Recombinant Human IL-6, Animal-Free Protein

Equivalent bioactivity with GMP, Animal-Free, and RUO grades of cytokines as measured in cell proliferation assays. GMP, Animal-Free, RUO grades of Human IL-6 (orange, green, red, respectively).

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human IL-6, Animal-Free Protein Summary

• Additional Information: Intended for preclinical researchers who may transition to GMP IL-6 for their clinical work
• Details of Functionality: Measured in a cell proliferation assay using T1165.85.2.1 mouse plasmacytoma cells. Nordan, R.P. et al. (1987) J. Immunol. 139:813. The ED₅₀ for this effect is 0.200‑0.800 ng/mL.
  The specific activity of Recombinant Human IL-6 is >1.00 x 10⁸ IU/mg, which is calibrated against the human IL‑6 WHO International Standard (NIBSC code: 89/548).
• Source: E. coli-derived human IL-6 protein
  Pro29-Met212
  Produced using non-animal reagents in an animal-free laboratory.
• Accession #: P05231.1
• N-terminal Sequence: Pro29
• Protein/Peptide Type: Animal-Free Recombinant Proteins
• Gene: IL6
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Purity Statement: Antigen Affinity-purified
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 20.9 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS and NaCl.
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human IL-6, Animal-Free Protein

• B-cell differentiation factor
• B-cell stimulatory factor 2
• BSF2
• BSF-2
• CDF
• CTL differentiation factor
• HSF
• hybridoma growth factor
• IFNB2
• IFN-beta-2
• IL6
• IL-6
• Interferon beta-2
• interleukin 6 (interferon, beta 2)
• interleukin BSF-2
• interleukin-6
• MGI-2A

Background

Interleukin-6 (IL-6) is a pleiotropic, alpha -helical, 22-28 kDa phosphorylated and variably glycosylated cytokine that plays important roles in the acute phase reaction, inflammation, hematopoiesis, bone metabolism, and cancer progression (1-5). Mature human IL-6 is 183 amino acids (aa) in length and shares 39% aa sequence identity with mouse and rat IL-6 (6). Alternative splicing generates several isoforms with internal deletions, some of which exhibit antagonistic properties (7-10). IL-6 induces signaling through a cell surface heterodimeric receptor complex composed of a ligand binding subunit (IL-6 R alpha) and a signal transducing subunit (gp130). IL-6 binds to IL-6 R alpha , triggering IL-6 R alpha association with gp130 and gp130 dimerization (11). gp130 is also a component of the receptors for CLC, CNTF, CT-1, IL-11, IL-27, LIF, and OSM (12). Soluble forms of IL-6 R alpha are generated by both alternative splicing and proteolytic cleavage (5). In a mechanism known as trans-signaling, complexes of soluble IL-6 and IL-6 R alpha elicit responses from gp130-expressing cells that lack cell surface IL-6 R alpha (5). Trans-signaling enables a wider range of cell types to respond to IL-6, as the expression of gp130 is ubiquitous, while that of IL-6 R alpha is predominantly restricted to hepatocytes, monocytes, and resting lymphocytes (2, 5). Soluble splice forms of gp130 block trans-signaling from IL-6/IL-6 R alpha but not from other cytokines that use gp130 as a co-receptor (5, 13). IL-6, along with TNF-alpha and IL-1, drives the acute inflammatory response and the transition from acute inflammation to either acquired immunity or chronic inflammatory disease (1-5). When dysregulated, it contributes to chronic inflammation in obesity, insulin resistance, inflammatory bowel disease, arthritis, sepsis, and atherosclerosis (1, 2, 5). IL-6 can also function as an anti-inflammatory molecule, as in skeletal muscle where it is secreted in response to exercise (2). In addition, it enhances hematopoietic stem cell proliferation and the differentiation of Th17 cells, memory B cells, and plasma cells (1, 14).

1. Mansell, A. and B.J. Jenkins (2013) Cytokine Growth Factor Rev. 24:249.
2. Schuett, H. et al. (2009) Thromb. Haemost. 102:215.
3. Erta, M. et al. (2012) Int. J. Biol. Sci. 8:1254.
4. Garbers, C. et al. (2012) Cytokine Growth Factor Rev. 23:85.
5. Mihara, M. et al. (2012) Clin. Sci. (Lond.) 122:143.
6. Hirano, T. et al. (1986) Nature 324:73.
7. Kestler, D.P. et al. (1995) Blood 86:4559.
8. Kestler, D.P. et al. (1999) Am. J. Hematol. 61:169.
9. Bihl, M.P. et al. (2002) Am. J. Respir. Cell Mol. Biol. 27:48.
10. Alberti, L. et al. (2005) Cancer Res. 65:2.
11. Murakami, M. et al. (1993) Science 260:1808.
12. Muller-Newen, G. (2003) Sci. STKE 2003:PE40.
13. Mitsuyama, K. et al. (2006) Clin. Exp. Immunol. 143:125.
14. Cerutti, A. et al. (1998) J. Immunol. 160:2145.

Manufacturing Process

Animal-Free Manufacturing Conditions
Our dedicated controlled-access animal-free laboratories ensure that at no point in production are the products exposed to potential contamination by animal components or byproducts. Every stage of manufacturing is conducted in compliance with R&D Systems' stringent Standard Operating Procedures (SOPs). Production and purification procedures use equipment and media that are confirmed animal-free.

Production

• All molecular biology procedures use animal-free media and dedicated labware.
• Dedicated fermentors are utilized in committed animal-free areas.

Purification

• Protein purification columns are animal-free.
• Bulk proteins are filtered using animal-free filters.
• Purified proteins are stored in animal-free containers in a dedicated cold storage room.

Quality Assurance

• Low Endotoxin Level.
• No impairment of biological activity.
• High quality product obtained under stringent conditions.
• For ex vivo research or bioproduction, additional documentation can be provided.

Please read our complete Animal-Free Statement

 

Publications for IL-6 (AFL206)(2)

Publications using AFL206

• Souheil-Antoine Younes IL-15 promotes activation and expansion of CD8+ T cells in HIV-1 infection J Clin Invest, 2016-06-20;126(7):2745-2756. 2016-06-20 [PMID: 27322062] (Bioassay, Human)
• Mack AA, Kroboth S, Rajesh D Generation of induced pluripotent stem cells from CD34+ cells across blood drawn from multiple donors with non-integrating episomal vectors. PLoS ONE, 2011-11-22;6(11):e27956. 2011-11-22 [PMID: 22132178] (Bioassay, Human)

FAQs for IL-6 (AFL206). (Showing 1 - 1 of 1 FAQs).

1. I was wondering if you know of any blocking antibodies against IL-6 or VEGF-C that can be used in cell culture to quench these cytokines?
   • We have no antibodies that are currently known to show blocking antibodies to VEGF-C. Please see this link to our IL-6 antibodies with blocking/neutralizing activity can be found at this link.

Bioinformatics

• Gene Symbol: IL6
• Uniprot:
  • P05231.1()