Recombinant Human Netrin-G1a His-tag Protein, CF

When Recombinant Human NGL-1/LRRC4C (Catalog # 4899-NR) is coated at 2 μg/mL, 100 μL/well, Recombinant Human Netrin-G1a (Catalog # 10019-NG) binds with an ED50 of 0.2‑1 μg/mL.

2 μg/lane of Recombinant Human Netrin‑G1a was resolved with SDS-PAGE underreducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Bluestaining, showing bands at 69-76 kDa and 55- 66 kDa, respectively.

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human Netrin-G1a His-tag Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. When Recombinant Human NGL-1/LRRC4C (Catalog # 4899-NR) is coated at 2 μg/mL (100 μL/well), the concentration of Recombinant Human Netrin-G1a that produces 50% optimal binding response is 0.2-1 μg/mL.
• Source: Human embryonic kidney cell, HEK293-derived human Netrin-G1a protein
  His29-Gly503, with a C-terminal 6-His tag
• Accession #: Q9Y2I2
• N-terminal Sequence: Val19 & His29
• Structure / Form: Monomer
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 55 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 69-76 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Netrin-G1a His-tag Protein, CF

• Lmnt1
• NetrinG1a
• Netrin-G1a
• NTG1
• NTNG1

Background

NTNG-1, known as Netrin-G1 or alternatively Laminet-1 (Gene name: NTNG1), is a member of the UNC-6/Netrin family of proteins. Human Netrin-G1 is a 55 kDa protein and shares 100% homology with its crab-eating macaque, 98% homology with its rat homolog, and 97% homology with its mouse homolog. Netrin-G1 is synthesized as a 539 amino acid (aa) precursor with a 28 aa signal sequence, a 251 aa laminin-related region containing an N-terminal laminin globular domain (domain VI) followed by 3 laminin EGF-like repeats, a GPI (glycosylphosphatidylinisotol) anchor (Ser510), and a C-terminal 28 aa propeptide that is removed in mature Netrin-G1 (1, 3, 4). Netrin-G1 interacts with its ligand, Netrin-G ligand 1 (NGL1) to regulate neuron growth and patterning, axonal subdendritic differentiation, and synapse formation throughout development (1, 2, 4, 6). Abnormal expression of Netrin-G1 via SNPs (single nucleotide polymorphisms) have been implicated in pathogenesis for schizophrenia (5). Netrin-G1 has widespread expression only in vertebrates, occurring in olfactory mitral cells, cells of the inferior colliculus (hearing), dorsal thalamus (behavior), and cells of the deep cerebellar nuclei and inferior olive (motion) (3).

1. Seiradake, E. et al. (2011) EMBO J. 30:4479.
2. Song, Y. et al. (2013) J Cell Sci. 126:4926.
3. Nakashiba, T. et al. (2000) J Neuroscience. 20:6540.
4. Matsukawa, H. et al. (2014) J Neuroscience. 34:15779.
5. Zhu, Y. et al. (2011) J Genet. 90:499.
6. Nishimura-Akiyoshi, S. et al. (2007) Neuroscience. 104:14801.

Publications for Netrin-G1a (10019-NG)(1)

Publication using 10019-NG

• N Naldaiz-Ga, M Goicoechea, IM Aragón, V Pérez-Lópe, S Fuertes-Al, B Herrera-Im, A López de M, R de Luna-Di, PM Baptista, MA Fernández, MF Lara, A Izeta Isolation and characterization of myogenic precursor cells from human cremaster muscle Sci Rep, 2019-03-05;9(1):3454. 2019-03-05 [PMID: 30837559] (Bioassay, Human)

Bioinformatics

• Uniprot:
  • Q9Y2I2()