Recombinant Human IL-5 Protein

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1 μg/lane of Recombinant Human IL-5 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing bands at 13-15 kDa and 28-32 kDa, respectively.
Recombinant Human IL-5 (Catalog # 205-IL) stimulates cell proliferation of the TF-1 human erythroleukemic cell line. The ED50 for this effect is 0.04-0.2 ng/mL.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity

Order Details

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Catalog# & Formulation Size Price

Recombinant Human IL-5 Protein Summary

Details of Functionality
Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The ED50 for this effect is 0.04-0.2 ng/mL.
Source
Spodoptera frugiperda, Sf 21 (stably transfected)-derived human IL-5 protein
Ile20-Ser134
Accession #
N-terminal Sequence
Ile20
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
IL5
Purity
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
13 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
13-15 kDa, reducing conditions
Publications
Read Publications using
205-IL in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Purity
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 50 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human IL-5 Protein

  • BCDF mu
  • B-cell differentiation factor I
  • BCGFII
  • EDF
  • Eo-CSF
  • Eosinophil differentiation factor
  • IL5
  • IL-5
  • IL-5T-cell replacing factor
  • interleukin 5 (colony-stimulating factor, eosinophil)
  • interleukin-5
  • TRF
  • TRFB cell differentiation factor I

Background

Interleukin-5 (IL-5) is a secreted glycoprotein that belongs to the alpha -helical group of cytokines (1 ‑ 3). Unlike other family members, it is present as a covalently linked antiparallel dimer (4, 5). The cDNA for human IL-5 encodes a signal peptide and a 115 amino acid (aa) mature protein. Mature human IL-5 shares 70%, 70%, 62%, 71%, 70% and 66%, aa sequence identity with mouse, rat, canine, equine, feline and porcine IL-5, respectively and shows cross‑reactivity with mouse IL-5. IL-5 is primarily produced by CD4+ Th2 cells, but also by activated eosinophils, mast cells, EBV-transformed B cells, Reed-Sternberg cells in Hodgkin’s disease, and IL‑2‑stimulated invariant natural killer T cells (iNKT) (1 ‑ 3, 6 ‑ 8). IL-5 increases production and mobilization of eosinophils and CD34+ progenitors from the bone marrow and causes maturation of eosinophil precursors outside the bone marrow (1, 6, 9, 10). The receptor for human IL-5, mainly expressed by eosinophils, but also found on basophils and mast cells, consists of a unique ligand-binding subunit (IL-5 R alpha ) and a shared signal‑transducing subunit, beta c (3, 6, 11). IL-5 R alpha first binds IL-5 at low affinity, then associates with preformed beta c dimers, forming a high-affinity receptor (12). IL-5 also binds proteoglycans, potentially enhancing its activity (13). Soluble forms of IL-5 R alpha   antagonize IL-5 and can be found in vivo (10, 14). In humans, IL-5 primarily affects cells of the eosinophilic lineage, and promotes their differentiation, maturation, activation, migration and survival, while in mice IL-5 also enhances Ig class switching and release from B1 cells (1 ‑ 3, 9, 10, 15, 16). IL-5 also promotes differentiation of basophils and primes them for histamine and leukotriene release (17).

  1. Rosenberg, H. F. et al. (2007) J. Allergy Clin. Immunol. 119:1303.
  2. Elsas, P.X. and M. I. G. Elsas (2007) Curr. Med. Chem. 14:1925.
  3. Martinez-Moczygemba, M. and D. P. Huston (2003) J. Allergy Clin. Immunol. 112:653.
  4. Minamitake, Y. et al. (1990) J. Biochem. 107:292.
  5. McKenzie, A. N. et al. (1991) Mol. Immunol. 28:155.
  6. Shakoory, B. et al. (2004) J. Interferon Cytokine Res. 24:271.
  7. Lalani, T. et al. (1999) Ann. Allergy Asthma Immunol. 82:317.
  8. Sakuishi, K. et al. (2007) J. Immunol. 179:3452.
  9. Clutterbuck, E. J. et al. (1989) Blood 73:1504.
  10. Cameron, L. et al. (2000) J. Immunol. 164:1538.
  11. Tavernier, J. et al. (1991) Cell 66:1175.
  12. Zaks-Zilberman, M. et al. (2008) J. Biol. Chem. 283:13398.
  13. Lipscombe, R. et al. (1998) J. Leukocyte Biol. 63:342.
  14. Tavernier, J. et al. (2000) Blood 95:1600.
  15. Kopf, M. et al. (1996) Immunity 4:15.
  16. Horikawa, K. and K. Takatsu (2006) Immunology 118:497.
  17. Denburg, J. A. et al. (1991) Blood 77:1462.

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205-IL
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Publications for IL-5 (205-IL)(35)

We have publications tested in 3 confirmed species: Human, Guinea Pig, N/A.

We have publications tested in 3 applications: Bioassay, Cell Culture, ELISA (Standard).


Filter By Application
Bioassay
(31)
Cell Culture
(2)
ELISA (Standard)
(2)
All Applications
Filter By Species
Human
(32)
Guinea Pig
(1)
N/A
(2)
All Species
Showing Publications 1 - 10 of 35. Show All 35 Publications.
Publications using 205-IL Applications Species
Y Imoto, T Takabayash, M Sakashita, Y Kato, K Yoshida, M Kidoguchi, K Koyama, N Adachi, Y Kimura, K Ogi, Y Ito, M Kanno, M Okamoto, N Narita, S Fujieda Enhanced 15-Lipoxygenase 1 Production is Related to Periostin Expression and Eosinophil Recruitment in Eosinophilic Chronic Rhinosinusitis Biomolecules, 2020;10(11):. 2020 [PMID: 33218117] (Cell Culture, Human) Cell Culture Human
Y Cao, S Shin, DJ Carroll, JA O'Sullivan, BS Bochner Single-site, five-year experience with human eosinophil isolation by density gradient centrifugation and CD16 immunomagnetic negative separation BMC Res Notes, 2020;13(1):211. 2020 [PMID: 32276656] (Cell Culture, Human) Cell Culture Human
ME Coden, LF Loffredo, MT Walker, BM Jeong, K Nam, BS Bochner, H Abdala-Val, S Berdnikovs Fibrinogen Is a Specific Trigger for Cytolytic Eosinophil Degranulation J. Immunol., 2019;0(0):. 2019 [PMID: 31818982] (Bioassay, Human) Bioassay Human
X Qin, M Liu, S Zhang, C Wang, T Zhang The role of IL-36? and its regulation in eosinophilic inflammation in allergic rhinitis Cytokine, 2019;117(0):84-90. 2019 [PMID: 30831444] (Bioassay, Human) Bioassay Human
Karina T Barretto, Calvin M Swanson, Christopher L Nguyen, Douglas S Annis, Stephane J Esnault, Deane F Mosher, Mats W Johansson Control of cytokine-driven eosinophil migratory behavior by TGF-beta-induced protein (TGFBI) and periostin. PLoS ONE, 2018;0(0):1932-6203. 2018 [PMID: 30048528] (Bioassay, Human) Bioassay Human
V Bortnov, DS Annis, FJ Fogerty, KT Barretto, KB Turton, DF Mosher Myeloid-derived growth factor is a resident endoplasmic reticulum protein J. Biol. Chem., 2018;0(0):. 2018 [PMID: 29954947] (Bioassay, Human) Bioassay Human
F Bucher, J Lee, S Shin, MM Kim, YS Oh, S Ha, H Zhang, K Yea Interleukin-5 suppresses Vascular Endothelial Growth Factor-induced angiogenesis through STAT5 signaling Cytokine, 2018;0(0):. 2018 [PMID: 29921484] (Bioassay, Human) Bioassay Human
F Zellweger, P Buschor, G Hobi, D Brigger, CA Dahinden, PM Villiger, A Eggel IL-3 but not monomeric IgE regulates Fc?RI levels and cell survival in primary human basophils Cell Death Dis, 2018;9(5):510. 2018 [PMID: 29724998] (Bioassay, Human) Bioassay Human
JT Schroeder, AP Bieneman Activation of Human Basophils by A549 Lung Epithelial Cells Reveals a Novel IgE-Dependent Response Independent of Allergen J. Immunol., 2017;0(0):. 2017 [PMID: 28652400] (Bioassay, Human) Bioassay Human
MW Johansson, M Khanna, V Bortnov, DS Annis, CL Nguyen, DF Mosher IL-5-stimulated eosinophils adherent to periostin undergo stereotypic morphological changes and ADAM8-dependent migration Clin. Exp. Allergy, 2017;0(0):. 2017 [PMID: 28378503] (Bioassay, Human) Bioassay Human
Show All 35 Publications.

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Bioinformatics

Gene Symbol IL5
Uniprot