Recombinant Human Integrin alpha V beta 5 Protein, CF

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Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

Recombinant Human Integrin alpha V beta 5 Protein, CF Summary

Details of Functionality
Measured by its binding ability in a functional ELISA. When Recombinant Human Vitronectin (Catalog # 2308-VN) is coated at 1 μg/mL, Recombinant Human Integrin  alpha V beta 5 binds with an apparent KD <0.5 nM.
Source
Chinese Hamster Ovary cell line, CHO-derived human Integrin alpha V beta 5 protein
Human Integrin alpha V
(Phe31-Val992)
Accession # P06756
His-Pro GGGSGGGS Acidic Tail 6-His tag
Human Integrin beta 5
(Gly24-Asn719)
Accession # P18084
His-Pro GGGSGGGS Basic Tail
N-terminus C-terminus
Accession #
N-terminal Sequence
Phe31 ( alpha V subunit) & Gly24 ( beta 5 subunit)
Structure / Form
Noncovalently-linked heterodimer
Protein/Peptide Type
Recombinant Proteins
Gene
ITGAV
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
115.4 kDa ( alpha V subunit) & 84.8 kDa ( beta 5 subunit).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
100-155 kDa, reducing conditions
Publications
Read Publications using
2528-AV in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 200 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Integrin alpha V beta 5 Protein, CF

  • CD51
  • Integrin alpha V beta 5
  • integrin subunit alpha V
  • ITGAV
  • MSK8
  • VNRA
  • VTNR

Background

Integrin alpha V beta 5 is one of five alpha V integrins and the only known beta 5 integrin (1-3). The non-covalent heterodimer of 170 kDa alpha V and 100‑110 kDa beta 5 integrin type I transmembrane glycoprotein subunits is expressed on a wide variety of cell types including keratinocytes, fibroblasts, adhesive monocytes, embryonic stem cells, and select endothelium and epithelium (4‑8). alpha V beta 5 binds ligands containing an RGD motif, notably vitronectin (1‑10). The 962 aa human alpha V extracellular domain (ECD) shares 92-95% aa sequence identity with mouse, rat and cow alpha V, while the 696 aa human beta 5 ECD shares 89%-93% aa identity with mouse, rat, bovine, equine, and canine beta 5. The alpha V ECD contains an N-terminal beta - propeller structure, followed by domains termed thigh, calf-1 and calf-2 (1). The 799 aa beta 5 contains a vWFA domain within the ECD, which interacts with the alpha V beta -propeller to form a binding domain. Each subunit has a transmembrane sequence and a short cytoplasmic tail. Potential beta 5 isoforms include a 691 aa form with an alternate start site at aa 109, a 958 aa form with an alternate N‑terminus, and a 795 aa form with an alternate C‑terminus. Post-translational modifications, such as proteolytic cleavage of the alpha V subunit or phosphorylation of the beta 5 cytoplasmic tail, can increase endocytic turnover of the alpha V beta 5 protein and/or promote cell migration (7‑10). Growth factors that increase PKC activity, such as VEGF or TGF‑ alpha, promote alpha V beta 5-mediated angiogenesis while alpha V beta 3, which may be expressed in the same cell, responds to FGF-basic and TNF‑ alpha (11). An inhibitor of both down‑regulates tumor angiogenesis (12). During lung inflammation, up‑regulation of alpha V beta 5 on myofibroblasts or infiltrating lymphocytes may contribute to fibrosis by freeing TGF‑ beta from latency (13, 14). On retinal pigment epithelia, alpha V beta 5 is important for normal diurnal phagocytosis of outer rod segments, and contributes to adhesion of retinal cells (15).

  1. Hynes, R. O. (2002) Cell 110:673.
  2. Suzuki, S. et al. (1987) J. Biol. Chem. 262:14080.
  3. Suzuki, S. et al. (1990) Proc. Natl. Acad. Sci. USA 87:5354.
  4. Smith, J.W. et al. (1990) J. Biol. Chem. 265:11008.
  5. Pasqualini, R. et al. (1993) J. Cell Sci. 105:101.
  6. Braam, S.R. et al. (2008) Stem Cells 26:2257.
  7. Memmo, L.M. and P. McKeown-Longo (1998) J. Cell Sci. 111:425.
  8. Kim, J.P. et al. (1994) J. Biol. Chem. 269:26926.
  9. Berthet, V. et al. (2004) FEBS Lett. 557:159.
  10. Li, Z. et al. (2010) Mol. Biol. Cell 21:3317.
  11. Friedlander, M. et al. (1995) Science 270:1500.
  12. Desgrosellier, J.S. and D.A. Cheresh (2010) Nat. Rev. Cancer 10:9.
  13. Wipff, P.J. et al. (2007) J. Cell Biol. 179:1311.
  14. Luzina, I.G. et al. (2009) Arthritis Rheum. 60:1530.
  15. Nandrot, E.F. et al. (2004) J. Exp. Med. 200:1539.

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Publications for Integrin alpha V beta 5 (2528-AV)(2)

We have publications tested in 2 confirmed species: Human, N/A.

We have publications tested in 2 applications: Binding Assay, ELISA development.


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(1)
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