Recombinant Human NKG2D Fc Chimera Protein, CF

Images

 
There are currently no images for NKG2D/CD314 (1299-NK).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Binding Activity
Format
Carrier-Free

Order Details

Recombinant Human NKG2D Fc Chimera Protein, CF Summary

Details of Functionality
Measured by its ability to bind its ligand, rhMICA/Fc Chimera in a functional ELISA.
Source
Mouse myeloma cell line, NS0-derived human NKG2D/CD314 protein
MD Human IgG1
(Pro100-Lys330)
IEGR Human NKG2D
(Phe78-Val216)
Accession # P26718
N-terminus C-terminus
Accession #
N-terminal Sequence
Met
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
KLRK1
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
43 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
50-55 kDa, reducing conditions
Publications
Read Publications using
1299-NK in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human NKG2D Fc Chimera Protein, CF

  • CD314 antigen
  • CD314
  • D12S2489E
  • FLJ17759
  • FLJ75772
  • Killer cell lectin-like receptor subfamily K member 1
  • killer cell lectin-like receptor subfamily K, member 1
  • KLR
  • KLRK1
  • NK cell receptor D
  • NKG2-D type II integral membrane protein
  • NKG2D
  • NKG2-D
  • NKG2-D-activating NK receptor
  • NKG2DDNA segment on chromosome 12 (unique) 2489 expressed sequence

Background

NKG2D is a type II transmembrane glycoprotein having an extracellular lectin-like domain. This domain lacks the recognizable calcium-binding sites found in true C-type lectins and binds protein rather than carbohydrate ligands. Human NKG2D is expressed on CD8+ alpha beta T cells, gamma δ T cells, NK cells and NKT cells. In mouse systems NKG2D also occurs on macrophages. Human ligands for NKG2D include MICA, MICB, and ULBP1, 2, and 3. Expression of NKG2D ligands occurs in epithelial cells, tumor cells and under conditions of stress or infection. NKG2D exists as a disulfide-linked homodimer that delivers an activating signal upon ligand binding. Signaling requires association with an adapter protein. Alternative splicing of the NKG2D mRNA results in isoforms with different cytoplasmic domains that can associate either with DAP12 to deliver a true activating signal or with DAP10 resulting in a costimulatory signal. NKG2D has been implicated in anti-tumor surveillance and the immune response against viral infection.

  1. Li, P. et al. (2001) Nature Immunol. 2:443.
  2. Steinle, A. et al. (2001) Immunogenetics 53:279.
  3. Cosman, D. et al. (2001) Immunity 14:123.
  4. Cerwenka, A. and L. Lanier (2001) Immunol. Rev. 181:158.
  5. Wu, J. et al. (1999) Science 285:730.
  6. Diefenbach, A. et al. (2002) Nature Immunol. 3:1142.
  7. Gilfillan, S. et al. (2002) Nature Immunol. 3:1150.
  8. Groh, V. et al. (2001) Nature Immunol. 2:255.
  9. Cerwenka, A. et al. (2001) Proc. Natl. Acad. Sci. USA 98:11521.
  10. Diefenbach, A. et al. (2001) Nature 413:165.
  11. NKG2D and its Ligands (2002) www.rndsystems.com

Customers Who Viewed This Item Also Viewed...

AF2225
Species: Mu
Applications: WB, Flow, IHC, AgAct, CyTOF-ready
MAB1058
Species: Hu
Applications: Flow, Block, CyTOF-ready
NBP1-49045
Species: Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, Cell Depl, CyTOF-ready, InhibTFunc
1849-NK
Species: Hu
2249-NK
Species: Hu
MAB1599
Species: Hu
Applications: WB, Flow, CyTOF-ready, ELISA(Cap), ELISA(Det), ELISA(Sta)
AF-386-PB
Species: Hu
Applications: WB, Flow, CyTOF-ready, ICC
MAB97861
Species: Hu
Applications: Flow, IHC
NB100-524
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, Flow-IC
NBP1-84550
Species: Hu
Applications: WB, IHC, IHC-P
NBP2-15184
Species: Hu, Rt, Ze
Applications: WB, Simple Western, ELISA, Flow, ICC/IF, IHC-Fr, IHC-P, IP, PAGE
MAB1380
Species: Hu
Applications: Flow, Block, CyTOF-ready
NB120-6405
Species: Rt
Applications: B/N, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, CyTOF-ready, Flow-IC
MAB666
Species: Hu
Applications: WB, Flow, CyTOF-ready, Neut

Publications for NKG2D/CD314 (1299-NK)(22)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 5 applications: Bioassay, Blocking, Flow, Flow Ctrl, Isothermal Microcalorimetry.


Filter By Application
Bioassay
(6)
Blocking
(1)
Flow
(13)
Flow Ctrl
(1)
Isothermal Microcalorimetry
(1)
All Applications
Filter By Species
Human
(21)
Mouse
(1)
All Species
Showing Publications 1 - 10 of 22. Show All 22 Publications.
Publications using 1299-NK Applications Species
J Liu, L Wang, Y Wang, W Zhang, Y Cao Phenotypic characterization and anticancer capacity of CD8+ cytokine-induced killer cells after antigen-induced expansion PLoS ONE, 2017;12(4):e0175704. 2017 [PMID: 28426690] (Bioassay, Human) Bioassay Human
Eggink L, Spyroulias G, Jones N, Hanson C, Hoober J A Peptide Mimetic of 5-Acetylneuraminic Acid-Galactose Binds with High Avidity to Siglecs and NKG2D. PLoS ONE, 2015;10(6):e0130532. 2015 [PMID: 26110603] (Isothermal Microcalorimetry, Human) Isothermal Microcalorimetry Human
Kruse V, Hamann C, Monecke S, Cyganek L, Elsner L, Hubscher D, Walter L, Streckfuss-Bomeke K, Guan K, Dressel R Human Induced Pluripotent Stem Cells Are Targets for Allogeneic and Autologous Natural Killer (NK) Cells and Killing Is Partly Mediated by the Activating NK Receptor DNAM-1. PLoS ONE, 2015;10(5):e0125544. 2015 [PMID: 25950680] (Flow, Human) Flow Human
Cho, Hanbyoul, Chung, Joon-Yon, Kim, Sunghoon, Braunschweig, Till, Kang, Tae Heun, Kim, Jennie, Chung, Eun Joo, Hewitt, Stephen, Kim, Jae-Hoon MICA/B and ULBP1 NKG2D ligands are independent predictors of good prognosis in cervical cancer. BMC Cancer, 2014;14(0):957. 2014 [PMID: 25510288] (Flow, Human) Flow Human
Mou, Xiao, Zhou, Yuepeng, Jiang, Peng, Zhou, Tong, Jiang, Qian, Xu, Chengche, Liu, Hongli, Zheng, Tingting, Yuan, Guoyue, Zhang, Yanyun, Chen, Deyu, Mao, Chaoming The regulatory effect of UL-16 binding protein-3 expression on the cytotoxicity of NK cells in cancer patients. Sci Rep, 2014;4(0):6138. 2014 [PMID: 25138242] (Bioassay, Human) Bioassay Human
Uhlenbrock F, Hagemann-Jensen M, Kehlet S, Andresen L, Pastorekova S, Skov S The NKG2D ligand ULBP2 is specifically regulated through an invariant chain-dependent endosomal pathway. J Immunol, 2014;193(4):1654-65. 2014 [PMID: 25024379] (Blocking, Human) Blocking Human
Mellergaard M, Skovbakke S, Schneider C, Lauridsen F, Andresen L, Jensen H, Skov S N-glycosylation of asparagine 8 regulates surface expression of major histocompatibility complex class I chain-related protein A (MICA) alleles dependent on threonine 24. J Biol Chem, 2014;289(29):20078-91. 2014 [PMID: 24872415] (Flow, Human) Flow Human
Schlecker E, Fiegler N, Arnold A, Altevogt P, Rose-John S, Moldenhauer G, Sucker A, Paschen A, von Strandmann E, Textor S, Cerwenka A Metalloprotease-mediated tumor cell shedding of B7-H6, the ligand of the natural killer cell-activating receptor NKp30. Cancer Res, 2014;74(13):3429-40. 2014 [PMID: 24780758] (Flow Ctrl, Human) Flow Ctrl Human
Nakata J, Nakano K, Okumura A, Mizutani Y, Kinoshita H, Iwai M, Hasegawa K, Morimoto S, Fujiki F, Tatsumi N, Nakajima H, Nakae Y, Nishida S, Tsuboi A, Oji Y, Oka Y, Sugiyama H, Kumanogoh A, Hosen N In vivo eradication of MLL/ENL leukemia cells by NK cells in the absence of adaptive immunity. Leukemia, 2014;28(6):1316-25. 2014 [PMID: 24336127] (Bioassay, Mouse) Bioassay Mouse
Rothe A, Jachimowicz R, Borchmann S, Madlener M, Kessler J, Reiners K, Sauer M, Hansen H, Ullrich R, Chatterjee S, Borchmann P, Yazaki P, Koslowsky T, Engert A, Heukamp L, Hallek M, von Strandmann E The bispecific immunoligand ULBP2-aCEA redirects natural killer cells to tumor cells and reveals potent anti-tumor activity against colon carcinoma. Int J Cancer, 2014;134(12):2829-40. 2014 [PMID: 24242212] (Bioassay, Human) Bioassay Human
Show All 22 Publications.

Reviews for NKG2D/CD314 (1299-NK) (0)

There are no reviews for NKG2D/CD314 (1299-NK). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

FAQs for NKG2D/CD314 (1299-NK) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Recombinant Human NKG2D Fc Chimera Protein, CF and receive a gift card or discount.

Bioinformatics

Gene Symbol KLRK1
Entrez
Uniprot