Recombinant Human NKG2D Fc Chimera Protein, CF


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Product Details

Reactivity HuSpecies Glossary
Applications Binding Activity

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Recombinant Human NKG2D Fc Chimera Protein, CF Summary

Details of Functionality
Measured by its ability to bind its ligand, rhMICA/Fc Chimera in a functional ELISA.
Mouse myeloma cell line, NS0-derived human NKG2D/CD314 protein
MD Human IgG1
Accession # P26718
N-terminus C-terminus
Accession #
N-terminal Sequence
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.


Theoretical MW
43 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
50-55 kDa, reducing conditions
Read Publications using
1299-NK in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS.
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human NKG2D Fc Chimera Protein, CF

  • CD314 antigen
  • CD314
  • D12S2489E
  • FLJ17759
  • FLJ75772
  • Killer cell lectin-like receptor subfamily K member 1
  • killer cell lectin-like receptor subfamily K, member 1
  • KLR
  • KLRK1
  • NK cell receptor D
  • NKG2-D type II integral membrane protein
  • NKG2D
  • NKG2-D
  • NKG2-D-activating NK receptor
  • NKG2DDNA segment on chromosome 12 (unique) 2489 expressed sequence


NKG2D is a type II transmembrane glycoprotein having an extracellular lectin-like domain. This domain lacks the recognizable calcium-binding sites found in true C-type lectins and binds protein rather than carbohydrate ligands. Human NKG2D is expressed on CD8+ alpha beta T cells, gamma δ T cells, NK cells and NKT cells. In mouse systems NKG2D also occurs on macrophages. Human ligands for NKG2D include MICA, MICB, and ULBP1, 2, and 3. Expression of NKG2D ligands occurs in epithelial cells, tumor cells and under conditions of stress or infection. NKG2D exists as a disulfide-linked homodimer that delivers an activating signal upon ligand binding. Signaling requires association with an adapter protein. Alternative splicing of the NKG2D mRNA results in isoforms with different cytoplasmic domains that can associate either with DAP12 to deliver a true activating signal or with DAP10 resulting in a costimulatory signal. NKG2D has been implicated in anti-tumor surveillance and the immune response against viral infection.

  1. Li, P. et al. (2001) Nature Immunol. 2:443.
  2. Steinle, A. et al. (2001) Immunogenetics 53:279.
  3. Cosman, D. et al. (2001) Immunity 14:123.
  4. Cerwenka, A. and L. Lanier (2001) Immunol. Rev. 181:158.
  5. Wu, J. et al. (1999) Science 285:730.
  6. Diefenbach, A. et al. (2002) Nature Immunol. 3:1142.
  7. Gilfillan, S. et al. (2002) Nature Immunol. 3:1150.
  8. Groh, V. et al. (2001) Nature Immunol. 2:255.
  9. Cerwenka, A. et al. (2001) Proc. Natl. Acad. Sci. USA 98:11521.
  10. Diefenbach, A. et al. (2001) Nature 413:165.
  11. NKG2D and its Ligands (2002)

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Publications for NKG2D/CD314 (1299-NK)(22)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 5 applications: Bioassay, Blocking, Flow, Flow Ctrl, Isothermal Microcalorimetry.

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Flow Ctrl
Isothermal Microcalorimetry
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Showing Publications 1 - 10 of 22. Show All 22 Publications.
Publications using 1299-NK Applications Species
J Liu, L Wang, Y Wang, W Zhang, Y Cao Phenotypic characterization and anticancer capacity of CD8+ cytokine-induced killer cells after antigen-induced expansion PLoS ONE, 2017;12(4):e0175704. 2017 [PMID: 28426690] (Bioassay, Human) Bioassay Human
Eggink L, Spyroulias G, Jones N, Hanson C, Hoober J A Peptide Mimetic of 5-Acetylneuraminic Acid-Galactose Binds with High Avidity to Siglecs and NKG2D. PLoS ONE, 2015;10(6):e0130532. 2015 [PMID: 26110603] (Isothermal Microcalorimetry, Human) Isothermal Microcalorimetry Human
Kruse V, Hamann C, Monecke S, Cyganek L, Elsner L, Hubscher D, Walter L, Streckfuss-Bomeke K, Guan K, Dressel R Human Induced Pluripotent Stem Cells Are Targets for Allogeneic and Autologous Natural Killer (NK) Cells and Killing Is Partly Mediated by the Activating NK Receptor DNAM-1. PLoS ONE, 2015;10(5):e0125544. 2015 [PMID: 25950680] (Flow, Human) Flow Human
Cho, Hanbyoul, Chung, Joon-Yon, Kim, Sunghoon, Braunschweig, Till, Kang, Tae Heun, Kim, Jennie, Chung, Eun Joo, Hewitt, Stephen, Kim, Jae-Hoon MICA/B and ULBP1 NKG2D ligands are independent predictors of good prognosis in cervical cancer. BMC Cancer, 2014;14(0):957. 2014 [PMID: 25510288] (Flow, Human) Flow Human
Mou, Xiao, Zhou, Yuepeng, Jiang, Peng, Zhou, Tong, Jiang, Qian, Xu, Chengche, Liu, Hongli, Zheng, Tingting, Yuan, Guoyue, Zhang, Yanyun, Chen, Deyu, Mao, Chaoming The regulatory effect of UL-16 binding protein-3 expression on the cytotoxicity of NK cells in cancer patients. Sci Rep, 2014;4(0):6138. 2014 [PMID: 25138242] (Bioassay, Human) Bioassay Human
Uhlenbrock F, Hagemann-Jensen M, Kehlet S, Andresen L, Pastorekova S, Skov S The NKG2D ligand ULBP2 is specifically regulated through an invariant chain-dependent endosomal pathway. J Immunol, 2014;193(4):1654-65. 2014 [PMID: 25024379] (Blocking, Human) Blocking Human
Mellergaard M, Skovbakke S, Schneider C, Lauridsen F, Andresen L, Jensen H, Skov S N-glycosylation of asparagine 8 regulates surface expression of major histocompatibility complex class I chain-related protein A (MICA) alleles dependent on threonine 24. J Biol Chem, 2014;289(29):20078-91. 2014 [PMID: 24872415] (Flow, Human) Flow Human
Schlecker E, Fiegler N, Arnold A, Altevogt P, Rose-John S, Moldenhauer G, Sucker A, Paschen A, von Strandmann E, Textor S, Cerwenka A Metalloprotease-mediated tumor cell shedding of B7-H6, the ligand of the natural killer cell-activating receptor NKp30. Cancer Res, 2014;74(13):3429-40. 2014 [PMID: 24780758] (Flow Ctrl, Human) Flow Ctrl Human
Nakata J, Nakano K, Okumura A, Mizutani Y, Kinoshita H, Iwai M, Hasegawa K, Morimoto S, Fujiki F, Tatsumi N, Nakajima H, Nakae Y, Nishida S, Tsuboi A, Oji Y, Oka Y, Sugiyama H, Kumanogoh A, Hosen N In vivo eradication of MLL/ENL leukemia cells by NK cells in the absence of adaptive immunity. Leukemia, 2014;28(6):1316-25. 2014 [PMID: 24336127] (Bioassay, Mouse) Bioassay Mouse
Rothe A, Jachimowicz R, Borchmann S, Madlener M, Kessler J, Reiners K, Sauer M, Hansen H, Ullrich R, Chatterjee S, Borchmann P, Yazaki P, Koslowsky T, Engert A, Heukamp L, Hallek M, von Strandmann E The bispecific immunoligand ULBP2-aCEA redirects natural killer cells to tumor cells and reveals potent anti-tumor activity against colon carcinoma. Int J Cancer, 2014;134(12):2829-40. 2014 [PMID: 24242212] (Bioassay, Human) Bioassay Human
Show All 22 Publications.

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Gene Symbol KLRK1