Recombinant Human IL-21 Protein, CF

Recombinant Human IL‑21 (Catalog # BT-021) has a molecular weight (MW) of 17.9 kDa as analyzed by SEC-MALS, suggesting that this protein is a monomer.

Recombinant Human IL-21 induces cell proliferation of B9 mouse hybridoma cells with an ED50 of 5.00-50.0 ng/mL.

2 μg/lane of Recombinant Human IL‑21 Protein (Catalog # BT-021) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 15-17 kDa, under reducing conditions.

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human IL-21 Protein, CF Summary

• Additional Information: Analyzed by SEC-MALS
• Details of Functionality: Measured in a cell proliferation assay using B9 mouse hybridoma cells. The ED₅₀ for this effect is 5.00-50.0 ng/mL.
• Source: E. coli-derived human IL-21 protein
  Gln32-Ser162 with a N-terminal Met
• Accession #: Q9HBE4.3
• N-terminal Sequence: Met
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE with quantitative densitometry by Coomassie® Blue Staining
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 15 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 15-17 kDa, under reducing conditions.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
• Purity: >95%, by SDS-PAGE with quantitative densitometry by Coomassie® Blue Staining
• Reconstitution Instructions: Reconstitute the 10 μg size at 100 μg/mL in PBS. Reconstitute all other sizes at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human IL-21 Protein, CF

• CVID11
• IL21
• IL-21
• IL-21Za11interleukin-21
• interleukin 21
• interleukin-21 isoform
• Za11

Background

IL-21 (Interleukin-21) is member of the family of cytokines that utilize the common gamma chain as a receptor subunit. This subunit is also a component of the receptors for IL-2, IL-4, IL-7, IL-9, and IL-15. IL-21 is produced by activated T follicular helper cells (Tfh), Th17 cells, and NKT cells. It exerts its biological effects through a heterodimeric receptor complex of the common gamma chain and the IL-21-specific IL-21 R. IL-21 supports Tfh cell development, immunoglobulin affinity maturation, plasma cell differentiation, B cell memory responses, and the migration of dendritic cells to draining lymph nodes. It costimulates the activation, proliferation, and survival of CD8+ T cells and NKT cells and promotes Th17 cell polarization. It blocks the generation of regulatory T cells and their suppressive effects on CD4+ T cells. In addition, IL-21 suppresses cutaneous hypersensitivity reactions by limiting allergen-specific IgE production and mast cell degranulation.

Bioinformatics

• Uniprot:
  • Q9HBE4.3()