Recombinant Human/Mouse Wnt-5a Protein, CF


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Product Details

Reactivity Hu, MuSpecies Glossary
Applications BA

Order Details

Recombinant Human/Mouse Wnt-5a Protein, CF Summary

Details of Functionality
Measured by its ability to inhibit Wnt-3a-induced alkaline phosphatase production by MC3T3‑E1 mouse preosteoblast cells. The ED50 for this effect is 0.1-0.5 µg/mL, in the presence of 5 ng/mL rmWnt-3a.
Optimal concentrations should be determined by each laboratory for each application.
Chinese Hamster Ovary cell line, CHO-derived
Accession #
N-terminal Sequence
Asn44 & No results obtained: Gln38 predicted
Protein/Peptide Type
Recombinant Proteins
>80%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.


Theoretical MW
38 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
45 kDa, reducing conditions
Read Publications using
645-WN/CF in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS, EDTA and CHAPS.
>80%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human/Mouse Wnt-5a Protein, CF

  • hWNT5A
  • protein Wnt-5a
  • wingless-type MMTV integration site family, member 5A
  • WNT-5A protein
  • Wnt5a
  • Wnt-5a


Wnt-5a is a 44‑50 kDa member of the Wnt family of proteins (1‑6). Based on its activity towards C57Mg mammary epithelium, it is classified as a nontransforming Wnt. Human Wnt‑5a is synthesized as a 380 amino acid (aa) precursor that contains a 37 aa signal sequence, a 25 aa prosegment, and a 319 aa mature region (1, 2, 3). The mature region has 24 cysteine residues that form multiple intrachain disulfide bonds, plus four N‑linked glycosylation sites that are utilized for proper secretion (3, 5, 7). There is also a palmitate adduct at Cys104 that is essential for activity, and a potential palmitoleic acid modification at Ser244 that may also contribute to secretion (7‑9). One alternative start site is reported at Met16. Over aa 38‑380, human and mouse Wnt‑5a are identical in amino acid sequence (1, 10). Cells known to express Wnt‑5a include brainstem astrocytes (11), mammary epithelium (12), CD34+ primitive progenitor stem cells (13), chondrocytes (14), CD34- pericytes and vascular smooth muscle cells (15), plus mesenchymal cells at various sites (16, 17). There are multiple receptors for Wnt‑5a. These include Fzd-1, -2,
-3, -4, -5, and -7 (3, 18‑22), Ror2 (3), LRP6 (23), Ryk (24) and sFRP1 (25). All these molecules function within the context of a larger number of “co‑factors” that regulate signaling by the Wnts. Initially, it was suggested that there were three pathways for Wnt signaling; a beta -catenin-mediated canonical pathway, and two noncanonical pathways described as the Wnt/JNK (PCP) pathway and the Wnt/Ca++ pathway (26, 27). And it was assumed that various Wnts could be accommodated by these classifications. At present, it is now recognized that individual Wnts, through various combinations of receptor complex subunits, can have diverse effects, perhaps even within the same cell (3, 6, 27). Further complexity is introduced by the fact that Xenopus Wnt‑5a and Wnt‑11 are known to form bioactive heterodimers following Tyr sulfation (28). Thus, predicting the activity of Wnt‑5a, or any other Wnt, on any cell type will require substantial insight into the interaction between all the extracellular, cell surface and intracellular components of the Wnt signaling system.

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Publications for (79)

We have publications tested in 5 confirmed species: Human, Mouse, Rat, N/A, Zebrafish.

We have publications tested in 4 applications: Binding Assay, Bioassay, In Vivo, WB Ctrl.

Filter By Application
Binding Assay
In Vivo
WB Ctrl
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All Species
Showing Publications 1 - 10 of 79. Show All 79 Publications.
Publications using 645-WN/CF Applications Species
A Ndoye, A Budina-Kol, CH Kugel, MR Webster, A Kaur, R Behera, VW Rebecca, L Li, PA Brafford, Q Liu, YN Vashisht G, MA Davies, GB Mills, X Xu, H Wu, M Herlyn, MC Nicastri, JD Winkler, MS Soengas, RK Amaravadi, ME Murphy, AT Weeraratna ATG5 mediates a positive feedback loop between Wnt signaling and autophagy in melanoma Cancer Res., 2017;0(0):. 2017 [PMID: 28887323] (Bioassay, Human) Bioassay Human
MW Susman, EP Karuna, RC Kunz, TS Gujral, AV Cantú, SS Choi, BY Jong, K Okada, MK Scales, J Hum, LS Hu, MW Kirschner, R Nishinakam, S Yamada, DJ Laird, LE Jao, SP Gygi, ME Greenberg, HH Ho Kinesin superfamily protein Kif26b links Wnt5a-Ror signaling to the control of cell and tissue behaviors in vertebrates Elife, 2017;6(0):. 2017 [PMID: 28885975] (Bioassay, Mouse) Bioassay Mouse
J Petersen, SC Wright, D Rodríguez, P Matricon, N Lahav, A Vromen, A Friedler, J Strömqvist, S Wennmalm, J Carlsson, G Schulte Agonist-induced dimer dissociation as a macromolecular step in G protein-coupled receptor signaling Nat Commun, 2017;8(1):226. 2017 [PMID: 28790300] (Bioassay, Human) Bioassay Human
MMF Steinbusch, MMJ Caron, DAM Surtel, F Friedrich, E Lausch, GJM Pruijn, W Verhesen, BLM Schroen, LW van Rhijn, B Zabel, TJM Welting Expression of RMRP RNA is regulated in chondrocyte hypertrophy and determines chondrogenic differentiation Sci Rep, 2017;7(1):6440. 2017 [PMID: 28743979] (Bioassay, Mouse) Bioassay Mouse
P Paclíková, O Bernatík, TW Radaszkiew, V Bryja N-terminal part of Dishevelled DEP domain is required for Wnt/?-catenin signaling in mammalian cells Mol. Cell. Biol., 2017;0(0):. 2017 [PMID: 28674183] (Human) Human
K Strakova, P Matricon, C Yokota, E Arthofer, O Bernatik, D Rodriguez, E Arenas, J Carlsson, V Bryja, G Schulte The tyrosine Y250(2.39) in Frizzled 4 defines a conserved motif important for structural integrity of the receptor and recruitment of Disheveled Cell. Signal., 2017;38(0):85-96. 2017 [PMID: 28668722] (Bioassay, Human) Bioassay Human
I Savvidou, T Khong, A Cuddihy, C McLean, S Horrigan, A Spencer Beta-catenin inhibitor BC2059 is efficacious as monotherapy or in combination with proteasome inhibitor bortezomib in multiple myeloma Mol. Cancer Ther., 2017;0(0):. 2017 [PMID: 28500235] (Bioassay, Human) Bioassay Human
Y Gao, A Cai, H Xi, J Li, W Xu, Y Zhang, K Zhang, J Cui, X Wu, B Wei, L Chen Ring finger protein 43 associates with gastric cancer progression and attenuates the stemness of gastric cancer stem-like cells via the Wnt-?/catenin signaling pathway Stem Cell Res Ther, 2017;8(1):98. 2017 [PMID: 28446252] (Bioassay, Human) Bioassay Human
M Vesel, J Rapp, D Feller, E Kiss, L Jaromi, M Meggyes, G Miskei, B Duga, G Smuk, T Laszlo, I Karner, JE Pongracz ABCB1 and ABCG2 drug transporters are differentially expressed in non-small cell lung cancers (NSCLC) and expression is modified by cisplatin treatment via altered Wnt signaling Respir. Res, 2017;18(1):52. 2017 [PMID: 28340578] (Bioassay, Human) Bioassay Human
K Kessenbroc, P Smith, SC Steenbeek, N Pervolarak, R Kumar, Y Minami, A Goga, L Hinck, Z Werb Diverse regulation of mammary epithelial growth and branching morphogenesis through noncanonical Wnt signaling Proc. Natl. Acad. Sci. U.S.A, 2017;0(0):. 2017 [PMID: 28270600] (Bioassay, Mouse) Bioassay Mouse
Show All 79 Publications.

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Gene Symbol WNT5A