Recombinant Human Integrin alpha 4 beta 1 Protein, CF

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

Recombinant Human Integrin alpha 4 beta 1 Protein, CF Summary

Details of Functionality
Measured by the ability of the immobilized protein to support the adhesion of CHO Chinese hamster ovary cells transfected with VCAM-1. When 5 x 104 cells per well are added to rhIntegrin alpha 4 beta 1 coated plates (10 μg/mL,
100 μL/well), approximately 45%-75% will adhere after 1 hour at 37 °C.
Source
Chinese Hamster Ovary cell line, CHO-derived human Integrin alpha 4 beta 1 protein
Human Integrin alpha 4
(Tyr34-Gln970 (Arg591Leu & Arg878Gln))
Accession # P13612
Acidic Tail 6-His tag
Human Integrin beta 1
(Gln21-Asp728)
Accession # P05556
Basic Tail
N-terminus C-terminus
Accession #
N-terminal Sequence
Tyr34 ( alpha 4) & No results obtained: Gln21predicted ( beta 1)
Protein/Peptide Type
Recombinant Proteins
Gene
ITGA4
Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
108.8 kDa ( alpha 4) & 82.3 kDa ( beta 1).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
100-150 kDa, reducing conditions
Publications
Read Publications using
5668-A4 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 200 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Integrin alpha 4 beta 1 Protein, CF

  • Integrin alpha 4 beta 1

Background

Integrin alpha 4 beta 1, also called VLA4, is an integrin family adhesion receptor that shares the beta 1 subunit with eleven other family members and the alpha 4 subunit with integrin alpha 4 beta 7 (1 - 4). The non-covalent heterodimer of 150 kDa alpha 4/CD49d and 130 kDa beta 1/CD29 type I transmembrane glycoprotein subunits mediates cell adhesion to VCAM-1/CD106 on other cells and the CS-1 fragment of fibronectin in the extracellular matrix (2 - 4). The alpha 4 extracellular domain (ECD) contains an N-terminal beta -propeller structure, followed by domains termed thigh, calf-1 and calf-2 (1). The beta 1 ECD contains a vWFA domain, which interacts with the alpha 4 beta -propeller to form a binding domain when the dimer is in active, extended and open conformation. Each subunit has a transmembrane sequence and a short cytoplasmic tail. The 944 aa human alpha 4 extracellular domain (ECD) shares 85% aa identity with mouse, rat and canine alpha 4, while the 708 aa human beta 1 ECD shares 92 - 96% aa identity with rat, bovine, mouse, and feline beta 1. Five alternate splice forms of the human beta 1 cytoplasmic domain, including one antagonistic form, vary by 12 to 48 aa and show differential expression patterns (5). Leukocytes (except for neutrophils), erythroid precursors and some non-hematopoietic cells such as epicardial, endothelial and smooth muscle precursors, Schwann cells, and chorionic cells express alpha 4 beta 1 (6 - 10). Deletion is lethal in the mouse embryo due to faulty placentation and development of the epicardium and coronary vessels (7, 10). In the adult, alpha 4 beta 1 primarily regulates immune cell migration (11 - 13). Circulating leukocyte alpha 4 beta 1 is rapidly activated by inflamed endothelial cells that present VCAM-1 and chemokines such as SDF-1 (11). This activation facilitates rolling, firm adhesion, and extravasation. Interfering with leukocyte migration via the therapeutic alpha 4 beta 1antibody Natalizumab can reduce the severity of autoimmune disorders such as multiple sclerosis (12). Natalizumab can also mobilize hematopoietic precursors from the bone marrow by impeding their interaction with stromal cell VCAM-1 (8, 12). During immune cell activation, alpha 4 beta 1 can function as a costimulatory molecule (13, 14).
  1. Luo, B-H. et al. (2007) Annu. Rev. Immunol. 25:619.
  2. Takada, Y. et al. (1989) EMBO J. 8:1361.
  3. Argraves, W.S. et al. (1987) J. Cell Biol. 1025:1183.
  4. Takada, Y. et al. (2007) Genome Biol. 8:215.
  5. Svineng, G. et al. (1998) Biochem. J. 330:1255.
  6. Eshghi, S. et al. (2007) J. Cell Biol. 177:871.
  7. Yang, J.T. et al. (1995) Development 121:549.
  8. Sheppard, A.M. et al. (1994) Cell Adhes. Commun. 2:27.
  9. Haack, H. and R.O. Hynes (2001) Dev. Biol. 233:38.
  10. Grazioli, A. et al. (2006) Dev. Biol. 293:165.
  11. Alon, R. and K. Ley (2008) Curr. Opin. Cell Biol. 20:525.
  12. Bauer, M. et al. (2009) Proc. Natl. Acad. Sci. USA 106:1920.
  13. Banerjee, E.R. et al. (2008) Exp. Hematol. 36:1004.
  14. Nguyen, K. et al. (2008) Immunity 28:810.

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Publications for Integrin alpha 4 beta 1 (5668-A4)(4)

We have publications tested in 2 confirmed species: Human, Equine.

We have publications tested in 2 applications: Bioassay, Surface Plasmon Resonance.


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Bioassay
(3)
Surface Plasmon Resonance
(1)
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(2)
Equine
(1)
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