Recombinant Human Langerin/CD207 Protein, CF

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Binding Activity
Format
Carrier-Free

Order Details

Recombinant Human Langerin/CD207 Protein, CF Summary

Details of Functionality
Measured by its ability to bind biotinylated Mannose-Polyacrylamide in a functional ELISA. Valladeau, J. et al. (2000) Immunity 12:71.
Source
Mouse myeloma cell line, NS0-derived human Langerin/CD207 protein
Tyr64-Pro328, with an N-terminal 9-His tag
Accession #
N-terminal Sequence
His
Protein/Peptide Type
Recombinant Proteins
Gene
CD207
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
31 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
35-40 kDa, reducing conditions
Publications
Read Publication using
2088-LN in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Langerin/CD207 Protein, CF

  • CD207 antigen
  • CD207 antigen, langerin
  • CD207 molecule, langerin
  • CD207
  • CLEC4KLangerhans cell specific c-type lectin
  • C-type lectin domain family 4 member K
  • C-type lectin domain family 4, member K
  • Langerin

Background

Langerin (also known as CD207) is a type II transmembrane glycoprotein which is member K of the C-type lectin domain family 4 (1). Langerin is used as a marker for Langerhans cells (LCs) which represent the immature dendritic cells in the epidermis (1, 2). LCs uniquely contain “tennis racket”-shaped endosomal recycling compartment subdomains with pentalamellar membranes termed Birbeck granules (1 - 3). Langerin is necessary and sufficient for Birbeck granule formation (1). The 328 amino acid (aa) human langerin sequence contains a 43 aa cytoplasmic domain, a 21 aa transmembrane domain and a 264 aa extracellular domain (ECD) that contains a coiled-coil domain and a single C-type lectin domain. Trimerization greatly increases the lectin binding affinity (4). Langerin internalizes endogenous proteins such as type I procollagen. Internalization by LC is thought to lead to suppression of self reactions (4 - 6). Langerin also mediates endocytosis of non-peptide antigens containing mannose, N-acetyl glucosamine and fucose that are expressed by mycobacteria and fungae (4, 7). Some antigens, such as the M. leprae glycolipid arabinomycolate, are ultimately presented by human LC CD1a in cutaneous-draining lymph nodes (8). Langerin performs a barrier-like function to HIV-1 transmission due to its internalization of virus particles for destruction (9). A rare human polymorphism within the lectin domain, W264R, abolishes both carbohydrate recognition and Birbeck granule formation (10, 11). Genetic deletion of mouse langerin was not shown to have functional consequence other than abolishing Birbeck granule formation (12). Human langerin shares 68%, 62%, 71% aa identity with mouse, rat and bovine langerin ECD, respectively.

  1. Valladeau, J. et al. (2000) Immunity 12:71. 
  2. Valladeau, J. et al. (2003) Immunol. Res. 28:93. 
  3. McDermott, R. et al. (2002) Mol. Biol. Cell 13:317. 
  4. Stambach, N. S. and M. E. Taylor (2003) Glycobiology 13:401. 
  5. Tada, Y. et al. (2006) J. Invest. Dermatol. 126:1549. 
  6. Ritter, U. and A. Osterloh (2007) Med. Microbiol. Immunol. 196:51. 
  7. Takahara, K. et al. (2003) Int. Immunol. 16:819.
  8. Hunger, R. E. et al. (2004) J. Clin. Invest. 113:701.
  9. De Witte, L. et al. (2007) Nat. Med. 13:367.
  10. Verdijk, P. et al. (2005) J. Invest. Dermatol. 124:714.
  11. Ward, E. M. et al. (2006) J. Biol. Chem. 281:15450.
  12. Kissenpfennig, A. et al. (2005) Mol. Cell. Biol. 25:88.

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Publications for Langerin/CD207 (2088-LN)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: Bioassay.


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Bioinformatics

Gene Symbol CD207
Uniprot