Recombinant Human IL-37/IL-1F7 Protein, CF

• Reactivity: Hu
• Applications: Binding Activity
• Format: Carrier-Free

Recombinant Human IL-37/IL-1F7 Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. When Recombinant Human (rh) IL‑18 R alpha /IL‑1 R5 Fc Chimera (Catalog # 816-LR) is immobilized at 2 μg/mL (100 μL/well), the concentration of rhIL-37/IL-1F7 Chimera that produces 50% optimal binding response is 20-120 ng/mL.
• Source: E. coli-derived human IL-37/IL-1F7 protein
  Lys27-Asp192, with an N-terminal Met
• Accession #: NP_775297
• N-terminal Sequence: Met
• Protein/Peptide Type: Recombinant Proteins
• Gene: IL37
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Binding Activity
• Theoretical MW: 18.6 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS and DTT.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human IL-37/IL-1F7 Protein, CF

• FIL1 zeta
• FIL1
• FIL1(ZETA)
• FIL1ZFIL1 zeta
• IL-1F7
• IL-1F7IL-1 zeta
• IL1H4IL1F7 (canonical product IL-1F7b)
• IL-1H4IL-1F7b (IL-1H4, IL-1H, IL-1RP1)
• IL-1RP1IL-1X protein
• IL1RP1interleukin 1 family member 7
• IL-1X
• IL37
• IL-37
• interleukin 1 family, member 7 (zeta)
• interleukin 1, zeta
• interleukin-1 family member 7
• Interleukin-1 homolog 4
• interleukin-1 superfamily z
• Interleukin-1 zeta
• Interleukin-1-related protein

Background

Human Interleukin-1 family member 7 (IL-1F7), also named FIL-1 zeta, IL‑37, IL‑1H4, IL-1HL and IL‑1RP1, is an anti-inflammatory member of the IL‑1 cytokine family (1-3). Alternative splicing generates multiple isoforms (IL‑37a through e) with deletions in the N-terminal region of the molecule (4-7). The longest IL-1F7 transcript encodes IL-37b, which shares approximately 21%, 24%, and 30% aa sequence identity with mature IL-1 alpha, IL‑1 beta, and IL-1ra, respectively. Mouse IL‑37 has not been reported, but human IL‑37b is active on mouse cells. Like IL‑1 alpha, IL‑1 beta and IL‑18, all of the IL‑37 variants lack a typical signal peptide. IL-37b is up-regulated by inflammatory stimuli in peripheral blood mononuclear cells (8). Experimental over-expression of IL‑37b in vivo limits the inflammatory response and protects mice from colitis and LPS-induced shock (8, 9). Both unprocessed and mature IL‑37b can form homodimers in solution (8, 10). Although IL‑37b will bind to IL‑18 R alpha with low affinity, this has no effect on IL‑18 receptor activity (7, 10, 11). Alternatively, IL‑37b will also bind to IL‑18 BP, generating a complex that interacts with IL‑18 R beta. This has the effect of attenuating IL‑18 activity via the IL‑18 receptor (11). IL‑37b can also function intracellularly. Following LPS‑induced cleavage of its propeptide, IL‑37b associates with Smad3 and translocates to the nucleus. This results in a reduction of pro‑inflammatory cytokine secretion (12).

1. Dinarello, C.A. and P. Bufler (2013) Sem. Immunol. 25:466.
2. Boraschi, D. et al. (2011) Eur. Cytokine Netw. 22:127.
3. Ding, V.A. et al. (2016) Med. Oncol. 33:68.
4. Smith, D.E. et al. (2000) J. Biol Chem 275:1169.
5. Kumar S. et al. (2000) J. Biol Chem 275:10308.
6. Busfield S.J. et al. (2000) Genomics 66:213.
7. Pan, G. et al. (2000) Cytokine 13:1.
8. Nold, M.F. et al. (2010) Nat. Immunol. 11:1014.
9. McNamee, E.N. et al. (2011) Proc. Natl. Acad. Sci. USA 108:16711.
10. Kumar S. et al. (2002) Cytokine 18:61.
11. Bufler, P. et al. (2002) Proc. Natl. Acad. Sci. USA 99:13723.
12. Sharma, S. et al. (2008) J. Immunol. 180:5477.

Publications for IL-37/IL-1F7 (1975-IL)(7)

Publications using 1975-IL

• N Ma, C Yuan, J Shi, Q Zhu, Y Liu, X Ma, B Li, W Gong, J Xue, G Lu, W Li, J Li Interleukin-37 protects against acinar cell pyroptosis in acute pancreatitis JCI Insight, 2022-11-08;0(0):. 2022-11-08 [PMID: 36166295] (In Vivo, Mouse)
• C Ye, W Zhang, K Hang, M Chen, W Hou, J Chen, X Chen, E Chen, L Tang, J Lu, Q Ding, G Jiang, B Hong, R He Extracellular IL-37 promotes osteogenic differentiation of human bone marrow mesenchymal stem cells via activation of the PI3K/AKT signaling pathway Cell Death Dis, 2019-10-03;10(10):753. 2019-10-03 [PMID: 31582734] (Bioassay, Human)
• Y Zhao, X Ni, P Xu, Q Liu, T Sun, X Liu, X Ji, J Qiu, J Li, S Wang, P Han, J Peng, M Hou, G Li Interleukin-37 reduces inflammation and impairs phagocytosis of platelets in immune thrombocytopenia (ITP) Cytokine, 2019-09-23;125(0):154853. 2019-09-23 [PMID: 31557634] (Bioassay, Human)
• L Li, J Wei, S Li, AM Jacko, NM Weathingto, RK Mallampall, J Zhao, Y Zhao The deubiquitinase USP13 stabilizes the anti-inflammatory receptor IL-1R8/Sigirr to suppress lung inflammation EBioMedicine, 2019-06-14;0(0):. 2019-06-14 [PMID: 31204278] (Bioassay, Human, Mouse)
• M Zhao, Y Li, C Guo, L Wang, H Chu, F Zhu, Y Li, X Wang, Q Wang, W Zhao, Y Shi, W Chen, L Zhang IL-37 isoform D downregulates pro-inflammatory cytokines expression in a Smad3-dependent manner Cell Death Dis, 2018-05-22;9(6):582. 2018-05-22 [PMID: 29789615] (Bioassay, Mouse)
• M Jiang, Y Wang, H Zhang, Y Ji, P Zhao, R Sun, C Zhang IL-37 inhibits invasion and metastasis in non-small cell lung cancer by suppressing the IL-6/STAT3 signaling pathway Thorac Cancer, 2018-03-25;0(0):. 2018-03-25 [PMID: 29575809] (Bioassay, Human)
• Wilson TR, Fridlyand J, Yan Y, Penuel E, Burton L, Chan E, Peng J, Lin E, Wang Y, Sosman J, Ribas A, Li J, Moffat J, Sutherlin DP, Koeppen H, Merchant M, Neve R, Settleman J Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors. Nature, 2012-07-26;487(7408):505-9. 2012-07-26 [PMID: 22763448] (Bioassay, Human)

Bioinformatics

• Gene Symbol: IL37
• Uniprot:
  • NP_775297(Human)