Recombinant Human Integrin alpha 1 beta 1 Protein, CF

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human Integrin alpha 1 beta 1 Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. When Collagen I is immobilized at 5 µg/mL, Recombinant Human Integrin alpha 1 beta 1 can bind with an apparent K_D <5 nM.
  Optimal dilutions should be determined by each laboratory for each application.
• Source: Chinese Hamster Ovary cell line, CHO-derived human Integrin alpha 1 beta 1 protein
  

  Human Integrin alpha 1 (Met1-Pro1141) Accession # P56199	GGGSGGGSGGGS	Acidic Tail	HHHHHH
  Human Integrin beta 1 (Gln21-Asp728) Accession # P05556	GGGSGGGSGGGS	Basic Tail
  N-terminus			C-terminus

• Accession #: P56199 (Integrin alpha 1) & P05556 (Integrin beta 1)
• N-terminal Sequence: Phe29 (Integrin alpha 1) & No results obtained: Gln21 predicted (Integrin beta 1), sequencing might be blocked
• Protein/Peptide Type: Recombinant Proteins
• Gene: ITGA1
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Endotoxin Note: <1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 132.3 kDa (Integrin alpha 1) & 86.4 kDa (Integrin beta 1).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 180-200 & 110-130 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution Instructions: Reconstitute at 100 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Integrin alpha 1 beta 1 Protein, CF

• Integrin alpha 1 beta 1

Background

Integrin alpha 1 beta 1, also called VLA1 is the only alpha 1 integrin family adhesion receptor, one of twelve integrins that share the beta 1 subunit, and one of four collagen‑binding integrins (1‑5). It is the non‑covalent heterodimer of 190‑210 kDa alpha 1 (CD49a) and 130 kDa beta 1 (CD29) type I transmembrane glycoprotein subunits. It is found on cells including activated T cells, B cells, monocytes, vascular smooth muscle cells, osteoblasts and adipocytes (2, 6‑8). The alpha 1 extracellular domain (ECD) contains an I (inserted) domain which includes the ligand binding site (2, 4). The beta 1 ECD contains a vWFA domain, which participates in binding (3). Each subunit then has a transmembrane sequence and a short cytoplasmic tail. Divalent cations and intracellular (inside-out) signaling convert the dimer from the folded, inactive form to its most active, extended conformation (1, 2). The 1113 amino acid (aa) human alpha 1 extracellular domain (ECD) shares 88‑92% aa sequence identity with mouse, rat, canine, bovine and equine alpha 1, while the 708 aa human beta 1 ECD shares 92‑96% aa sequence identity with rat, bovine, mouse, and feline beta 1. Potential alpha 1 splice variants show two or four aa substitutions for aa 765‑1179. alpha 1 beta 1 preferentially binds collagens I, IV, VI, XIII and XVI, but also binds laminin (4‑10). alpha 1 beta 1 is reported to down‑regulate EGF R signaling, increase expression of caveolin-1, reduce production of reactive oxygen species, regulate collagen expression, control MMP collagenase and gelatinase activity, and mediate the renal basement membrane disorder Alport syndrome (10‑12). These effects may begin by alpha 1 beta 1 binding of caveolin-1, initiating signaling pathways that involve the phosphatase TC-PTP, kinases ERK and p38, and the transcription factor PPAR-gamma (10‑13). alpha 1 beta 1 down‑regulates MMP-mediated angiostatin formation, enhancing tumor vascularization (8). alpha 1 beta 1-null mice are deficient in fibroblast collagen IV and laminin-mediated cell spreading and migration, show defects in bone healing, and are resistant to Alport renal fibrosis (9‑11, 14). When expressed in the same epithelial cells, alpha 1 beta 1 negatively regulates integrin alpha 2 beta 1‑mediated cell adhesion and migration (15).

1. Takada, Y. et al. (2007) Genome Biol. 8:215.
2. Luo, B-H. et al. (2007) Annu. Rev. Immunol. 25:619.
3. Argraves, W.S. et al. (1987) J. Cell Biol. 105:1183.
4. Briesewitz, R. et al. (1993) J. Biol. Chem. 268:2989.
5. Tulla, M. et al. (2001) J. Biol. Chem. 276:48206.
6. Eble, J.A. et al. (2006) J. Biol. Chem. 281:25745.
7. Hall, D.E. et al. (1990) J. Cell Biol. 110:2175.
8. Pozzi, A. et al. (2000) Proc. Natl. Acad. Sci. USA 97:2202.
9. Gardner, H. et al. (1999) J. Cell Sci. 112:263.
10. Dennis, J. et al. (2010) Am. J. Pathol. 177:2527.
11. Cosgrove, D. et al. (2008) Am. J. Pathol. 172:761.
12. Chen, X. et al. (2010) Mol. Cell. Biol. 30:3048.
13. Borza, C.M. et al. (2010) J. Biol. Chem. 285:40114.
14. Gardner, H. et al. (1996) Dev. Biol. 175:301.
15. Abair, T.D. et al. (2008) Exp. Cell Res. 314:3593.

Publications for Integrin alpha 1 beta 1 (7064-AB)(2)

Publications using 7064-AB

• Stampolidis P, Ullrich A, Iacobelli S LGALS3BP, lectin galactoside-binding soluble 3 binding protein, promotes oncogenic cellular events impeded by antibody intervention. Oncogene, 2013-12-23;34(1):39-52. 2013-12-23 [PMID: 24362527] (Bioassay, Human)
• Kwon I, Hong S, Kim Y, Nam H, Kang S, Yang S, Heo J Thrombolytic effects of the snake venom disintegrin saxatilin determined by novel assessment methods: a FeCl3-induced thrombosis model in mice. PLoS ONE, 2013-11-18;8(11):e81165. 2013-11-18 [PMID: 24260554] (Binding Assay)

Bioinformatics

• Gene Symbol: ITGA1
• Uniprot:
  • P56199 (Integrin alpha 1) & P05556 (Integrin beta 1)()