Recombinant Human L1CAM Fc Chimera Protein, CF

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

Recombinant Human L1CAM Fc Chimera Protein, CF Summary

Details of Functionality
Measured by the ability of the immobilized protein to support the adhesion of Neuro‑2A mouse neuroblastoma cells. When 5 x 104 cells/well are added to Recombinant Human L1CAM Fc Chimera coated plates (25 µg/mL with 100 µL/well), >30% will adhere for 1 hour incubation at 37 °C.
Optimal dilutions should be determined by each laboratory for each application.
Source
Mouse myeloma cell line, NS0-derived human L1CAM protein
Human L1CAM
(Ile20-Glu1120) & (Arg864‑Glu1120)
Accession # CAA42508
IEGRMD Human IgG1
(Pro100-Lys330)
6-His tag
N-terminus C-terminus
Accession #
N-terminal Sequence
Ile20 & Arg864
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
L1CAM
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
150.4 kDa (monomer) & 55.9 kDa (truncated).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
210-225 kDa, reducing conditions
Publications
Read Publications using
777-NC in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 4 weeks, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in Tris-Citrate and NaCl.
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 200 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human L1CAM Fc Chimera Protein, CF

  • antigen identified by monoclonal R1
  • CAML1
  • CAML1N-CAML1
  • CD171 antigen
  • CD171
  • HSAS
  • HSAS1
  • L1 cell adhesion molecule
  • L1CAM
  • MASA
  • MIC5
  • N-CAM-L1
  • NCAM-L1
  • neural cell adhesion molecule L1
  • S10
  • SPG1

Background

L1CAM (Neural cell adhesion molecule L1, also known as L1, CD171 and NCAM-L1) is a founding member of the L1 family, Immunoglobulin (Ig) superfamily of molecules (1-4). It was initially described as a 200-230 kDa neural adhesion molecule that likely played a key role in mouse nervous system development (4-6). Subsequent studies have confirmed the adhesive nature of the molecule, and expanded its activities in both neural and nonneural cell types. L1 is now recognized to play a key role in cell migration, adhesion, neurite outgrowth, myelination and neuronal differentiation (1, 7, 8). It does so through a series of cis and trans interactions that involve multiple copartners and target receptors (1, 3, 6, 8). Cells known to express L1 are varied, and include immature oligodendrocytes (9), CD4+ T cells, B cells and monocytes (10), both motor and sensory Schwann cells (11, 12), intestinal epithelial progenitor cells (12), cerebellar granule and Purkinje cells (5, 13, 14), and multiple tumor cells such as melanoma (15) plus pancreatic duct and lung carcinoma cells (16, 17). Human L1 was first identified as a 215 kDa glycoprotein on the surface of SKNAS neuroblastoma cells (18). Subsequent cloning established its precursor as being 1257 amino acids (aa) in length (19, 20). The molecule is a type I transmembrane (TM) protein that contains an 1101 aa extracellular region (aa 20-1120) plus a 114 aa cytoplasmic domain (aa 1144-1257). The extracellular region possesses six C2-type Ig-like domains (aa 35-607) followed by five fibronectin (FN) type III domains (aa 612-1108). As noted, L1 participates in multiple cis and trans interactions, and some of these interaction sites have been mapped to select Ig or FN domains. For instance, Ig-like domains #1, 2 and 6 associate with NP-1, L1 (homotypic binding), and various integrins, respectively (21‑23). The latter interaction is mediated by one (in human) or two (in mouse) RGD motifs (23, 24). Other molecules that heterotypically associate with L1 include NCAM, neurocan, CD24 and EGFR. The cytoplasmic tail contains no kinase motifs, but does possess a FIGQY peptide that interacts with ankyrin, and an RSLE sequence that mediates clathrin-associated endocytosis (1). There are two splice variants, one each in the intracellular and extracellular domains.  A deletion of RSLE adversely affects endocytosis, while a Leu substitution for aa 26-31 interfers with numerous heterotypic interactions (25, 26).  In general, the full-length L1 molecule is a neuron-associated isoform. L1 is known to undergo proteolysis, either by plasmin or ADAMs. This generates soluble isoforms of varying sizes (140-200 kDa) that retain bioactivity, and which can be incorporated into the surrounding ECM (5, 13, 27-30). The membrane fragments (30-80 kDa) undergo further processing, most importantly by gamma -secretase, to generate a soluble 28 kDa intracellular domain. This domain is SUMOylated, and believed to possess an NLS at Lys1147.  Upon presumed entry into the nucleus, L1 is posited to activate L1-responsive genes. Human and mouse L1 precursors share 88% aa sequence identity.
  1. Maness, P.F. and M. Schachner (2007) Nat. Neurosci. 10:19.
  2. Wei, C.H. and S.E. Ryu (2012) Exp. Mol. Med. 44:413.
  3. Faspel, J. and M. Grumet (2003) Front. Biosci. 8:1210.
  4. Herron, L.R. et al. (2009) Biochem. J. 419:519.
  5. Rathjen, F.G. and M. Schachner (1984) EMBO. J. 3:1.
  6. Keifel, H. et al. (2011) Trends Mol. Med. 17:178.
  7. Dihne, M. et al. (2003) J. Neurosci. 23:6638.
  8. Kadmon, G. et al. (1998) Dev. Immunol. 6:205.
  9. Itoh, K. et al. (2000) J. Neurosci. Res. 60:579.
  10. Jouet, M. et al. (1995) Mol. Brain Res. 30:378.
  11. He, Q. et al. (2012) Neurosci. Lett. 521:57.
  12. Thor, G. et al. (1987) EMBO J. 6:2581.
  13. Sadoul, K. et al. (1988) J. Neurochem. 50:510.
  14. Hubbe, M. et al. (1993) Eur. J. Immunol. 23:2927.
  15. Hoja-Lukowicz, D. et al. (2012) Glycoconj J. Apr 29. [Epub ahead of print].
  16. Geismann, C. et al. (2009) Cancer Res. 69:4517.
  17. Tischler, V. et al. (2011) Mol. Cancer 10:127.
  18. Mujoo, K. et al. (1986) J. Biol. Chem. 261:10299.
  19. Kobayashi, M. et al. (1991) Biochim. Biophys. Acta 1090:238.
  20. Hlavin, M.L. and V. Lemmon (1991) Genomics 11:416.
  21. Castellani, V. et al. (2002) EMBO J. 21:6348.
  22. Zhao, X. and C-H Siu (1995) J. Biol. Chem. 270:29413.
  23. Oleszewski, M. et al. (1999) J. Biol. Chem. 274:24602.
  24. Felding-Habermann, B. et al. (1997) J. Cell Biol. 139:1567.
  25. Kamiguchi, H. et al. (1998) J. Neurosci. 18:5311.
  26. De Angelis, E. et al. (2001) J. Biol. Chem. 276:32738.
  27. Montgomery, A.M.P. et al. (1996) J. Cell Biol. 132:475.
  28. Sadoul, R. et al. (1989) J. Neurochem. 53:1471.
  29. Reidle, S. et al. (2009) Biochem. J. 420:391.
  30. Lutz, D. et al. (2012) J. Biol. Chem. 287:17161.

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777-NC
Species: Hu
Applications: Bioactivity

Publications for L1CAM (777-NC)(4)

We have publications tested in 3 confirmed species: Human, Mouse, N/A.

We have publications tested in 2 applications: Bioassay, ELISA (Standard).


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Bioassay
(3)
ELISA (Standard)
(1)
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Human
(2)
Mouse
(1)
N/A
(1)
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Identifying tumoral and stromal transcriptomes that underlie tumor plasticity and stromal neuroinflammatory response in brain metastasis
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Bioinformatics

Gene Symbol L1CAM
Uniprot