Recombinant Human/Mouse Wnt-5a Protein, CF

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Recombinant mouse Wnt-5a (645-WN/CF) inhibits Wnt-3a-induced alkaline phosphatase production in the MC3T3-E1 mouse preosteoblast cell line. The ED50 for this effect is 0.1-0.5 µg/mL in the presence of 5 ng/mL ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

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Catalog# & Formulation Size Price

Recombinant Human/Mouse Wnt-5a Protein, CF Summary

Details of Functionality
Measured by its ability to inhibit Wnt-3a-induced alkaline phosphatase production by MC3T3‑E1 mouse preosteoblast cells. The ED50 for this effect is 0.1-0.5 µg/mL, in the presence of 5 ng/mL rmWnt-3a.
Optimal concentrations should be determined by each laboratory for each application.
Source
Chinese Hamster Ovary cell line, CHO-derived Wnt-5a protein
Gln38-Lys380
Accession #
N-terminal Sequence
Asn44 & No results obtained: Gln38 predicted
Protein/Peptide Type
Recombinant Proteins
Gene
WNT5A
Purity
>80%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
38 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
45 kDa, reducing conditions
Publications
Read Publications using
645-WN/CF in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS, EDTA and CHAPS.
Purity
>80%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human/Mouse Wnt-5a Protein, CF

  • hWNT5A
  • protein Wnt-5a
  • wingless-type MMTV integration site family, member 5A
  • WNT-5A protein
  • Wnt5a
  • Wnt-5a

Background

Wnt-5a is a 44‑50 kDa member of the Wnt family of proteins (1‑6). Based on its activity towards C57Mg mammary epithelium, it is classified as a nontransforming Wnt. Human Wnt‑5a is synthesized as a 380 amino acid (aa) precursor that contains a 37 aa signal sequence, a 25 aa prosegment, and a 319 aa mature region (1, 2, 3). The mature region has 24 cysteine residues that form multiple intrachain disulfide bonds, plus four N‑linked glycosylation sites that are utilized for proper secretion (3, 5, 7). There is also a palmitate adduct at Cys104 that is essential for activity, and a potential palmitoleic acid modification at Ser244 that may also contribute to secretion (7‑9). One alternative start site is reported at Met16. Over aa 38‑380, human and mouse Wnt‑5a are identical in amino acid sequence (1, 10). Cells known to express Wnt‑5a include brainstem astrocytes (11), mammary epithelium (12), CD34+ primitive progenitor stem cells (13), chondrocytes (14), CD34- pericytes and vascular smooth muscle cells (15), plus mesenchymal cells at various sites (16, 17). There are multiple receptors for Wnt‑5a. These include Fzd-1, -2,
-3, -4, -5, and -7 (3, 18‑22), Ror2 (3), LRP6 (23), Ryk (24) and sFRP1 (25). All these molecules function within the context of a larger number of “co‑factors” that regulate signaling by the Wnts. Initially, it was suggested that there were three pathways for Wnt signaling; a beta -catenin-mediated canonical pathway, and two noncanonical pathways described as the Wnt/JNK (PCP) pathway and the Wnt/Ca++ pathway (26, 27). And it was assumed that various Wnts could be accommodated by these classifications. At present, it is now recognized that individual Wnts, through various combinations of receptor complex subunits, can have diverse effects, perhaps even within the same cell (3, 6, 27). Further complexity is introduced by the fact that Xenopus Wnt‑5a and Wnt‑11 are known to form bioactive heterodimers following Tyr sulfation (28). Thus, predicting the activity of Wnt‑5a, or any other Wnt, on any cell type will require substantial insight into the interaction between all the extracellular, cell surface and intracellular components of the Wnt signaling system.

  1. Clark, C.C. et al. (1993) Genomics 18:249.
  2. LeJeune, S. et al. (1995) Clin. Cancer Res. 1:215.
  3. Mikels, A.J. & R. Nusse (2006) PLoS Biol. 4:e115.
  4. Nishita, M. et al. (2010) Trends Cell Biol. 20:346.
  5. Mikels, A.J. & R. Nusse (2006) Oncogene 25:7461.
  6. van Amerongen, R. & R. Nusse (2009) Development 136:3205.
  7. Kurayoshi, M. et al. (2007) Biochem. J. 402:515.
  8. Takada, R. et al. (2006) Dev. Cell 11:791.
  9. Port, F. & K. Basler (2010) Traffic May 3. [Epub ahead of print].
  10. Gavin, B.J. et al. (1990) Genes Dev. 4:2319.
  11. Castelo-Branco, G. et al. (2006) Mol. Cell. Neurosci. 31:251.
  12. Jonsson, M. et al. (1998) Br. J. Cancer 78:430.
  13. van Den Berg, D.J. et al. (1998) Blood 92:3189.
  14. Kruger, C. & C. Kappen (2010) PLoS One 5:e8978.
  15. Lin, G. et al. (2008) Stem Cells Dev. 17:1053.
  16. Lickert, H. et al. (2001) Mech. Dev. 105:181.
  17. Danielson, K.G. et al. (1995) J. Biol. Chem. 270:31225.
  18. Gazit, A. et al. (1999) Oncogene 18:5959.
  19. Bazhin, A. V. et al. (2010) Cell. Mol. Life Sci. 67:817.
  20. Kawasaki, A. et al. (2007) Cell. Signal. 19:2498.
  21. Blumenthal, A. et al. (2006) Blood 108:965.
  22. Umbhauer, M. et al. (2000) EMBO J. 19:4944.
  23. Bryja, V. et al. (2009) Mol. Biol. Cell 20:924.
  24. Keeble, T.R. et al. (2006) J. Neurosci. 26:5840.
  25. Lin, K. et al. (1997) Proc. Natl. Acad. Sci. USA 94:11196.
  26. Rao, T.P. & M. Kuhl (2010) Circ. Res. 106:1798.
  27. McDonald, S.L. & A. Silver (2009) Br. J. Cancer 101:209.
  28. Cha, S-W. et al. (2009) Curr. Biol. 19:1573.

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Publications for Wnt-5a (645-WN/CF)(161)

We have publications tested in 7 confirmed species: Human, Mouse, Rat, Bovine, N/A, Transgenic Mouse, Zebrafish.

We have publications tested in 9 applications: Binding Assay, Bioassay, Cell Culture, Control, Differentiation, ELISA Standard, In Vivo, Tissue Culture, Western Blot.


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Binding Assay
(1)
Bioassay
(132)
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(11)
Control
(1)
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(2)
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(1)
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(4)
Tissue Culture
(1)
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(1)
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Human
(97)
Mouse
(46)
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(8)
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(1)
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(2)
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(1)
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Showing Publications 1 - 10 of 161. Show All 161 Publications.
Publications using 645-WN/CF Applications Species
N Salerno, F Marino, M Scalise, L Salerno, C Molinaro, A Filardo, A Chiefalo, G Panuccio, A De Angelis, K Urbanek, D Torella, E Cianflone Pharmacological clearance of senescent cells improves cardiac remodeling and function after myocardial infarction in female aged mice Mechanisms of Ageing and Development, 2022;208(0):111740. 2022 [PMID: 36150603] (Differentiation, Mouse) Differentiation Mouse
X Xue, X Li, J Yao, X Zhang, X Ren, S Xu Transient and Prolonged Activation of Wnt Signaling Contribute Oppositely to the Pathogenesis of Asherman&#039;s Syndrome International Journal of Molecular Sciences, 2022;23(15):. 2022 [PMID: 35955940] (Cell Culture, Mouse) Cell Culture Mouse
C Kornsuthis, A Chansaenro, J Manokawinc, KA Tompkins, N Pirarat, T Osathanon Non-canonical Wnt signaling participates in Jagged1-induced osteo/odontogenic differentiation in human dental pulp stem cells Scientific Reports, 2022;12(1):7583. 2022 [PMID: 35534526] (Cell Culture, Human) Cell Culture Human
EM Ghia, LZ Rassenti, MY Choi, M Quijada-Ál, E Chu, GF Widhopf, TJ Kipps High expression level of ROR1 and ROR1-signaling associates with venetoclax resistance in chronic lymphocytic leukemia Leukemia, 2022;0(0):. 2022 [PMID: 35418613] (Cell Culture, Human) Cell Culture Human
CJ Brereton, L Yao, ER Davies, Y Zhou, M Vukmirovic, JA Bell, S Wang, RA Ridley, LSN Dean, OG Andriotis, F Conforti, L Brewitz, S Mohammed, T Wallis, A Tavassoli, RM Ewing, A Alzetani, BG Marshall, SV Fletcher, PJ Thurner, A Fabre, N Kaminski, L Richeldi, A Bhaskar, CJ Schofield, M Loxham, DE Davies, Y Wang, MG Jones Pseudohypoxic HIF pathway activation dysregulates collagen structure-function in human lung fibrosis Elife, 2022;11(0):. 2022 [PMID: 35188460] (Bioassay, Mouse) Bioassay Mouse
Z Dehghani-G, S Sheikh Has, E Arefian, G Hossein Wnt5A and TGFbeta1 Converges through YAP1 Activity and Integrin Alpha v Up-Regulation Promoting Epithelial to Mesenchymal Transition in Ovarian Cancer Cells and Mesothelial Cell Activation Cells, 2022;11(2):. 2022 [PMID: 35053353] (Bioassay, Human) Bioassay Human
Y Zhao, J He, T Zhu, Y Zhang, Y Zhai, P Xue, Y Yao, Z Zhou, M He, W Qu, Y Zhang Cadmium exposure reprograms energy metabolism of hematopoietic stem cells to promote myelopoiesis at the expense of lymphopoiesis in mice Oncogene, 2022;231(0):113208. 2022 [PMID: 35051759] (Bioassay, Mouse) Bioassay Mouse
J Neuhaus, A Weimann, M Berndt-Pae Immunocytochemical Analysis of Endogenous Frizzled-(Co-)Receptor Interactions and Rapid Wnt Pathway Activation in Mammalian Cells International Journal of Molecular Sciences, 2021;22(21):. 2021 [PMID: 34769487] (Bioassay, Human) Bioassay Human
M Kowalski-J, H Schihada, A Turku, T Huber, TP Sakmar, G Schulte Frizzled BRET sensors based on bioorthogonal labeling of unnatural amino acids reveal WNT-induced dynamics of the cysteine-rich domain Science Advances, 2021;7(46):eabj7917. 2021 [PMID: 34757789] (Bioassay, Human) Bioassay Human
T Radaszkiew, M Nosková, K Gömöryová, O Vondálová, KA Radaszkiew, M Picková, R Víchová, T Gybe?, K Kaiser, L Demková, L Ku?erová, T Bárta, D Pot?šil, Z Zdráhal, K Sou?ek, V Bryja RNF43 inhibits WNT5A-driven signaling and suppresses melanoma invasion and resistance to the targeted therapy Elife, 2021;10(0):. 2021 [PMID: 34702444] (Bioassay, Human) Bioassay Human
Show All 161 Publications.

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Bioinformatics

Gene Symbol WNT5A
Uniprot