Recombinant Human IL-17D Protein, CF

• Reactivity: Hu
• Applications: Binding Activity
• Format: Carrier-Free

Recombinant Human IL-17D Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. When Recombinant Human IL‑17D is present at 1 μg/mL, the concentration of Recombinant Human IL‑17B R Fc Chimera (Catalog # 1207‑BR) that produces 50% of the optimal binding response is approximately 0.4‑2 μg/mL.
• Source: E. coli-derived human IL-17D protein
  Ala18-Pro202
• Accession #: Q8TAD2.1
• N-terminal Sequence: Ala18
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Gene: IL17D
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Endotoxin Note: <1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Binding Activity
• Theoretical MW: 20 kDa (monomer).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile 4 mM HCl.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human IL-17D Protein, CF

• FLJ30846
• IL17D
• IL-17D
• IL-17Dinterleukin 27
• IL-22
• IL-27interleukin-17D
• IL27interleukin-27
• interleukin 17D
• Interleukin-27

Background

The Interleukin-17 (IL-17) family proteins, comprising six members (IL-17, IL-17B through IL-17F), are secreted, structurally related proteins that share a conserved cysteine-knot fold near the C-terminus, but have considerable sequence divergence at the N-terminus (1, 2). With the exception of IL-17B, which exists as a
non‑covalently linked dimer, all IL-17 family members are disulfide-linked dimers (3). IL‑17 family proteins are pro-inflammatory cytokines that induce local cytokine production and are involved in the regulation of immune functions (1, 2). Two receptors (IL-17 R, and IL-17B R), which are activated by IL-17 family members, have been identified. In addition, at least three additional orphan type I transmembrane receptors with homology to IL-17 R, including IL-17 RL (IL-17 RC), IL-17 RD, and
IL‑17 RE, have also been reported (1-4). The functions of IL-17 RC, D, and E are not known.

Human IL-17D cDNA encodes a 202 amino acid (aa) residues protein with a putative 17 aa signal peptide (5). Human and mouse IL-17D share 78% sequence identity. Among IL-17 family members, IL-17D is most closely related to IL-17B, sharing 27% aa sequence homology (5, 6). IL-17D is expressed preferentially in skeletal muscle, heart, adipose tissue, lung, pancreas, and nervous system (1, 5). Like other IL-17 family members, IL-17D modulates immune responses indirectly by stimulating the production of myeloid growth factors and chemokines including IL-6, IL-8, and GM-CSF (5). IL-17D has also been shown to suppress the proliferation of myeloid progenitors in colony formation assays. The receptor of IL-17D has not yet been identified. However, stimulation of IL-8 production by IL-17D is mediated through the activation of nuclear factor kappa-B (5). The IL-17D preparations from R&D Systems have been found to bind immobilized recombinant IL17B R/Fc in a functional ELISA.

1. Aggarwal, S. and A.L. Gurney (2002) J. Leukoc. Biol. 71:1.
2. Moseley, T.A. et al. (2003) Cytokine & Growth Factor Rev. 14:155.
3. Hymowitz, S.G. et al. (2001) EMBO J. 20:5332.
4. Haudenschild, D. et al. (2002) J. Biol. Chem. 277:4309.
5. Starnes, T. et al. (2002) J. Immunol. 169:642.
6. Li, H. et al. (2000) Proc. Natl. Acad. Sci. USA 97:773.

Publications for IL-17D (1504-IL/CF)(1)

Publication using 1504-IL/CF

• G Xiao, R Kumar, Y Komuro, J Burguet, V Kakarla, I Azizkhania, SA Sheth, CK Williams, XR Zhang, M Macknicki, A Brumm, R Kawaguchi, P Mai, N Kaneko, HV Vinters, ST Carmichael, LA Havton, C DeCarli, JD Hinman IL-17/CXCL5 signaling within the oligovascular niche mediates human and mouse white matter injury Cell Reports, 2022-12-20;41(12):111848. 2022-12-20 [PMID: 36543124] (Bioassay, Human)

Bioinformatics

• Gene Symbol: IL17D
• Uniprot:
  • Q8TAD2.1()