Recombinant Human Meprin beta Subunit/MEP1B Protein, CF

Images

 
There are currently no images for Meprin beta Subunit/MEP1B (2895-ZN).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Enzyme Activity
Format
Carrier-Free

Order Details

Recombinant Human Meprin beta Subunit/MEP1B Protein, CF Summary

Details of Functionality
Measured by its ability to cleave a fluorogenic peptide substrate, Mca-SEVNLDAEFRK(Dpn)RR-NH2 (Catalog # ES004). The specific activity is >350 pmol/min/µg, as measured under the described conditions.
Source
Mouse myeloma cell line, NS0-derived human Meprin beta Subunit/MEP1B protein
Leu21-Ser593 & Thr23-Ser593, both with a C-terminal 10-His tag
Accession #
N-terminal Sequence
Leu21 & Thr23
Structure / Form
Pro form
Protein/Peptide Type
Recombinant Enzymes
Gene
MEP1B
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
66 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
85 kDa, reducing conditions
Publications
Read Publications using
2895-ZN in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
Buffer
Supplied as a 0.2 μm filtered solution in Tris, NaCl and Glycerol.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Assay Procedure
  • Activation Buffer: 50 mM Tris, 10 mM CaCl2, 150 mM NaCl, 0.05% (w/v) Brij-35, pH 7.5 (TCNB)
  • Assay Buffer: 50 mM Tris, pH 8.0
  • Recombinant Human Meprin  beta  Subunit/MEP1B (rhMEP1B) (Catalog # 2895-ZN)
  • Trypsin (Sigma, Catalog # T-1426 )
  • AEBSF (Catalog # EI001), 100 mM stock in water
  • Substrate: MCA-Ser-Glu-Val-Asn-Leu-Asp-Ala-Glu-Phe-Arg-Lys(DNP)-Arg-Arg-NH2 (Catalog # ES004)
  • F16 Black Maxisorp Plate (Nunc, Catalog # 475515)
  • Fluorescent Plate Reader (Model: SpectraMax Gemini EM by Molecular Devices) or equivalent
  1. Dilute rhMEP1B to 100 µg/mL with 1 µg/mL Trypsin in Activation Buffer.
  2. Incubate for 45 minutes at 37 °C. Stop Trypsin activation by adding AEBSF at a final concentration of 1 mM in Activation Buffer and mixing well.
  3. Dilute activated rhMEP1B to 0.4 µg/mL in Assay Buffer.
  4. Dilute Substrate to 20 µM in Assay Buffer.
  5. Load 50 µL of 0.4 µg/mL rhMEP1B into plate, and start the reaction by adding 50 µL of 20 µM Substrate. Include a Substrate Blank containing 50 µL of Assay Buffer and 50 µL of Substrate.
  6. Read at excitation and emission wavelengths of 320 nm and 405 nm (top read), respectively, in kinetic mode for 5 minutes.
  7. Calculate specific activity:

     Specific Activity (pmol/min/µg) =

Adjusted Vmax* (RFU/min) x Conversion Factor** (pmol/RFU)
amount of enzyme (µg)

     *Adjusted for Substrate Blank
     **Derived using calibration standard MCA-Pro-Leu-OH (Bachem, Catalog # M-1975).

Per Well:
  • rhMEP1B: 0.020 µg
  • Substrate: 10 µM

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Meprin beta Subunit/MEP1B Protein, CF

  • EC 3.4.24.63
  • endopeptidase-2
  • MEP1B
  • meprin A subunit beta
  • meprin A, beta
  • Meprin B
  • Meprin beta Subunit
  • N-benzoyl-L-tyrosyl-p-amino-benzoic acid hydrolase beta subunit
  • N-benzoyl-L-tyrosyl-P-amino-benzoic acid hydrolase subunit beta
  • PABA peptide hydrolase
  • PPH beta

Background

Meprins are multimeric proteases composed of alpha and beta subunits, which are members of the astacin family of zinc endopeptidases (1, 2). Both subunits form disulfide‑linked homo- or heterooligomers, which are also referred to as Merpin A (composed of alpha subunits with or without beta subunits) and Merpin B (composed of beta subunits only) (3). Although the two subunits share 42% identity in their amino acid sequence, they differ significantly in their oligomeric structure, post-translational processing and subsequently cellular location, and substrate and peptide bond specificity (4). The 701 amino acid sequence of human Merpin beta subunit precursor consists of a signal peptide (residues 1 to 21), a pro region (residues 22 to 61), and a mature chain (residues 62 to 701) containing the following domains, catalytic (residues 62 to 259), MAM (residues 260 to 429), MATH (residues 430 to 585), EGF-like (residues 604 to 644), transmembrane (residues 653 to 673), and cytoplasmic (residues 674 to 701). The pro enzyme terminating at residue 593 was expressed and the secreted protein purified from conditioned medium. After trypsin treatment, the activated enzyme cleaved a flurogenic peptide, which contains Asp and Glu, the preferred residues found in the P1’ and P1 sites (3).

  1. Bond, J.S. and R.J. Beynon (1995) Protein Sci. 4:1247.
  2. Stocker, W. et al. (1995) Protein Sci. 4:823.
  3. Bertenshaw, G.P. et al. (2001) J. Biol. Chem. 276:13248.
  4. Ishmael, F.T. et al. (2005) J. Biol. Chem. 280:13895.

Customers Who Viewed This Item Also Viewed...

AF3220
Species: Hu
Applications: WB, IHC, IP, CyTOF-ready, ICFlow
AF1513
Species: Mu
Applications: WB, Simple Western, Flow, IHC, IP, CyTOF-ready
NBP2-92546
Species: Hu, Mu, Rt
Applications: WB, ICC/IF
NBP2-34260
Species: Hu, Mu, Po, Bv, Rb
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
NBP2-67717
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P
NBP2-33903
Species: Hu
Applications: IHC, IHC-P
NBP1-81838
Species: Hu, Mu
Applications: WB, IHC, IHC-P
NB600-930
Species: Hu, Rt
Applications: WB, ELISA
NBP1-46535
Species: Hu, Mu, Rt, Rb, Xp, Ze
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P, IHC-FrFl
NBP1-89993
Species: Hu
Applications: WB, IHC, IHC-P
NBP2-41187
Species: Hu
Applications: WB, ELISA, IF
7270-IL/CF
Species: Hu
NBP1-90824
Species: Hu
Applications: WB, ICC/IF
NBP2-92677
Species: Hu, Mu, Rt
Applications: WB, ICC/IF
NB100-56565
Species: Hu, Mu, Rt
Applications: WB, Simple Western, ICC/IF, IHC, IHC-Fr, IHC-P
2895-ZN
Species: Hu
Applications: Enzyme Activity

Publications for Meprin beta Subunit/MEP1B (2895-ZN)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: Bioassay.


Filter By Application
Bioassay
(2)
All Applications
Filter By Species
Human
(2)
All Species

Reviews for Meprin beta Subunit/MEP1B (2895-ZN) (0)

There are no reviews for Meprin beta Subunit/MEP1B (2895-ZN). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

FAQs for Meprin beta Subunit/MEP1B (2895-ZN) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Other Available Formats

Additional Meprin beta Subunit/MEP1B Products

Bioinformatics Tool for Meprin beta Subunit/MEP1B (2895-ZN)

Discover related pathways, diseases and genes to Meprin beta Subunit/MEP1B (2895-ZN). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Meprin beta Subunit/MEP1B (2895-ZN)

Discover more about diseases related to Meprin beta Subunit/MEP1B (2895-ZN).
 

Pathways for Meprin beta Subunit/MEP1B (2895-ZN)

View related products by pathway.

PTMs for Meprin beta Subunit/MEP1B (2895-ZN)

Learn more about PTMs related to Meprin beta Subunit/MEP1B (2895-ZN).

Blogs on Meprin beta Subunit/MEP1B

There are no specific blogs for Meprin beta Subunit/MEP1B, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Recombinant Human Meprin beta Subunit/MEP1B Protein, CF and receive a gift card or discount.

Bioinformatics

Gene Symbol MEP1B
Uniprot