KLRF1
• Uniprot:
  • Q9NZS2(Human)