Recombinant Human Integrin alpha V beta 5 Protein, CF

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

Recombinant Human Integrin alpha V beta 5 Protein, CF Summary

Details of Functionality
Measured by its binding ability in a functional ELISA. When Recombinant Human Vitronectin (Catalog # 2308-VN) is coated at 1 μg/mL, Recombinant Human Integrin  alpha V beta 5 binds with an apparent KD <0.5 nM.
Source
Chinese Hamster Ovary cell line, CHO-derived human Integrin alpha V beta 5 protein
Human Integrin alpha V
(Phe31-Val992)
Accession # P06756
His-Pro GGGSGGGS Acidic Tail 6-His tag
Human Integrin beta 5
(Gly24-Asn719)
Accession # P18084
His-Pro GGGSGGGS Basic Tail
N-terminus C-terminus
Accession #
N-terminal Sequence
Phe31 ( alpha V subunit) & Gly24 ( beta 5 subunit)
Structure / Form
Noncovalently-linked heterodimer
Protein/Peptide Type
Recombinant Proteins
Gene
ITGAV
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
115.4 kDa ( alpha V subunit) & 84.8 kDa ( beta 5 subunit).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
100-155 kDa, reducing conditions
Publications
Read Publications using
2528-AV in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 200 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Integrin alpha V beta 5 Protein, CF

  • CD51
  • Integrin alpha V beta 5
  • integrin subunit alpha V
  • ITGAV
  • MSK8
  • VNRA
  • VTNR

Background

Integrin alpha V beta 5 is one of five alpha V integrins and the only known beta 5 integrin (1-3). The non-covalent heterodimer of 170 kDa alpha V and 100‑110 kDa beta 5 integrin type I transmembrane glycoprotein subunits is expressed on a wide variety of cell types including keratinocytes, fibroblasts, adhesive monocytes, embryonic stem cells, and select endothelium and epithelium (4‑8). alpha V beta 5 binds ligands containing an RGD motif, notably vitronectin (1‑10). The 962 aa human alpha V extracellular domain (ECD) shares 92-95% aa sequence identity with mouse, rat and cow alpha V, while the 696 aa human beta 5 ECD shares 89%-93% aa identity with mouse, rat, bovine, equine, and canine beta 5. The alpha V ECD contains an N-terminal beta - propeller structure, followed by domains termed thigh, calf-1 and calf-2 (1). The 799 aa beta 5 contains a vWFA domain within the ECD, which interacts with the alpha V beta -propeller to form a binding domain. Each subunit has a transmembrane sequence and a short cytoplasmic tail. Potential beta 5 isoforms include a 691 aa form with an alternate start site at aa 109, a 958 aa form with an alternate N‑terminus, and a 795 aa form with an alternate C‑terminus. Post-translational modifications, such as proteolytic cleavage of the alpha V subunit or phosphorylation of the beta 5 cytoplasmic tail, can increase endocytic turnover of the alpha V beta 5 protein and/or promote cell migration (7‑10). Growth factors that increase PKC activity, such as VEGF or TGF‑ alpha, promote alpha V beta 5-mediated angiogenesis while alpha V beta 3, which may be expressed in the same cell, responds to FGF-basic and TNF‑ alpha (11). An inhibitor of both down‑regulates tumor angiogenesis (12). During lung inflammation, up‑regulation of alpha V beta 5 on myofibroblasts or infiltrating lymphocytes may contribute to fibrosis by freeing TGF‑ beta from latency (13, 14). On retinal pigment epithelia, alpha V beta 5 is important for normal diurnal phagocytosis of outer rod segments, and contributes to adhesion of retinal cells (15).

  1. Hynes, R. O. (2002) Cell 110:673.
  2. Suzuki, S. et al. (1987) J. Biol. Chem. 262:14080.
  3. Suzuki, S. et al. (1990) Proc. Natl. Acad. Sci. USA 87:5354.
  4. Smith, J.W. et al. (1990) J. Biol. Chem. 265:11008.
  5. Pasqualini, R. et al. (1993) J. Cell Sci. 105:101.
  6. Braam, S.R. et al. (2008) Stem Cells 26:2257.
  7. Memmo, L.M. and P. McKeown-Longo (1998) J. Cell Sci. 111:425.
  8. Kim, J.P. et al. (1994) J. Biol. Chem. 269:26926.
  9. Berthet, V. et al. (2004) FEBS Lett. 557:159.
  10. Li, Z. et al. (2010) Mol. Biol. Cell 21:3317.
  11. Friedlander, M. et al. (1995) Science 270:1500.
  12. Desgrosellier, J.S. and D.A. Cheresh (2010) Nat. Rev. Cancer 10:9.
  13. Wipff, P.J. et al. (2007) J. Cell Biol. 179:1311.
  14. Luzina, I.G. et al. (2009) Arthritis Rheum. 60:1530.
  15. Nandrot, E.F. et al. (2004) J. Exp. Med. 200:1539.

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Publications for Integrin alpha V beta 5 (2528-AV)(5)

We have publications tested in 2 confirmed species: Human, N/A.

We have publications tested in 4 applications: Binding Assay, Bioassay, ELISA Developmet, Surface Plasmon Resonance (SPR.


Filter By Application
Binding Assay
(2)
Bioassay
(1)
ELISA Developmet
(1)
Surface Plasmon Resonance (SPR
(1)
All Applications
Filter By Species
Human
(4)
N/A
(1)
All Species
Showing Publications 1 - 5 of 5.
Publications using 2528-AV Applications Species
SM Shin, JS Kim, SW Park, SY Jun, HJ Kweon, DK Choi, D Lee, YB Cho, YS Kim Direct targeting of oncogenic RAS mutants with a tumor-specific cytosol-penetrating antibody inhibits RAS mutant-driven tumor growth Sci Adv, 2020;6(3):eaay2174. 2020 [PMID: 31998840] (Bioassay, Human) Bioassay Human
S Bhatnagar, E Khera, J Liao, V Eniola, Y Hu, DE Smith, GM Thurber Oral and Subcutaneous Administration of a Near-Infrared Fluorescent Molecular Imaging Agent Detects Inflammation in a Mouse Model of Rheumatoid Arthritis Sci Rep, 2019;9(1):4661. 2019 [PMID: 30858419] (Binding Assay, Human) Binding Assay Human
KH Koh, Y Cao, S Mangos, NJ Tardi, RR Dande, HW Lee, B Samelko, MM Altintas, VP Schmitz, H Lee, K Mukherjee, V Peev, DJ Cimbaluk, J Reiser, E Hahm Nonimmune cell-derived ICOS ligand functions as a renoprotective alphavbeta3 integrin-selective antagonist J. Clin. Invest., 2019;129(4):1713-1726. 2019 [PMID: 30747722] (Surface Plasmon Resonance (SPR, Human) Surface Plasmon Resonance (SPR Human
Systematic site-directed mutagenesis of the Helicobacter pylori CagL protein of the Cag type IV secretion system identifies novel functional domains Sci Rep, 2016;6(0):38101. 2016 [PMID: 27922023] (ELISA Developmet, Human) ELISA Developmet Human
Kwon I, Hong S, Kim Y, Nam H, Kang S, Yang S, Heo J Thrombolytic effects of the snake venom disintegrin saxatilin determined by novel assessment methods: a FeCl3-induced thrombosis model in mice. PLoS ONE, 2013;8(11):e81165. 2013 [PMID: 24260554] (Binding Assay, N/A) Binding Assay N/A

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