Recombinant Human IL-17F (NS0-expressed) Protein

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity

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Recombinant Human IL-17F (NS0-expressed) Protein Summary

Details of Functionality
Measured by its ability to induce IL-6 secretion by NIH‑3T3 mouse embryonic fibroblast cells. The ED50 for this effect is 2-10 ng/mL.
Source
Mouse myeloma cell line, NS0-derived human IL-17F protein
Arg31-Gln163, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Arg21
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
IL17F
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
15.7 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
21 kDa, reducing conditions

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 25 μg/mL in sterile 4 mM HCl containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human IL-17F (NS0-expressed) Protein

  • Cytokine ML-1
  • IL17F
  • IL-17F
  • IL-17Finterleukin-17F
  • IL24
  • IL-24
  • interleukin 17F
  • Interleukin-24
  • ML-1
  • ML1interleukin-24

Background

Interleukin-17F (also known as ML-1) is a of the IL-17 family of cytokines. Members of this family are involved in tissue homeostasis and demonstrate a structural motif termed a cysteine-knot that characterizes a large superfamily of growth factors. Although most cysteine-knot superfamily members use three intrachain disulfide bonds to create a knot, IL-17 family molecules generate the same structural form with only two disulfide links (1 - 3). Human IL-17F is secreted as a glycosylated, 45 kDa, disulfide-linked homodimer. It is synthesized as a 153 amino acid (aa) precursor that contains a 20 aa signal sequence and a 133 aa mature region. As noted, the molecule contains a cysteine-knot that is formed from two sets of paired beta -strands that are connected by disulfide linkages (4 - 6). There is potentially one additional isoform that utilizes an alternate start site. This adds an additional 10 aa at the N-terminus (1, 7). 

When induced, IL-17F is preferentially translated as the 153 aa precursor (1). Initially, IL-17F was also reported as IL-24 (7). Since that time, the IL-24 designation has been reassigned to MDA-7, a member of the IL-10 family of molecules (note: IL-17E is synonymous with IL-25). Mature human IL-17F is 59%, 56%, 44% and 52% aa identical to mature rat, mouse, canine and bovine IL-17F, respectively. Within the IL-17 family, IL-17F is most closely related to IL-17/IL-17A at 50% aa identity (6). Human IL-17F is active on mouse and porcine cells (6). IL-17F is produced by activated CD4+ T cells, mast cells, basophils and monocytes (1, 4), and is inducible by IL-23 (4, 8). Targets for IL-17F include respiratory epithelium and endothelial cells which produce proinflammatory cytokines that promote neutrophil activation and influx (4, 5, 9, 10).

  1. Kawaguchi, M. et al. (2004) J. Allergy Clin. Immunol. 114:1265.
  2. Kolls, J.K. and A. Linden (2004) Immunity 21:467.
  3. Moseley, T.A. et al. (2003) Cytokine Growth Factor Rev. 14:155.
  4. Kawaguchi, M. et al. (2001) J. Immunol. 167:4430.
  5. Starnes, T. et al. (2001) J. Immunol. 167:4137.
  6. Hymowitz, S.G. et al. (2001) EMBO J. 20:5332.
  7. Hromes, R.A. and T.T. Starnes (2001) GenBank
    Accession # AAK83350.
  8. Langrish, C.L. et al. (2005) J. Exp. Med. 201:233.
  9. Mcallister, F. et al. (2005) J. Immunol. 175:404.
  10. Numasaki, M. et al. (2004) Immunol. Lett. 95:175.

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Bioinformatics

Gene Symbol IL17F
Entrez
Uniprot