Recombinant Human IL-17E (Human Cell-expressed) Protein

Recombinant Human IL-17E/IL-25 (Catalog # 8134-IL) induces CXCL1/GRO alpha secretion in HT‑29 human colon adenocarcinoma cells. The ED50 for this effect is 0.25-1.5 ng/mL.

1 μg/lane ofRecombinant Human IL-17E (Catalog # 8134-IL) was resolved with SDS-PAGE underreducing (R) and non-reducing (NR) conditions and visualized by silverstaining, showing R bands at 20-28 kDa and NR bands at 37-43 kDa.

• Reactivity: Hu
• Applications: Bioactivity

Recombinant Human IL-17E (Human Cell-expressed) Protein Summary

• Details of Functionality: Measured by its ability to induce CXCL1/GRO alpha secretion in HT‑29 human colon adenocarcinoma cells. The ED₅₀ for this effect is 0.25-1.5 ng/mL.
• Source: Human embryonic kidney cell, HEK293-derived human IL-17E/IL-25 protein
  Tyr33-Gly177
• Accession #: Q9H293
• N-terminal Sequence: Tyr33
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Gene: IL25
• Purity: >95%, by SDS-PAGE with silver staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 17 kDa (monomer).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 20-28 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in HCl with BSA as a carrier protein.
• Purity: >95%, by SDS-PAGE with silver staining.
• Reconstitution Instructions: Reconstitute at 100 μg/mL in 4 mM HCl containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human IL-17E (Human Cell-expressed) Protein

• IL17E
• IL-17E
• IL25
• IL-25
• interleukin 25
• Interleukin-17E
• interleukin-25

Background

The Interleukin-17 (IL-17) family of proteins are immunoregulatory cytokines that share a conserved cysteine-rich region. IL-17E, which is also known as IL-25, promotes Th2-biased immune responses. This is in contrast to other IL-17 family members which promote Th1- and Th17-biased inflammation. IL-25 is an important mediator of allergic reactions and protection against intestinal parasites (1, 2). Mature human IL-25 shares 80% amino acid sequence identity with mouse and rat IL-25 (3, 4). During helminth infections and allergic reactions, IL-25 is locally up-regulated in intestinal and airway epithelial cells, atopic dermatitis skin lesions, and local Th2 cells, eosinophils, and basophils (4-9). It binds to IL-17 RB but also requires IL-17 RA to exert its activity (3, 8, 10). IL-25 acts on a variety of cell types which respond with increased production of Th2 cytokines (e.g. IL-4, IL-5, IL-13) and reduced production of Th1 and Th17 cytokines (e.g. IFN-gamma , IL-12, IL-23, IL-17A, IL-17F) (4-6, 8, 9, 11-15). Airway IL-25 can be activated by MMP-7, a protease that is up-regulated in airway epithelium in response to allergen exposure (16). Cleaved IL-25 shows enhanced binding to IL-17 RB and stronger induction of Th2 cytokines (16). The Th2 cytokines, in turn, trigger expansion of Th2 memory cells and
anti‑inflammatory M2 macrophages, increased eosinophil mobilization and activation, and dendritic cell migration (4, 6, 9, 13). These actions promote protective
anti‑helminth immune responses (4, 5) as well as allergic inflammation and airway hyperreactivity (11). The IL-25 induced suppression of Th1 and Th17 cytokines limits Th17 cell expansion and disease pathology in autoimmunity and colitis [12, 15]. IL-25 also promotes vascular endothelial cell proliferation and assembly into tubular structures (7). It supports the integrity of the blood-brain barrier and limits CD4⁺ T cell infiltration into the brain (17).

1. Saadoun, D. et al. (2011) Curr. Pharm. Des. 17:3781.
2. Iwakura, Y. et al. (2011) Immunity 34:149.
3. Lee, J. et al. (2001) J. Biol. Chem. 276:1660.
4. Fort, M.M. et al. (2001) Immunity 15:985.
5. Zhao, A. et al. (2010) J. Immunol. 185:6921.
6. Suzukawa, M. et al. (2012) J. Immunol. 189:3641.
7. Corrigan, C.J. et al. (2011) Proc. Natl. Acad. Sci. USA 108:1579.
8. Petersen, B.C. et al. (2012) Nat. Med. 18:751.
9. Wang, Y.-H. et al. (2007) J. Exp. Med. 204:1837.
10. Rickel, E.A. et al. (2008) J. Immunol. 181:4299.
11. Hurst, S.D. et al. (2002) J. Immunol. 169:443.
12. Kleinschek, M.A. et al. (2007) J. Exp. Med. 204:161.
13. Cao, Q. et al. (2011) J. Am. Soc. Nephrol. 22:1229.
14. Stock, P. et al. (2009) J. Immunol. 182:5116.
15. Caruso, R. et al. (2009) Gastroenterology 136:2270.
16. Goswami, S. et al. (2009) Nat. Immunol. 10:496.
17. Sonobe, Y. et al. (2009) J. Biol. Chem. 284:31834.

Publications for IL-17E/IL-25 (8134-IL)(3)

Publications using 8134-IL

• Ben, J;Moududee, S;Ge, D;Zhou, P;Wang, A;Liu, Y;You, Z; SCFFBXW11 Complex Targets Interleukin-17 Receptor A for Ubiquitin-Proteasome-Mediated Degradation Biomedicines 2024-03-28 [PMID: 38672111] (Bioassay, Human)
• Q Miao, Y Wang, YG Liu, YX Ren, H Guan, Z Li, W Xu, L Xiang Seasonal variation in circulating group 2 innate lymphoid cells in mugwort-allergic asthmatics during and outside pollen season Allergy Asthma Clin Immunol, 2018-02-09;14(0):6. 2018-02-09 [PMID: 29449864] (Bioassay, Human)
• Yuan Zhang, Ying Wang, Ming-Qing Li, Jie Duan, Deng-Xuan Fan, Li-Ping Jin IL-25 promotes Th2 bias by upregulating IL-4 and IL-10 expression of decidual ??T cells in early pregnancy. Experimental and Therapeutic Medicine, 2017-12-15;0(0):1792-0981. 2017-12-15 [PMID: 29434775] (Bioassay, Human)

Bioinformatics

• Gene Symbol: IL25
• Uniprot:
  • Q9H293()