Recombinant Human Intelectin-1/Omentin Protein, CF

When Streptococcus Pneumococcal Polysaccharide Type 70 is coated at 2 µg/mL (100 μL/well), Recombinant Human Intelectin-1/Omentin binds (Catalog # 9137-IN) with a typical ED50 of3-15 μg/mL.

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human Intelectin-1/Omentin Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. When Streptococcus Pneumococcal Polysaccharide Type 70 is coated at 2 µg/mL (100 μL/well), Recombinant Human Intelectin-1/Omentin binds with a typical ED₅₀ of 3-15 μg/mL.
• Source: Mouse myeloma cell line, NS0-derived human Intelectin-1/Omentin protein
  Trp17-Arg313
• Accession #: BAA96094
• N-terminal Sequence: Trp17 & Thr19
• Structure / Form: Disulfide-linked homotrimer
• Protein/Peptide Type: Recombinant Proteins
• Gene: Itln1
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 33 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 36-42 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in Tris.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 500 μg/mL in Water.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Intelectin-1/Omentin Protein, CF

• Endothelial lectin HL-1
• FLJ20022
• Galactofuranose-binding lectin
• hIntL
• HL-1
• intelectin 1 (galactofuranose binding)
• Intelectin1
• Intelectin-1
• INTLHL1
• ITLN1
• Itlna
• ITLNITLN-1
• LFR
• LFRIntestinal lactoferrin receptor
• Omentin
• Omentin-1

Background

Intelectin-1, also known as Omentin, HL-1, and Lactoferrin Receptor, is a multifunctional protein that plays a protective role in the response to cardiovascular injury (1). Mature human Intelectin-1 contains one fibrinogen C-terminal domain (2-4) and shares 82% and 80% aa sequence identity with mouse and rat Intelectin-1, respectively. Intelectin-1 is expressed by vascular smooth muscle and endothelial cells (3, 5, 6), particularly in the heart, small intestine, colon, thymus, ovary, testis, and omental fat (2, 3, 6) as well as by intestinal Paneth cells and airway and intestinal goblet cells (7-9). It is presented as a 35-40 kDa GPI-anchored glycoprotein that forms disulfide-linked homotrimers (2, 4) and can be released into the circulation and bronchiolar lavage fluid (6, 8). It binds to microbial galactofuranosyl carbohydrates (2, 11) and additionally functions as a receptor for the iron-transporting protein Lactoferrin (4). Following cardiovascular injury, Intelectin-1 limits fibrosis, cardiac hypertrophy, and carotid artery intimal hyperplasia (12, 13) foam cell generation [5], smooth muscle cell proliferation, migration, and calcification (5, 12, 14), and it also promotes revascularization (15). In addition, Intelectin-1 inhibits osteoblast bone matrix mineralization (14), enhances
insulin-stimulated uptake by adipocytes (6), supports neural stem cell viability (16), and inhibits the invasion and metastasis of neuroblastoma cells (17).

1. Jaikanth, C. et al. (2013) Exp. Clin. Endocrinol. Diabetes 121:377.
2. Tsuji, S. et al. (2001) J. Biol. Chem. 276:23456.
3. Lee, J.-K. et al. (2001) Glycobiology 11:65.
4. Suzuki, Y.A. et al. (2001) Biochemistry 40:15771.
5. Watanabe, K. et al. (2016) Cardiovasc. Res. PMID 26790473.
6. Yang, R.-Z. et al. (2006) Am. J. Physiol. Endocrinol. Metab. 290:E1253.
7. Komiya, T. et al. (1998) Biochem. Biophys. Res. Commun. 251:759.
8. Kerr, S.C. et al. (2014) Am. J. Respir. Crit. Care Med. 189:1005.
9. Washimi, K. et al. (2012) PLoS One 7:e39889.
10. Tsuji, S. et al. (2007) Glycobiology 17:1045.
11. Wesener, D.A. et al. (2015) Nat. Struct. Mol. Biol. 22:603.
12. Matsuo, K. et al. (2015) J. Mol. Cell. Cardiol. 79:195.
13. Kazama, K. et al. (2014) Am. J. Physiol. Heart Circ. Physiol. 306:H1714.
14. Xie, H. et al. (2011) Cardiovasc. Res. 92:296.
15. Maruyama, S. et al. (2012) J. Biol. Chem. 287:408.
16. Zhao, L.R. et al. (2015) Mol. Med. Rep. 11:1859.
17. Li, D. et al. (2015) Mol. Cancer 14:47.

Publications for Intelectin-1/Omentin (9137-IN)(2)

Publications using 9137-IN

• Seni?ar, M;Roubinet, B;Lafite, P;Legentil, L;Ferrières, V;Landemarre, L;Daniellou, R; Galf-Specific Neolectins: Towards Promising Diagnostic Tools International journal of molecular sciences 2024-04-28 [PMID: 38732045] (Functional Assay, Human)
• Xiao‐Ling Luo, Yun Jiang, Qiang Li, Xiu‐Jian Yu, Teng Ma, Hao Cao et al. hESC‐Derived Epicardial Cells Promote Repair of Infarcted Hearts in Mouse and Swine Advanced Science 2023-07-28 [PMID: 37505480] (Bioassay, Mouse)

Bioinformatics

• Gene Symbol: Itln1
• Uniprot:
  • BAA96094()