Reactivity | HuSpecies Glossary |
Applications | Bioactivity |
Format | Carrier-Free |
Details of Functionality | Measured by its binding ability in a functional ELISA. When Recombinant Human Vitronectin (Catalog # 2308-VN) is coated at 1 μg/mL, Recombinant Human Integrin alpha V beta 5 binds with an apparent KD <0.5 nM. |
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Source | Chinese Hamster Ovary cell line, CHO-derived human Integrin alpha V beta 5 protein
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Accession # | |||||||||||||||
N-terminal Sequence | Phe31 ( alpha V subunit) & Gly24 ( beta 5 subunit) |
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Structure / Form | Noncovalently-linked heterodimer |
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Protein/Peptide Type | Recombinant Proteins |
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Gene | ITGAV |
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Purity | >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining. |
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Endotoxin Note | <0.10 EU per 1 μg of the protein by the LAL method. |
Theoretical MW | 115.4 kDa ( alpha V subunit) & 84.8 kDa ( beta 5 subunit). Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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SDS-PAGE | 100-155 kDa, reducing conditions |
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Publications |
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Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Buffer | Lyophilized from a 0.2 μm filtered solution in PBS. |
Purity | >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining. |
Reconstitution Instructions | Reconstitute at 200 μg/mL in PBS. |
Integrin alpha V beta 5 is one of five alpha V integrins and the only known beta 5 integrin (1-3). The non-covalent heterodimer of 170 kDa alpha V and 100‑110 kDa beta 5 integrin type I transmembrane glycoprotein subunits is expressed on a wide variety of cell types including keratinocytes, fibroblasts, adhesive monocytes, embryonic stem cells, and select endothelium and epithelium (4‑8). alpha V beta 5 binds ligands containing an RGD motif, notably vitronectin (1‑10). The 962 aa human alpha V extracellular domain (ECD) shares 92-95% aa sequence identity with mouse, rat and cow alpha V, while the 696 aa human beta 5 ECD shares 89%-93% aa identity with mouse, rat, bovine, equine, and canine beta 5. The alpha V ECD contains an N-terminal beta - propeller structure, followed by domains termed thigh, calf-1 and calf-2 (1). The 799 aa beta 5 contains a vWFA domain within the ECD, which interacts with the alpha V beta -propeller to form a binding domain. Each subunit has a transmembrane sequence and a short cytoplasmic tail. Potential beta 5 isoforms include a 691 aa form with an alternate start site at aa 109, a 958 aa form with an alternate N‑terminus, and a 795 aa form with an alternate C‑terminus. Post-translational modifications, such as proteolytic cleavage of the alpha V subunit or phosphorylation of the beta 5 cytoplasmic tail, can increase endocytic turnover of the alpha V beta 5 protein and/or promote cell migration (7‑10). Growth factors that increase PKC activity, such as VEGF or TGF‑ alpha, promote alpha V beta 5-mediated angiogenesis while alpha V beta 3, which may be expressed in the same cell, responds to FGF-basic and TNF‑ alpha (11). An inhibitor of both down‑regulates tumor angiogenesis (12). During lung inflammation, up‑regulation of alpha V beta 5 on myofibroblasts or infiltrating lymphocytes may contribute to fibrosis by freeing TGF‑ beta from latency (13, 14). On retinal pigment epithelia, alpha V beta 5 is important for normal diurnal phagocytosis of outer rod segments, and contributes to adhesion of retinal cells (15).
Diseases for Integrin alpha V beta 5 (2528-AV)Discover more about diseases related to Integrin alpha V beta 5 (2528-AV).
| Pathways for Integrin alpha V beta 5 (2528-AV)View related products by pathway.
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PTMs for Integrin alpha V beta 5 (2528-AV)Learn more about PTMs related to Integrin alpha V beta 5 (2528-AV).
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Gene Symbol | ITGAV |
Uniprot |