Recombinant Human Integrin alpha V beta 5 Protein, CF

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human Integrin alpha V beta 5 Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. When Recombinant Human Vitronectin (Catalog # 2308-VN) is coated at 1 μg/mL, Recombinant Human Integrin alpha V beta 5 binds with an apparent K_D <0.5 nM.
• Source: Chinese Hamster Ovary cell line, CHO-derived human Integrin alpha V beta 5 protein
  

  Human Integrin alpha V (Phe31-Val992) Accession # P06756	His-Pro	GGGSGGGS	Acidic Tail	6-His tag
  Human Integrin beta 5 (Gly24-Asn719) Accession # P18084	His-Pro	GGGSGGGS	Basic Tail
  N-terminus				C-terminus

• Accession #: P06756 (Integrin alpha V) & P18084 (Integrin beta 5)
• N-terminal Sequence: Phe31 ( alpha V subunit) & Gly24 ( beta 5 subunit)
• Structure / Form: Noncovalently-linked heterodimer
• Protein/Peptide Type: Recombinant Proteins
• Gene: ITGAV
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Theoretical MW: 115.4 kDa ( alpha V subunit) & 84.8 kDa ( beta 5 subunit).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 100-155 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 200 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Integrin alpha V beta 5 Protein, CF

• CD51
• Integrin alpha V beta 5
• integrin subunit alpha V
• ITGAV
• MSK8
• VNRA
• VTNR

Background

Integrin alpha V beta 5 is one of five alpha V integrins and the only known beta 5 integrin (1-3). The non-covalent heterodimer of 170 kDa alpha V and 100‑110 kDa beta 5 integrin type I transmembrane glycoprotein subunits is expressed on a wide variety of cell types including keratinocytes, fibroblasts, adhesive monocytes, embryonic stem cells, and select endothelium and epithelium (4‑8). alpha V beta 5 binds ligands containing an RGD motif, notably vitronectin (1‑10). The 962 aa human alpha V extracellular domain (ECD) shares 92-95% aa sequence identity with mouse, rat and cow alpha V, while the 696 aa human beta 5 ECD shares 89%-93% aa identity with mouse, rat, bovine, equine, and canine beta 5. The alpha V ECD contains an N-terminal beta - propeller structure, followed by domains termed thigh, calf-1 and calf-2 (1). The 799 aa beta 5 contains a vWFA domain within the ECD, which interacts with the alpha V beta -propeller to form a binding domain. Each subunit has a transmembrane sequence and a short cytoplasmic tail. Potential beta 5 isoforms include a 691 aa form with an alternate start site at aa 109, a 958 aa form with an alternate N‑terminus, and a 795 aa form with an alternate C‑terminus. Post-translational modifications, such as proteolytic cleavage of the alpha V subunit or phosphorylation of the beta 5 cytoplasmic tail, can increase endocytic turnover of the alpha V beta 5 protein and/or promote cell migration (7‑10). Growth factors that increase PKC activity, such as VEGF or TGF‑ alpha, promote alpha V beta 5-mediated angiogenesis while alpha V beta 3, which may be expressed in the same cell, responds to FGF-basic and TNF‑ alpha (11). An inhibitor of both down‑regulates tumor angiogenesis (12). During lung inflammation, up‑regulation of alpha V beta 5 on myofibroblasts or infiltrating lymphocytes may contribute to fibrosis by freeing TGF‑ beta from latency (13, 14). On retinal pigment epithelia, alpha V beta 5 is important for normal diurnal phagocytosis of outer rod segments, and contributes to adhesion of retinal cells (15).

1. Hynes, R. O. (2002) Cell 110:673.
2. Suzuki, S. et al. (1987) J. Biol. Chem. 262:14080.
3. Suzuki, S. et al. (1990) Proc. Natl. Acad. Sci. USA 87:5354.
4. Smith, J.W. et al. (1990) J. Biol. Chem. 265:11008.
5. Pasqualini, R. et al. (1993) J. Cell Sci. 105:101.
6. Braam, S.R. et al. (2008) Stem Cells 26:2257.
7. Memmo, L.M. and P. McKeown-Longo (1998) J. Cell Sci. 111:425.
8. Kim, J.P. et al. (1994) J. Biol. Chem. 269:26926.
9. Berthet, V. et al. (2004) FEBS Lett. 557:159.
10. Li, Z. et al. (2010) Mol. Biol. Cell 21:3317.
11. Friedlander, M. et al. (1995) Science 270:1500.
12. Desgrosellier, J.S. and D.A. Cheresh (2010) Nat. Rev. Cancer 10:9.
13. Wipff, P.J. et al. (2007) J. Cell Biol. 179:1311.
14. Luzina, I.G. et al. (2009) Arthritis Rheum. 60:1530.
15. Nandrot, E.F. et al. (2004) J. Exp. Med. 200:1539.

Publications for Integrin alpha V beta 5 (2528-AV)(5)

Publications using 2528-AV

• SM Shin, JS Kim, SW Park, SY Jun, HJ Kweon, DK Choi, D Lee, YB Cho, YS Kim Direct targeting of oncogenic RAS mutants with a tumor-specific cytosol-penetrating antibody inhibits RAS mutant-driven tumor growth Sci Adv, 2020;6(3):eaay2174. 2020 [PMID: 31998840] (Bioassay, Human)
• S Bhatnagar, E Khera, J Liao, V Eniola, Y Hu, DE Smith, GM Thurber Oral and Subcutaneous Administration of a Near-Infrared Fluorescent Molecular Imaging Agent Detects Inflammation in a Mouse Model of Rheumatoid Arthritis Sci Rep, 2019;9(1):4661. 2019 [PMID: 30858419] (Binding Assay, Human)
• KH Koh, Y Cao, S Mangos, NJ Tardi, RR Dande, HW Lee, B Samelko, MM Altintas, VP Schmitz, H Lee, K Mukherjee, V Peev, DJ Cimbaluk, J Reiser, E Hahm Nonimmune cell-derived ICOS ligand functions as a renoprotective alphavbeta3 integrin-selective antagonist J. Clin. Invest., 2019;129(4):1713-1726. 2019 [PMID: 30747722] (Surface Plasmon Resonance (SPR, Human)
• Systematic site-directed mutagenesis of the Helicobacter pylori CagL protein of the Cag type IV secretion system identifies novel functional domains Sci Rep, 2016;6(0):38101. 2016 [PMID: 27922023] (ELISA Developmet, Human)
• Kwon I, Hong S, Kim Y, Nam H, Kang S, Yang S, Heo J Thrombolytic effects of the snake venom disintegrin saxatilin determined by novel assessment methods: a FeCl3-induced thrombosis model in mice. PLoS ONE, 2013;8(11):e81165. 2013 [PMID: 24260554] (Binding Assay, N/A)

Bioinformatics

• Gene Symbol: ITGAV
• Uniprot:
  • P06756 (Integrin alpha V) & P18084 (Integrin beta 5)()