Recombinant Human IL-12 Protein


1 µg/lane of Recombinant Human IL-12 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining. Single bands were observed at 41 kDa (p40) and 29 kDa (p35), under more
Recombinant Human IL-12 (Catalog #219‑IL) stimulates proliferation inPHA-activated human T lymphoblasts. The ED50 for this effect is 0.01-0.05 ng/mL.

Product Details

Reactivity HuSpecies Glossary
Applications Bioactivity

Order Details

View Available Formulations
Catalog# & Formulation Size Price

Recombinant Human IL-12 Protein Summary

Additional Information
HEK293-expressed Version Available!
Details of Functionality
Measured in a cell proliferation assay using PHA-stimulated human T lymphoblasts. Symons, J.A. et al. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 272. The ED50 for this effect is 0.01-0.05 ng/mL.
The specific activity of Recombinant Human IL-12 is approximately 1.1 x 104 units/μg, which is calibrated against recombinant human IL-12 WHO Standard (NIBSC code: 95/544).
Spodoptera frugiperda, Sf 21 (baculovirus)-derived human IL-12 protein
Human IL-12 p40
Accession # P29460
Human IL-12 p35
Accession # P29459
N-terminus C-terminus
Accession #
N-terminal Sequence
Ile23 (p40) & Arg23 (p35)
Structure / Form
Disulfide-linked heterodimer
Protein/Peptide Type
Recombinant Proteins
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.


Theoretical MW
34.7 kDa (p40) & 22.5 kDa (p35).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
41 kDa (p40) & 29 kDa (p35), reducing conditions
Read Publications using
219-IL in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 50 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human IL-12 Protein

  • CLMF p35
  • CLMF1
  • Cytotoxic lymphocyte maturation factor 35 kDa subunit
  • IL-12 subunit p35
  • IL12
  • IL-12
  • IL-12, subunit p35
  • IL12A
  • interleukin 12, p35
  • interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocytematuration factor 1, p35)
  • interleukin-12 alpha chain
  • interleukin-12 subunit alpha
  • natural killer cell stimulatory factor 1, 35 kD subunit
  • NF cell stimulatory factor chain 1
  • NK cell stimulatory factor chain 1
  • NKSF1
  • p35


Interleukin 12, also known as natural killer cell stimulatory factor (NKSF) or cytotoxic lymphocyte maturation factor (CLMF), is a pleiotropic cytokine originally identified in the medium of activated human B lymphoblastoid cell lines. The p40 subunit of IL-12 has been shown to have extensive amino acid sequence homology to the extracellular domain of the human IL-6 receptor while the p35 subunit shows distant but significant sequence similarity to IL-6, G-CSF, and chicken MGF. These observations have led to the suggestion that IL-12 might have evolved from a cytokine/soluble receptor complex. Human and murine IL-12 share 70% and 60% amino acid sequence homology in their p40 and p35 subunits, respectively. IL-12 apparently shows species specificity with human IL-12 reportedly showing minimal activity in the murine system.

IL-12 is produced by macrophages and B lymphocytes and has been shown to have multiple effects on T cells and natural killer (NK) cells. These effects include inducing production of IFN-gamma and TNF by resting and activated T and NK cells, synergizing with other IFN-gamma  inducers at both the transcriptional and post-transcriptional levels. This interaction induces IFN-gamma  gene expression, enhancing the cytotoxic activity of resting NK and T cells, inducing and synergizing with IL-2 in the generation of lymphokine-activated killer (LAK) cells, acting as a co-mitogen to stimulate proliferation of resting T cells, and inducing proliferation of activated T and NK cells. Current evidence indicates that IL‑12, produced by macrophages in response to infectious agents, is a central mediator of the cell‑mediated immune response by its actions on the development, proliferation, and activities of TH1 cells. In its role as the initiator of cell-mediated immunity, it has been suggested that IL-12 has therapeutic potential as a stimulator of cell-mediated immune responses to microbial pathogens, metastatic cancers, and viral infections such as AIDS.

Customers Who Viewed This Item Also Viewed...

Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu, Mu, Rt
Applications: WB, Simple Western, IHC, ICC, KO
Species: Hu
Applications: WB, IHC, ICC
Species: Mu
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P, IP, KD
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P, IP
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu
Applications: IHC
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu, Mu
Applications: WB, IHC, IHC-P
Species: Mu, Rt
Applications: WB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P
Species: Hu, Mu, Rt, Po, Ch, ChHa, Ma
Applications: WB, Simple Western, EM, Flow, HEStain, IB, ICC/IF, IHC, IHC-Fr, IHC-P, CyTOF-ready, IHC-FrFl, KD, KO

Publications for IL-12 (219-IL)(113)

We have publications tested in 4 confirmed species: Human, Mouse, N/A, Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque).

We have publications tested in 6 applications: Bioassay, CAR-T (Bioassay), Cell Culture, ELISA (Control), ELISA (Specificity Testing), ELISA (Standard).

Filter By Application
CAR-T (Bioassay)
Cell Culture
ELISA (Control)
ELISA (Specificity Testing)
ELISA (Standard)
All Applications
Filter By Species
Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
All Species
Showing Publications 1 - 10 of 113. Show All 113 Publications.
Publications using 219-IL Applications Species
UI Wu, KN Olivier, DB Kuhns, DL Fink, EP Sampaio, AM Zelazny, SJ Shallom, BE Marciano, MS Lionakis, SM Holland Patients with Idiopathic Pulmonary Nontuberculous Mycobacterial Disease Have Normal Th1/Th2 Cytokine Responses but Diminished Th17 Cytokine and Enhanced Granulocyte-Macrophage Colony-Stimulating Factor Production Open Forum Infect Dis, 2019;6(12):ofz484. 2019 [PMID: 31807607] (Bioassay, Human) Bioassay Human
R Khoshnevis, N Nekooei-Ma, C Klein, D Kotlarz, M Behnam, V Ostadi, M Yaran, A Rezaei, R Sherkat IL-12Rbeta1 deficiency corresponding to concurrency of two diseases, mendelian susceptibility to mycobacterial disease and Crohn&#039;s disease J Clin Tuberc Other Mycobact Dis, 2019;17(0):100123. 2019 [PMID: 31788565] (Bioassay, Human) Bioassay Human
AC Pecher, F Kettemann, E Asteriti, H Schmid, S Duerr-Stoe, H Keppeler, JC Henes, R Klein, C Hinterleit, KA Secker, C Schneidawi, L Kanz, D Schneidawi Invariant natural killer T cells are functionally impaired in patients with systemic sclerosis Arthritis Res. Ther., 2019;21(1):212. 2019 [PMID: 31615552] (Bioassay, Human) Bioassay Human
T Hofland, I de Weerdt, H Ter Burg, R de Boer, S Tannheimer, SH Tonino, AP Kater, E Eldering Dissection of the Effects of JAK and BTK Inhibitors on the Functionality of Healthy and Malignant Lymphocytes J. Immunol., 2019;0(0):. 2019 [PMID: 31511358] (Bioassay, Human) Bioassay Human
H Wang, L Shen, X Sun, F Liu, W Feng, C Jiang, X Chu, X Ye, C Jiang, Y Wang, P Zhang, M Zang, D Zhu, Y Bi Adipose group 1 innate lymphoid cells promote adipose tissue fibrosis and diabetes in obesity Nat Commun, 2019;10(1):3254. 2019 [PMID: 31332184] (Cell Culture, Human) Cell Culture Human
JH Bernink, Y Ohne, MBM Teunissen, J Wang, J Wu, L Krabbendam, C Guntermann, R Volckmann, J Koster, S van Tol, I Ramirez, Y Shrestha, MA de Rie, H Spits, X Romero Ros, AA Humbles c-Kit-positive ILC2s exhibit an ILC3-like signature that may contribute to IL-17-mediated pathologies Nat. Immunol., 2019;0(0):. 2019 [PMID: 31263279] (Cell Culture, Human) Cell Culture Human
J Kristoff, ML Palma, TM Garcia-Bat, C Shen, N Sluis-Crem, P Gupta, CR Rinaldo, RB Mailliard Type 1-programmed dendritic cells drive antigen-specific latency reversal and immune elimination of persistent HIV-1 EBioMedicine, 2019;0(0):. 2019 [PMID: 30952614] (Bioassay, Human) Bioassay Human
T Hochdörfer, C Winkler, K Pardali, J Mjösberg Expression of c-Kit discriminates between two functionally distinct subsets of human type 2 innate lymphoid cells Eur. J. Immunol., 2019;0(0):. 2019 [PMID: 30892687] (Bioassay, Human) Bioassay Human
LD Uppendahl, M Felices, L Bendzick, C Ryan, B Kodal, P Hinderlie, KLM Boylan, APN Skubitz, JS Miller, MA Geller Cytokine-induced memory-like natural killer cells have enhanced function, proliferation, and in vivo expansion against ovarian cancer cells Gynecol. Oncol., 2019;0(0):. 2019 [PMID: 30658847] (Bioassay, Human) Bioassay Human
P Gruarin, S Maglie, M De Simone, B Häringer, C Vasco, V Ranzani, R Bosotti, JS Noddings, P Larghi, F Facciotti, ML Sarnicola, M Martinovic, MC Crosti, M Moro, RL Rossi, ME Bernardo, F Caprioli, F Locatelli, G Rossetti, S Abrignani, M Pagani, J Geginat Eomesodermin controls a unique differentiation program in human IL-10 and IFN-? co-producing regulatory T-cells Eur. J. Immunol., 2018;0(0):. 2018 [PMID: 30431161] (Bioassay, Human) Bioassay Human
Show All 113 Publications.

Reviews for IL-12 (219-IL) (0)

There are no reviews for IL-12 (219-IL). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

FAQs for IL-12 (219-IL) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Other Available Formats

Additional IL-12 Products

Bioinformatics Tool for IL-12 (219-IL)

Discover related pathways, diseases and genes to IL-12 (219-IL). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for IL-12 (219-IL)

Discover more about diseases related to IL-12 (219-IL).

Pathways for IL-12 (219-IL)

View related products by pathway.

PTMs for IL-12 (219-IL)

Learn more about PTMs related to IL-12 (219-IL).

Blogs on IL-12.

Success of combined IL-10 and IL-12 therapy in colon cancer depends on IFN-gamma and gut barrier integrity
By Jamshed Arslan, Pharm. D., PhD. Colon cancer is responsible for over 600,000 deaths per year worldwide. Colon cancer can be classified into two categories: mismatch repair (MMR)-deficient and MMR-proficient cancers...  Read full blog post.

You complete me: Natural killer cells need TGF-beta inhibition to effectively combat cancers
By Jamshed Arslan Pharm.D. Natural killer (NK) cells are lymphocytes of the innate immune system that were first discovered for their “natural” ability to kill cancer cells. To use NK cells as anti-cancer therapy, t...  Read full blog post.

Ly6G - a marker for monocytes, granulocytes and neutrophils
Ly6G (Lymphocyte antigen 6 complex locus G6D) is a 21-25kD glycosylphosphatidylinositol (GPI)-linked differentiation antigen that is expressed by myeloid-derived cells in a tightly developmentally-regulated manner in the bone marrow. Monocytes expres...  Read full blog post.

Customers Who Bought This Also Bought

Contact Information

Product PDFs


Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.


Review this Product

Be the first to review our Recombinant Human IL-12 Protein and receive a gift card or discount.


Gene Symbol IL12A