Recombinant Human ICOS Fc Chimera (Catalog # 169-CS) inhibits rhB7-H2/FcChimera (Catalog # 165-B7) induced human T cell proliferation in the presence of anti-CD3. The ED50 for this effect is 1‑4 µg/mL.
Recombinant Human ICOS Fc Chimera Protein, CF Summary
Details of Functionality
Measured by its ability to inhibit human T cell proliferation induced by rhB7-H2/Fc Chimera in the presence of anti-CD3. Wang, S. et al. (2000) Blood 96:2808. The ED50 for this effect is 1-4 µg/mL in the presence of 3 µg/mL Recombinant Human B7‑H2 Fc Chimera (Catalog # 165-B7) and at least 20 ng/mL of anti-CD3. Optimal dilutions should be determined by each laboratory for each application.
Mouse myeloma cell line, NS0-derived human ICOS protein
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
<0.10 EU per 1 μg of the protein by the LAL method.
40.3 kDa (monomer). Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Inducible co-stimulator (ICOS), also called AILIM (activiation-inducible lymphocyte immunomediatory molecule) and CRP-1 (CD28-related protein-1), is a member of the growing CD28 family of immune costimulatory receptors. Other family members are CD28, CTLA-4 and PD-1. Human ICOS is a homodimeric type I transmembrane protein consisting of 199 amino acids (aa) with a putative 20 aa signal sequence, a 121 aa extracellular domain, a 23 aa transmembrane region, and a 35 aa cytoplasmic domain. ICOS shares approximately 39% amino acid similarity with CD28 and CTLA-4. Human and mouse ICOS share approximately 72% amino acid identity. ICOS is expressed on most CD45RO+ cells. ICOS expression is up-regulated within approximately 24 - 48 hours of activation on Th primed cells. B7-H2, a member of the B7 family of co-stimulatory ligands, has been identified as the ICOS ligand. The B7-H2/ICOS interaction appears to play roles in T cell dependent B cell activation and Th differentiation.
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Coyle, A.J. et al. (2000) Immunity 13:95.
Coyle, A.J. and J.C. Gutierrez-Ramos (2001) Nat. Immunol. 2:203.
Gonzalo, J.A. et al. (2001) J. Immunol. 166:1.
Hutloff, A. et al. (1999) Nature 397:263.
Mages, H.W. et al. (2000) Eur. J. Immunol. 30:1040.
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