Recombinant Human Kininostatin Protein

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity

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Recombinant Human Kininostatin Protein Summary

Details of Functionality
Measured by its ability to induce apoptosis of HUVEC human umbilical vein endothelial cells. Colman, R.W. et al. (2001) Arterioscler. Thromb. Vasc. Biol. 21:1427. The ED50 for this effect is 0.2-1 µg/mL in the presence of 10 ng/mL of rhFGF basic.
Source
E. coli-derived human Kininostatin protein
Lys438-Ser531
Accession #
N-terminal Sequence
Lys438
Protein/Peptide Type
Recombinant Proteins
Gene
KNG1
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
10.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
14 kDa, reducing conditions
Publications
Read Publication using
1396-KN in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 10 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Kininostatin Protein

  • Kininostatin

Background

High molecular weight kininogen (HK) is a secreted plasma glycoprotein consisting of six domains (D1 through D6). It binds specifically to endothelial cells and serves as a substrate of plasma kallikrein which releases bradykinin from within D4 of HK. Bradykinin is an active peptide that regulates multiple vascular processes. The resulting cleaved HK, called HKa, is a disulfide-linked dimer containing a heavy chain (D1 to D3) and a light chain (D5 and D6). HKa differs from HK in conformation and possesses new functional properties. It is a potent angiogenesis inhibitor that strongly inhibits endothelial cell proliferation and migration. HKa also induces apoptosis of endothelial cells. D5 of HKa has been shown to be the active region primarily responsible for the HKa anti-angiogenic activity and has been named kininostatin. While both HK and HKa are plasma proteins that are present in circulation, it is not known if kininostatin (D5) can be released from HKa and exist in plasma (1 - 4).

  1. Guo, Y. et al. (2002) International Immunopharmacology 2:1931.
  2. Zhang, J. et al. (2000) FASEB 14:2589.
  3. Colman, R.W. et al. (2000) Blood 95(2): 543.
  4. Guo, Y. et al. (2001) Arterioscler. Thromb. Vasc. Biol. 21:1427.

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Publications for Kininostatin (1396-KN)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: Binding Assay.


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Bioinformatics

Gene Symbol KNG1
Uniprot