Recombinant Human MSPR/Ron His-tag Avi-tag Protein, CF

When Recombinant Human MSP/MST1 (Cys672Ala) Protein (Catalog # 4306-MS) is immobilized at 0.5 μg/mL, 100 μL/well, the concentration of Biotinylated Recombinant Human MSPR/Ron His-tag Avi-tag that produces 50% of the optimal binding response is approximately 40-240 ng/mL.

2 μg/lane of Recombinant Human MSPR/Ron His-tag Avi-tag Protein (Catalog # AVI1947) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 34-42 and 64-78 kDa.

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human MSPR/Ron His-tag Avi-tag Protein, CF Summary

• Additional Information: Biotinylated
• Details of Functionality: Measured by its binding ability in a functional ELISA. When Recombinant Human MSP/MST1 (Cys672Ala) (Catalog # 4306-MS) is immobilized at 0.5 μg/mL, 100 μL/well, the concentration of Biotinylated Recombinant Human MSPR/Ron His-tag Avi-tag that produces 50% of the optimal binding response is approximately 40‑240 ng/mL.
• Source: Human embryonic kidney cell, HEK293-derived human MSPR/Ron protein
  

  Human MSPR/Ron (Glu25-Leu571) Accession # CAA49634.1	HHHHHH	Avi-tag
  N-terminus		C-terminus

• Accession #: CAA49634.1
• N-terminal Sequence: Glu25 ( alpha chain) and Gly310 ( beta chain)
• Structure / Form: Biotinylated via Avi-tag
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 30 kDa ( alpha chain) & 31 kDa ( beta chain).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 34-42 kDa & 64-78 kDa, under reducing conditions.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 200 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human MSPR/Ron His-tag Avi-tag Protein, CF

• CD136 antigen
• CD136
• CDw136c-met-related tyrosine kinase
• EC 2.7.10
• EC 2.7.10.1
• macrophage stimulating 1 receptor (c-met-related tyrosine kinase)
• MSP R
• MSP receptor
• MSPR
• MST1R
• p185-Ron
• Protein-tyrosine kinase 8
• PTK8 protein tyrosine kinase 8
• PTK8
• Ron
• RONmacrophage-stimulating protein receptor
• soluble RON variant 1
• soluble RON variant 2
• soluble RON variant 3
• soluble RON variant 4

Background

Macrophage stimulating protein receptor (MSPR), encoded by the human Ron and the mouse Stk, is one of a small family of receptor tyrosine kinases (RTKs) that also includes human Met (the receptor for hepatocyte growth factor) and chicken Sea (1, 2). This family of receptors is synthesized as a single-chain precursors that are cleaved into a mature disulfide-linked heterodimers composed of an extracellular alpha chain and a membrane spanning beta chain with intrinsic tyrosine kinase activity. Biologically active ligands (MSP and HGF) for this family of receptors are also disulfide-linked alpha -beta heterodimers. Human MSPR cDNA encodes a 1400 amino acid (aa) residue precursor protein with a 24 aa signal peptide, a 285 aa residue alpha chain (Glu25-Arg309) and a 1091 aa residue transmembrane beta chain (Gly310-Thr1400). The extracellular domain of MSPR is comprised of an N-terminal sema domain, a PSI (plexin semaphorins integrins) domain, followed by four immunoglobulin-like folds shared by plexins and transcription factors (3). The soluble sema domain binds MSP and inhibits the MSPR-dependent signaling pathways. MSP receptor is expressed in multiple tissues including specific areas of the central and peripheral nervous systems, epithelial cells along the digestive tract, skin and lung, and in subpopulations of the mononuclear phagocyte lineage (1, 2). Although free MSP alpha or beta chains have been shown to bind MSPR, only the heterodimeric MSP can induce receptor activation and cause biological activity (4, 5). MSPR associates with other transmembrane molecules including integrins, cadherins and other cytokine receptors. Transactivation and signaling crosstalk between MSPR and its associated transmembrane receptors have been demonstrated (6-8). Human MSPR shares 72% amino acid sequence identity with mouse MSPR.

1. Gaudino, G. et al. (1994) EMBO J. 13:3524.
2. Wang, M-H. et al. (1994) Science 266:117.
3. Angelonis, D. et al. (2004) J. Biol. Chem. 279:3726.
4. Wang, M-H. et al. (1997) J. Biol. Chem. 272:16999.
5. Danilkovitch, A. et al. (1999) J. Biol. Chem. 274:29937.
6. Danilkovitch-Miagkova, A. et al. (2000) J. Biol. Chem. 275:14783.
7. Danilkovitch-Miagkova, A. and A. Leonard (2001) Histol. Histopathol. 16:623.
8. Santora, M. et al. (2003) Devel. Cell 2:257.

Bioinformatics

• Uniprot:
  • CAA49634.1()