Recombinant Human Langerin/CD207 Protein, CF

• Reactivity: Hu
• Applications: Binding Activity
• Format: Carrier-Free

Recombinant Human Langerin/CD207 Protein, CF Summary

• Additional Information: His-tag
• Details of Functionality: Measured by its ability to bind biotinylated Mannose-Polyacrylamide in a functional ELISA. Valladeau, J. et al. (2000) Immunity 12:71.
• Source: Mouse myeloma cell line, NS0-derived human Langerin/CD207 protein
  Tyr64-Pro328, with an N-terminal 9-His tag
• Accession #: Q9UJ71
• N-terminal Sequence: His
• Protein/Peptide Type: Recombinant Proteins
• Gene: CD207
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Binding Activity
• Theoretical MW: 31 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 35-40 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Langerin/CD207 Protein, CF

• CD207 antigen
• CD207 antigen, langerin
• CD207 molecule, langerin
• CD207
• CLEC4KLangerhans cell specific c-type lectin
• C-type lectin domain family 4 member K
• C-type lectin domain family 4, member K
• Langerin

Background

Langerin (also known as CD207) is a type II transmembrane glycoprotein which is member K of the C-type lectin domain family 4 (1). Langerin is used as a marker for Langerhans cells (LCs) which represent the immature dendritic cells in the epidermis (1, 2). LCs uniquely contain “tennis racket”-shaped endosomal recycling compartment subdomains with pentalamellar membranes termed Birbeck granules (1 - 3). Langerin is necessary and sufficient for Birbeck granule formation (1). The 328 amino acid (aa) human langerin sequence contains a 43 aa cytoplasmic domain, a 21 aa transmembrane domain and a 264 aa extracellular domain (ECD) that contains a coiled-coil domain and a single C-type lectin domain. Trimerization greatly increases the lectin binding affinity (4). Langerin internalizes endogenous proteins such as type I procollagen. Internalization by LC is thought to lead to suppression of self reactions (4 - 6). Langerin also mediates endocytosis of non-peptide antigens containing mannose, N-acetyl glucosamine and fucose that are expressed by mycobacteria and fungae (4, 7). Some antigens, such as the M. leprae glycolipid arabinomycolate, are ultimately presented by human LC CD1a in cutaneous-draining lymph nodes (8). Langerin performs a barrier-like function to HIV-1 transmission due to its internalization of virus particles for destruction (9). A rare human polymorphism within the lectin domain, W264R, abolishes both carbohydrate recognition and Birbeck granule formation (10, 11). Genetic deletion of mouse langerin was not shown to have functional consequence other than abolishing Birbeck granule formation (12). Human langerin shares 68%, 62%, 71% aa identity with mouse, rat and bovine langerin ECD, respectively.

1. Valladeau, J. et al. (2000) Immunity 12:71. 
2. Valladeau, J. et al. (2003) Immunol. Res. 28:93. 
3. McDermott, R. et al. (2002) Mol. Biol. Cell 13:317. 
4. Stambach, N. S. and M. E. Taylor (2003) Glycobiology 13:401. 
5. Tada, Y. et al. (2006) J. Invest. Dermatol. 126:1549. 
6. Ritter, U. and A. Osterloh (2007) Med. Microbiol. Immunol. 196:51. 
7. Takahara, K. et al. (2003) Int. Immunol. 16:819.
8. Hunger, R. E. et al. (2004) J. Clin. Invest. 113:701.
9. De Witte, L. et al. (2007) Nat. Med. 13:367.
10. Verdijk, P. et al. (2005) J. Invest. Dermatol. 124:714.
11. Ward, E. M. et al. (2006) J. Biol. Chem. 281:15450.
12. Kissenpfennig, A. et al. (2005) Mol. Cell. Biol. 25:88.

Publications for Langerin/CD207 (2088-LN)(2)

Publications using 2088-LN

• C Gao, K Stavenhage, B Eckmair, TR McKitrick, AY Mehta, Y Matsumoto, AM McQuillan, MS Hanes, D Eris, KJ Baker, N Jia, M Wei, J Heimburg-M, B Ernst, RD Cummings Differential recognition of oligomannose isomers by glycan-binding proteins involved in innate and adaptive immunity Science Advances, 2021-06-09;7(24):. 2021-06-09 [PMID: 34108208] (Bioassay, Human)
• van den Berg L, Cardinaud S, van der Aar A, Sprokholt J, de Jong M, Zijlstra-Willems E, Moris A, Geijtenbeek T Langerhans Cell-Dendritic Cell Cross-Talk via Langerin and Hyaluronic Acid Mediates Antigen Transfer and Cross-Presentation of HIV-1. J Immunol, 2015-07-13;195(4):1763-73. 2015-07-13 [PMID: 26170391] (Bioassay, Human)

Bioinformatics

• Gene Symbol: CD207
• Uniprot:
  • Q9UJ71()