Recombinant Human IL-17E Protein

Measured by its ability to induce CXCL1/GRO alpha secretion in HT‑29 human colon adenocarcinoma cells. The ED50 for this effect is 0.25-1.5 ng/mL.

• Reactivity: Hu
• Applications: Bioactivity

Recombinant Human IL-17E Protein Summary

• Details of Functionality: Measured by its ability to induce CXCL1/GRO alpha secretion in HT‑29 human colon adenocarcinoma cells. The ED₅₀ for this effect is 0.25-1.5 ng/mL.
• Source: E. coli-derived human IL-17E/IL-25 protein
  Tyr33-Gly177, with an N-terminal Met
• Accession #: Q9H293
• N-terminal Sequence: Met
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Gene: IL25
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 17 kDa (monomer).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile 4 mM HCl containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human IL-17E Protein

• IL17E
• IL-17E
• IL25
• IL-25
• interleukin 25
• Interleukin-17E
• interleukin-25

Background

The Interleukin‑17 (IL‑17) family of proteins are immunoregulatory cytokines that share a conserved cysteine‑rich region. IL‑17E, which is also known as IL‑25, promotes Th2‑biased immune responses. This is in contrast to other IL‑17 family members which promote Th1- and Th17‑biased inflammation. IL‑25 is an important mediator of allergic reactions and protection against intestinal parasites (1, 2). Mature human IL‑25 shares 80% amino acid sequence identity with mouse and rat IL‑25 (3, 4). During helminth infections and allergic reactions, IL‑25 is locally up‑regulated in intestinal and airway epithelial cells, atopic dermatitis skin lesions, and local Th2 cells, eosinophils, and basophils (4‑9). It binds to IL‑17 RB but also requires IL‑17 RA to exert its activity (3, 8, 10). IL‑25 acts on a variety of cell types which respond with increased production of Th2 cytokines (e.g. IL‑4, IL‑5, IL‑13) and reduced production of Th1 and Th17 cytokines (e.g. IFN‑ gamma , IL‑12, IL‑23, IL‑17A, IL‑17F) (4‑6, 8, 9, 11‑15). Airway IL‑25 can be activated by MMP‑7, a protease that is up‑regulated in airway epithelium in response to allergen exposure (16). Cleaved IL‑25 shows enhanced binding to IL‑17 RB and stronger induction of Th2 cytokines (16). The Th2 cytokines, in turn, trigger expansion of Th2 memory cells and anti‑inflammatory M2 macrophages, increased eosinophil mobilization and activation, and dendritic cell migration (4, 6, 9, 13). These actions promote protective anti‑helminth immune responses (4, 5) as well as allergic inflammation and airway hyperreactivity (11). The IL‑25 induced suppression of Th1 and Th17 cytokines limits Th17 cell expansion and disease pathology in autoimmunity and colitis (12, 15). IL‑25 also promotes vascular endothelial cell proliferation and assembly into tubular structures (7). It supports the integrity of the blood‑brain barrier and limits CD4⁺ T cell infiltration into the brain (17).

1. Saadoun, D. et al. (2011) Curr. Pharm. Des. 17:3781.
2. Iwakura, Y. et al. (2011) Immunity 34:149.
3. Lee, J. et al. (2001) J. Biol. Chem. 276:1660.
4. Fort, M.M. et al. (2001) Immunity 15:985.
5. Zhao, A. et al. (2010) J. Immunol. 185:6921.
6. Suzukawa, M. et al. (2012) J. Immunol. 189:3641.
7. Corrigan, C.J. et al. (2011) Proc. Natl. Acad. Sci. USA 108:1579.
8. Petersen, B.C. et al. (2012) Nat. Med. 18:751.
9. Wang, Y.-H. et al. (2007) J. Exp. Med. 204:1837.
10. Rickel, E.A. et al. (2008) J. Immunol. 181:4299.
11. Hurst, S.D. et al. (2002) J. Immunol. 169:443.
12. Kleinschek, M.A. et al. (2007) J. Exp. Med. 204:161.
13. Cao, Q. et al. (2011) J. Am. Soc. Nephrol. 22:1229.
14. Stock, P. et al. (2009) J. Immunol. 182:5116.
15. Caruso, R. et al. (2009) Gastroenterology 136:2270.
16. Goswami, S. et al. (2009) Nat. Immunol. 10:496.
17. Sonobe, Y. et al. (2009) J. Biol. Chem. 284:31834.

Publications for IL-17E/IL-25 (1258-IL)(21)

Publications using 1258-IL

• G Xiao, R Kumar, Y Komuro, J Burguet, V Kakarla, I Azizkhania, SA Sheth, CK Williams, XR Zhang, M Macknicki, A Brumm, R Kawaguchi, P Mai, N Kaneko, HV Vinters, ST Carmichael, LA Havton, C DeCarli, JD Hinman IL-17/CXCL5 signaling within the oligovascular niche mediates human and mouse white matter injury Cell Reports, 2022-12-20;41(12):111848. 2022-12-20 [PMID: 36543124] (Bioassay, Human)
• Rui Zheng, Yang Chen, Jianbo Shi, Kai Wang, Xuekun Huang, Yueqi Sun et al. Combinatorial IL-17RB, ST2, and TSLPR Signaling in Dendritic Cells of Patients With Allergic Rhinitis Frontiers in Cell and Developmental Biology 2020-04-03 [PMID: 32309281] (Bioassay, Human)
• T Petrova, J Pesic, K Pardali, M Gaestel, JSC Arthur p38 MAPK signalling regulates cytokine production in IL-33 stimulated Type 2 Innate Lymphoid cells Sci Rep, 2020-02-26;10(1):3479. 2020-02-26 [PMID: 32103032] (Cell Culture, Human)
• T Hochdörfer, C Winkler, K Pardali, J Mjösberg Expression of c-Kit discriminates between two functionally distinct subsets of human type 2 innate lymphoid cells Eur. J. Immunol., 2019-03-28;0(0):. 2019-03-28 [PMID: 30892687] (Bioassay, Human)
• X Qin, M Liu, S Zhang, C Wang, T Zhang The role of IL-36? and its regulation in eosinophilic inflammation in allergic rhinitis Cytokine, 2019-03-01;117(0):84-90. 2019-03-01 [PMID: 30831444] (Bioassay, Human)
• W Tang, SG Smith, W Du, A Gugilla, J Du, JP Oliveria, K Howie, BM Salter, GM Gauvreau, PM O'Byrne, R Sehmi Interleukin-25 and eosinophils progenitor cell mobilization in allergic asthma Clin Transl Allergy, 2018-02-13;8(0):5. 2018-02-13 [PMID: 29456832] (Bioassay, Human)
• V Lombardi, S Luce, H Moussu, L Morizur, C Gueguen, C Neukirch, S Chollet-Ma, L Mascarell, M Aubier, V Baron-Bodo, P Moingeon Effector and regulatory dendritic cells display distinct patterns of miRNA expression Immun Inflamm Dis, 2017-05-12;0(0):. 2017-05-12 [PMID: 28497578] (Bioassay, Human)
• Ling Y, Cypowyj S, Aytekin C, Galicchio M, Camcioglu Y, Nepesov S, Ikinciogullari A, Dogu F, Belkadi A, Levy R, Migaud M, Boisson B, Bolze A, Itan Y, Goudin N, Cottineau J, Picard C, Abel L, Bustamante J, Casanova J, Puel A Inherited IL-17RC deficiency in patients with chronic mucocutaneous candidiasis. J Exp Med, 2015-04-27;212(5):619-31. 2015-04-27 [PMID: 25918342] (Bioassay, Human)
• Yao , Xiujuan, Wang , Wei, Li , Yan, Huang , Ping, Zhang , Qian, Wang , Jingjing, Wang , Wen, Lv , Zhe, An , Yunqing, Qin , Jianguo, Corrigan , Chris J, Huang , Kewu, Sun , Yongchan, Ying , Sun IL-25 induces airways angiogenesis and expression of multiple angiogenic factors in a murine asthma model. Respir Res, 2015-03-18;16(0):39. 2015-03-18 [PMID: 25889697] (Bioassay, Human)
• Wu L, Wang C, Boisson B, Misra S, Rayman P, Finke J, Puel A, Casanova J, Li X The differential regulation of human ACT1 isoforms by Hsp90 in IL-17 signaling. J Immunol, 2014-07-14;193(4):1590-9. 2014-07-14 [PMID: 25024377] (Bioassay, Human)
• Teunissen M, Munneke J, Bernink J, Spuls P, Res P, Te Velde A, Cheuk S, Brouwer M, Menting S, Eidsmo L, Spits H, Hazenberg M, Mjosberg J Composition of innate lymphoid cell subsets in the human skin: enrichment of NCR(+) ILC3 in lesional skin and blood of psoriasis patients. J Invest Dermatol, 2014-03-21;134(9):2351-60. 2014-03-21 [PMID: 24658504] (Bioassay, Human)
• Biancheri P, Di Sabatino A, Ammoscato F, Facciotti F, Caprioli F, Curciarello R, Hoque S, Ghanbari A, Joe-Njoku I, Giuffrida P, Rovedatti L, Geginat J, Corazza G, MacDonald T Absence of a role for interleukin-13 in inflammatory bowel disease. Eur J Immunol, 2014-02-01;44(2):370-85. 2014-02-01 [PMID: 24338958] (Bioassay, Human)
• Boisson B, Wang C, Pedergnana V, Wu L, Cypowyj S, Rybojad M, Belkadi A, Picard C, Abel L, Fieschi C, Puel A, Li X, Casanova J An ACT1 mutation selectively abolishes interleukin-17 responses in humans with chronic mucocutaneous candidiasis. Immunity, 2013-10-10;39(4):676-86. 2013-10-10 [PMID: 24120361] (Bioassay, Human)
• Hvid M, Vestergaard C, Kemp K IL-25 in atopic dermatitis: a possible link between inflammation and skin barrier dysfunction? J. Invest. Dermatol., 2010-09-23;131(1):150-7. 2010-09-23 [PMID: 20861853] (Bioassay, Human)
• Terrier B, Bieche I, Maisonobe T, Laurendeau I, Rosenzwajg M, Kahn JE, Diemert MC, Musset L, Vidaud M, Sene D, Costedoat-Chalumeau N, Le Thi-Huong D, Amoura Z, Klatzmann D, Cacoub P, Saadoun D Interleukin-25: a cytokine linking eosinophils and adaptive immunity in Churg-Strauss syndrome. Blood, 2010-08-20;116(22):4523-31. 2010-08-20 [PMID: 20729468] (Bioassay, Human)
• Xu G, Zhang L, Wang DY, Xu R, Liu Z, Han DM, Wang XD, Zuo KJ, Li HB Opposing roles of IL-17A and IL-25 in the regulation of TSLP production in human nasal epithelial cells. Allergy, 2009-11-25;65(5):581-9. 2009-11-25 [PMID: 19968632] (Bioassay, Human)
• Duluc D, Corvaisier M, Blanchard S, Catala L, Descamps P, Gamelin E, Ponsoda S, Delneste Y, Hebbar M, Jeannin P Interferon-gamma reverses the immunosuppressive and protumoral properties and prevents the generation of human tumor-associated macrophages. Int. J. Cancer, 2009-07-15;125(2):367-73. 2009-07-15 [PMID: 19378341] (Bioassay, Human)
• Rickel EA, Siegel LA, Yoon BR, Rottman JB, Kugler DG, Swart DA, Anders PM, Tocker JE, Comeau MR, Budelsky AL Identification of functional roles for both IL-17RB and IL-17RA in mediating IL-25-induced activities. J. Immunol., 2008-09-15;181(6):4299-310. 2008-09-15 [PMID: 18768888] (Bioassay, Human)
• Cherry WB, Yoon J, Bartemes KR, Iijima K, Kita H A novel IL-1 family cytokine, IL-33, potently activates human eosinophils. J. Allergy Clin. Immunol., 2008-06-01;121(6):1484-90. 2008-06-01 [PMID: 18539196] (Bioassay, Human)
• Létuvé S, Lajoie-Kadoch S, Audusseau S, Rothenberg ME, Fiset PO, Ludwig MS, Hamid Q IL-17E upregulates the expression of proinflammatory cytokines in lung fibroblasts. J. Allergy Clin. Immunol., 2006-02-08;117(3):590-6. 2006-02-08 [PMID: 16522458] (Bioassay, Human)
• Wong CK, Cheung PF, Ip WK, Lam CW Interleukin-25-induced chemokines and interleukin-6 release from eosinophils is mediated by p38 mitogen-activated protein kinase, c-Jun N-terminal kinase, and nuclear factor-kappaB. Am. J. Respir. Cell Mol. Biol., 2005-04-28;33(2):186-94. 2005-04-28 [PMID: 15860795] (Bioassay, Human)

Bioinformatics

• Gene Symbol: IL25
• Uniprot:
  • Q9H293()