Recombinant Human LILRB1/CD85j/ILT2 Fc Chimera (Catalog #2017-T2) supports the adhesion of HSB2 humanperipheral blood acute lymphoblastic leukemia cells. Immobilized RecombinantHuman LILRB1/CD85j/ILT2 Fc Chimera at 5 ...read more
Recombinant Human LILRB1/CD85j/ILT2 Fc Chimera Protein, CF Summary
Details of Functionality
Measured by its ability to support the adhesion of HSB2 human peripheral blood acute lymphoblastic leukemia cells. Immobilized Recombinant Human LILRB1/CD85j/ILT2 Fc Chimera at 5 µg/mL, 100 µL/well can support 60-80% HSB2 cell adhesion, when 1 x 105 cells were added in each well of a 96-well plate.
Mouse myeloma cell line, NS0-derived human LILRB1/CD85j/ILT2 protein
Human LILRB1/CD85j/ILT2 (Gly24-His458) Accession # Q8NHL6
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
<0.10 EU per 1 μg of the protein by the LAL method.
73.8 kDa (monomer). Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
LILRB1, also known as CD85j and ILT2, is a 110 kDa transmembrane glycoprotein in the LILR immunoregulatory protein family (1). Mature human LILRB1 consists of a 438 amino acid (aa) extracellular domain (ECD) with 4 tandem Ig-like domains, a 21 aa transmembrane segment, and a 168 aa cytoplasmic domain with 4 inhibitory ITIM motifs (2). Alternative splicing generates an additional isoform that lacks the transmembrane and cytoplasmic regions (3). LILRB1 is expressed on the surface of B cells and monocytes, as well as subsets of NK cells, memory/effector CD8+ T cells, gamma δ T cells, and monocyte-derived dendritic cells (3-7). LILRB1 binds to MHC-I as well as non-classical MHC-I molecules (e.g. HLA-F, HLA-G, and HC-B27) and the MHC-I mimetic UL18 encoded by cytomegalovirus (3, 5, 8-10). R&D Systems in-house testing indicates that LILRB1 also binds to Angiopoietin-like 7. Ligation of LILRB1 inhibits the antigen induced proliferation and activation of CD8+ T cells, CD4+ T cells, NK cells, and gamma δ T cells (3, 4, 11-13). On dendritic cells, ligation inhibits the production of IL-10, IL-12p70, and TGF-beta and protects from Fas-mediated apoptosis (7).
Thomas, R. et al. (2010) Clin. Rev. Allergy Immunol. 38:159.
Samaridis, J. and M. Colonna (1997) Eur. J. Immunol. 27:660.
Colonna, M. et al. (1997) J. Exp. Med. 186:1809.
Harly, C. et al. (2011) Blood 117:2864.
Cosman, D. et al. (1997) Immunity 7:273.
Young, N.T. et al. (2001) J. Immunol. 166:3933.
Young, N.T. et al. (2008) Blood 111:3090.
Lepin, E.J.M. et al. (2000) Eur. J. Immunol. 30:3552.
Shiroishi, M. et al. (2003) Proc. Natl. Acad. Sci. USA 100:8856.
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
Review this Product
Be the first to review our Recombinant Human LILRB1/CD85j/ILT2 Fc Chimera Protein, CF and receive a gift card or discount.