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HAF008). A specific band was detected for Phospho-MSP R/Ron (Y1238/Y1239) at approximately 170 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox" target="_blank">
352-MS) for 10 minutes. MSP R/Ron (Y1238/Y1239) was immunoprecipiated with 1 ug Goat Anti-Human MSP R/Ron Antigen Affinity-purified Polyclonal Antibody(Catalog # AF691). The MSP R/Ron (Y1238/Y1239)-antibody complexes were absorbed using Protein G Sepharose. Immunoprecipitated MSP R/Ron (Y1238/Y1239) was detected by Western blot using 0.1 µg/mL of Rabbit Anti-Human Phospho-MSP R/Ron (Y1238/Y1239) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1947)." class="big_lightbox" target="_blank">
352-MS) for 10 minutes. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human MSP R/Ron Antigen Affinity-purified Polyclonal Antibody (Catalog # AF691) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for MSP R/Ron at approximately 150-160 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox" target="_blank">
352-MS) for 10 minutes. MSP R/Ron was immunoprecipiated with 1 ug Goat Anti-Human MSP R/Ron Antigen Affinity-purified Polyclonal Antibody (Catalog # AF691). The MSP R/Ron-antibody complexes were absorbed using Protein G Sepharose. Immunoprecipitated MSP R/Ron was detected by Western blot using 0.2 µg/mL of Goat Anti-Human MSP R/Ron Antigen Affinity-purified Polyclonal Antibody (Catalog # AF691)." class="big_lightbox" target="_blank">
352-MS) for five minutes to induce tyrosine phosphorylation of MSP R. Serial dilutions of lysates were analyzed by (A) IP-Western Blot and (B) this DuoSet® IC ELISA. IPs were performed using an anti-MSP R monoclonal antibody and goat anti-mouse agarose. Immunoblots were incubated with a biotinylated anti-phosphotyrosine monoclonal antibody (Catalog # BAM1676) to detect phospho-MSP R. Bands were visualized with Streptavidin-HRP (Catalog # DY998) followed by chemiluminescent detection. Human phospho-MSP R can be detected in this DuoSet® IC ELISA by using approximately 6-12 times less lysate than is needed for a conventional IP-Western Blot." class="big_lightbox" target="_blank">
358-MT), human IGF-I sR (Catalog # 391-GR), or human EGF R (Catalog # 1095-ER) were added to 100 μg lysate and analyzed using this DuoSet® IC ELISA. Competition was observed only with recombinant human MSP R." class="big_lightbox" target="_blank">
4306-MS) is immobilized at 0.5 µg/mL, 100 µL/well, the concentration of Biotinylated Recombinant Human MSPR/Ron His-tag Avi-tag that produces 50% of the optimal binding response is approximately 40-240 ng/mL." class="big_lightbox" target="_blank">
Ron, the tyrosine kinase receptor for the Macrophage-stimulating protein, is involved in cell dissociation, motility, and matrix invasion (1). Ron, a cDNA homologous to the hepatocyte growth factor (HGF) receptor gene (MET), encodes a putative tyrosine kinase. It has been shown that the Ron gene is expressed in several epithelial tissues as well as in granulocytes and monocytes. The major RON transcript is translated into a glycosylated single chain precursor, cleaved into a 185 kDa heterodimer (p185Ron) of 35 (alpha) and 150 kDa (beta) disulfide-linked chains, before exposure at the cell surface (2).
![Western Blot MSPR/Ron [p Tyr1238, p Tyr1239, p Tyr1239] Antibody [Unconjugated]](http://images.novusbio.com/images/antibody/MSP_R_AF1947_Western_Blot_5160.jpg)
![Western Blot MSPR/Ron Antibody [Unconjugated]](http://images.novusbio.com/images/antibody/MSP_R_AF691_Western_Blot_21422.jpg)
![N/A MSPR/Ron [Biotin]](http://images.novusbio.com/images/dyc1947-2_human-phospho-msp-r-ron-duoset-ic-elisa-2-plate-712021212515.png)
![Binding Activity MSPR/Ron [Biotin]](http://images.novusbio.com/images/protein/MSPR_AVI1947_3115.jpg)
![Western Blot MSPR/Ron [p Tyr1238, p Tyr1239, p Tyr1239] Antibody [Unconjugated]](http://images.novusbio.com/images/antibody/MSP_R_AF1947_Immunoprecipitation_21469.jpg)
![Western Blot MSPR/Ron [p Tyr1238, p Tyr1239, p Tyr1239] Antibody [Unconjugated]](http://images.novusbio.com/fullsize/af1947_human-phospho-msp-r-ron-y1238-y1239-affinity-purified-pab-2282024173341.jpg)
![Western Blot MSPR/Ron [p Tyr1238, p Tyr1239, p Tyr1239] Antibody [Unconjugated]](http://images.novusbio.com/fullsize/af1947_human-phospho-msp-r-ron-y1238-y1239-affinity-purified-pab-1752025024030.jpg)
![Immunoprecipitation MSPR/Ron Antibody [Unconjugated]](http://images.novusbio.com/images/antibody/MSP_R_AF691_Immunoprecipitation_21423.jpg)
![N/A MSPR/Ron [Biotin]](http://images.novusbio.com/images/dyc1947-2_human-phospho-msp-r-ron-duoset-ic-elisa-2-plate-712021212822.png)
![N/A MSPR/Ron [Biotin]](http://images.novusbio.com/images/dyc1947-2_human-phospho-msp-r-ron-duoset-ic-elisa-2-plate-712021213417.png)
![SDS-Page MSPR/Ron [Biotin]](http://images.novusbio.com/fullsize/avi1947_recombinant-human-mspr-ron-his-tag-avi-tag-protein-cf-sds-page-18102022153036.jpg)
