Recombinant Human HMGB1 Protein, CF

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human HMGB1 Protein, CF Summary

• Details of Functionality: Measured by its ability to induce TNF-alpha secretion by RAW 264.7 mouse monocyte/macrophage cells. The ED₅₀ for this effect is 2.5-12.5 μg/mL.
• Source: Mouse myeloma cell line, NS0-derived human HMGB1/HMG-1 protein
  Met1-Glu215
• Accession #: P09429
• N-terminal Sequence: Met1 & Gly2
• Protein/Peptide Type: Recombinant Proteins
• Gene: HMGB1
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 25 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 28-34 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining
• Reconstitution Instructions: Reconstitute at 200 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human HMGB1 Protein, CF

• Amphoterin
• high mobility group box 1
• High mobility group protein 1
• high mobility group protein B1
• high-mobility group (nonhistone chromosomal) protein 1
• high-mobility group box 1
• HMG1
• HMG-1
• HMG1DKFZp686A04236
• HMG3
• HMGB1
• SBP-1
• Sulfoglucuronyl carbohydrate binding protein

Background

High-mobility Group Box 1 protein (HMGB1), also known as HMG1 or Amphoterin, is a member of the high mobility group box family of non-histone chromosomal proteins (1-3). Human HMGB1 is expressed as a 25 kDa single chain protein containing two globular positively charged DNA-binding domains (HMG boxes A and B) and a negatively charged C-terminal region that contains only Asp and Glu residues (4, 5). Posttranslational modification of HMGB1, including acetylation, phosphorylation, and methylation, affects HMGB1 localization, receptor interactions, and bioactivity (3). An intramolecular disulfide bond between Cys23 and Cys45 as well as the presence of the unpaired Cys106 thiol are critical for HMGB1-induced pro-inflammatory TNF-alpha secretion (2, 6). Alternatively, fully reduced HMGB1 acts as a potent chemoattractant for neutrophils and monocytes (7). HMGB1 can be localized to the nucleus or cytoplasm and can also be secreted despite its lack of a signal peptide (2). HMGB1 binds DNA in a non-sequence specific manner and may act as a structural cofactor during gene transcription (8). Acetylation of HMGB1 results in its cytoplasmic localization and eventual secretion (9). HMGB1 can be secreted by multiple cell types, and it is also released upon cell necrosis, apoptosis, and pyroptosis (2, 3). HMGB1 is widely recognized as a multifunctional alarmin that stimulates inflammation upon sterile or infectious insult. Receptors for HMGB1 include TLR2, TLR4, TLR9, Syndecan-3, Siglec-10, Integrin alpha M beta 2, CXCR4, TIM-3, and RAGE (1, 2). It is implicated in the pathogenesis of a broad range of diseases including atherosclerosis, sepsis, cancer, neurodegenerative diseases, and autoimmunity (3, 10-13).

1. Yanai, H. et al. (2012) Trends Immunol. 33:633.
2. Yang, H. et al. (2013) J. Leukoc. Biol. 93:865.
3. Harris, H.E. et al. (2012) Nat. Rev. Rheumatol. 8:195.
4. Degryse, B. and M. de Virgilio (2003) FEBS Lett. 553:11.
5. Wen, L. et al. (1989) Nucleic Acids Res. 17:1197.
6. Yang, H. et al. (2021) Mol. Med. 27:58.
7. Venereau, E. et al. (2012) J. Exp. Med. 209:1519.
8. Bianchi, M.E. and A. Agresti (2005) Curr. Opin. Genet. Dev. 15:496.
9. Bonaldi, T. et al. (2003) EMBO J. 22:5551.
10. Diener, K.R. et al. (2013) Immunol. Cell Biol. 91:443.
11. Fang, P. et al. (2012) Mol. Neurobiol. 45:499.
12. de Souza, A.W. et al. (2012) Autoimmun. Rev. 11:909.
13. Bae, J.S. (2012) Arch. Pharm. Res. 35:1511.

Publications for HMGB1/HMG-1 (1690-HMB)(84)

Publications using 1690-HMB

• Zhao, Q;Zeng, C;Luo, F;Xian, Z;Wen, H;Tu, X;Yang, R;Sun, Y;Zheng, X;Xu, J;Wang, H; PDE4 inhibition alleviates HMGB1/C1q/C3-mediated excessive phagocytic pruning of synapses by microglia and depressive-like behaviors in mice Brain, behavior, and immunity 2025-02-11 [PMID: 39947489] (Bioassay, In vivo assay, Mouse)
• Saha, A;Islam, MM;Kumar, R;Ismail, AM;Garcia, E;Gullapali, RR;Chodosh, J;Rajaiya, J; Virus and Cell Specific HMGB1 Secretion and Subepithelial Infiltrate Formation in Adenovirus Keratitis bioRxiv : the preprint server for biology 2025-01-07 [PMID: 39829903] (Bioassay, Human)
• Nolfi-Donegan, D;Annarapu, GK;St Croix, C;Calderon, M;Hillery, CA;Shiva, S; High-mobility group box 1 increases platelet surface P2Y12 and platelet activation in sickle cell disease JCI insight 2024-03-08 [PMID: 38456510] (In vivo assay, Mouse)
• Tsoporis, JN;Triantafyllis, AS;Kalogeropoulos, AS;Izhar, S;Rigopoulos, AG;Rallidis, LS;Sakadakis, E;Toumpoulis, IK;Salpeas, V;Leong-Poi, H;Parker, TG;Rizos, I; Differential Expression of Circulating Damage-Associated Molecular Patterns in Patients with Coronary Artery Ectasia Biomolecules 2023-12-21 [PMID: 38275751] (Cell Culture, Human)
• Roux, AE;Yuan, H;Podshivalova, K;Hendrickson, D;Kerr, R;Kenyon, C;Kelley, D; Individual cell types in C. elegans age differently and activate distinct cell-protective responses Cell reports 2023-08-01 [PMID: 37531250] (In vivo assay, Rat)
• Pujals, M;Mayans, C;Bellio, C;Méndez, O;Greco, E;Fasani, R;Alemany-Chavarria, M;Zamora, E;Padilla, L;Mitjans, F;Nuciforo, P;Canals, F;Nonell, L;Abad, M;Saura, C;Tabernero, J;Villanueva, J; RAGE/SNAIL1 signaling drives epithelial-mesenchymal plasticity in metastatic triple-negative breast cancer Oncogene 2023-07-19 [PMID: 37468678] (Bioassay, Human)
• Overstreet, AC;Anderson, B;Burge, M;Zhu, X;Tao, Y;Cham, CM;Michaud, B;Horam, S;Sangwan, N;Dwidar, M;Liu, X;Santos, A;Finney, C;Dai, Z;Leone, VA;Messer, JS; HMGB1 acts as an agent of host defense at the gut mucosal barrier bioRxiv : the preprint server for biology 2023-05-30 [PMID: 37398239] (Bioassay, Human, Bacteria - E. Coli)
• Xu, K;Wang, M;Wang, H;Zhao, S;Tu, D;Gong, X;Li, W;Liu, X;Zhong, L;Chen, J;Xie, P; HMGB1/STAT3/p65 Axis Drives Microglial Activation and Autophagy Exert a Crucial Role in Chronic Stress-Induced Major Depressive Disorder Journal of advanced research 2023-06-13 [PMID: 37321346] (In vivo assay, Mouse)
• Zhang, Q;Chen, Y;Wang, Q;Wang, Y;Feng, W;Chai, L;Liu, J;Li, D;Chen, H;Qiu, Y;Shen, N;Shi, X;Xie, X;Li, M; HMGB1-induced activation of ER stress contributes to pulmonary artery hypertension in vitro and in vivo Respiratory research 2023-06-02 [PMID: 37268944] (Bioassay, Rat)
• Banks, WA;Hansen, KM;Erickson, MA;Crews, FT; High-mobility group box 1 (HMGB1) crosses the BBB Bidirectionally Brain, behavior, and immunity 2023-05-03 [PMID: 37146655] (In Vivo, Mouse)
• SH Kim, DE Jung, JY Song, J Jung, JK Jung, H Lee, E Roh, JT Hong, SB Han, Y Kim Targeting IKKbeta Activity to Limit Sterile Inflammation in Acetaminophen-Induced Hepatotoxicity in Mice Pharmaceutics, 2023-02-20;15(2):. 2023-02-20 [PMID: 36840032] (Bioassay, Mouse)
• J Zheng, P Lan, M Li, MC Kang, M Xun, X Ma, M Yan, D Sun, Y Shen, X Fu, X Ding, X Yan, WJ Xue Anti-Na+/K+-ATPase DR antibody attenuates UUO-induced renal fibrosis through inhibition of Na+/K+-ATPase alpha1-dependent HMGB1 release International immunopharmacology, 2023-02-09;116(0):109826. 2023-02-09 [PMID: 36764269] (Bioassay, Human)
• S Osonoi, H Mizukami, Y Takeuchi, H Sugawa, S Ogasawara, S Takaku, T Sasaki, K Kudoh, K Ito, K Sango, R Nagai, Y Yamamoto, M Daimon, H Yamamoto, S Yagihashi RAGE activation in macrophages and development of experimental diabetic polyneuropathy JCI Insight, 2022-12-08;7(23):. 2022-12-08 [PMID: 36477360] (Cell Culture, Mouse)
• S Takasawa, C Tsuchida, S Sakuramoto, T Uchiyama, M Makino, A Yamauchi, A Itaya-Hiro Upregulation of REG IV gene in human intestinal epithelial cells by lipopolysaccharide via downregulation of microRNA-24 Journal of Cellular and Molecular Medicine, 2022-08-09;0(0):. 2022-08-09 [PMID: 35946046] (Cell Culture, Human)
• CE Roth, RB Craveiro, C Niederau, H Malyaran, S Neuss, J Jankowski, M Wolf Mechanical Compression by Simulating Orthodontic Tooth Movement in an In Vitro Model Modulates Phosphorylation of AKT and MAPKs via TLR4 in Human Periodontal Ligament Cells International Journal of Molecular Sciences, 2022-07-22;23(15):. 2022-07-22 [PMID: 35897640] (Bioassay, Human)
• K Amornsupak, S Thongchot, C Thinyakul, C Box, S Hedayat, P Thuwajit, SA Eccles, C Thuwajit HMGB1 mediates invasion and PD-L1 expression through RAGE-PI3K/AKT signaling pathway in MDA-MB-231 breast cancer cells BMC Cancer, 2022-05-24;22(1):578. 2022-05-24 [PMID: 35610613] (Bioassay, Human)
• X Bi, X Yan, B Jiang, J Liang, J Zhou, S Lu, J Liu, L Luo, Z Yin Indoprofen exerts a potent therapeutic effect against sepsis by alleviating high mobility group box 1-mediated inflammatory responses Toxicology and Applied Pharmacology, 2021-10-29;433(0):115778. 2021-10-29 [PMID: 34755645] (Bioassay, Human)
• S Fischer, E Nasyrov, M Brosien, KT Preissner, HH Marti, R Kunze Self-extracellular RNA promotes pro-inflammatory response of astrocytes to exogenous and endogenous danger signals Journal of Neuroinflammation, 2021-11-02;18(1):252. 2021-11-02 [PMID: 34727934] (Bioassay, Mouse)
• Samantha M. Morrissey, Fan Zhang, Chuanlin Ding, Diego Elias Montoya-Durango, Xiaoling Hu, Chenghui Yang et al. Tumor-derived exosomes drive immunosuppressive macrophages in a pre-metastatic niche through glycolytic dominant metabolic reprogramming Cell Metabolism 2021-10-01 [PMID: 34559989] (Bioassay, Mouse)
• S Kishi, Y Nishiguchi, K Honoki, S Mori, R Fujiwara-T, T Sasaki, K Fujii, I Kawahara, K Goto, C Nakashima, A Kido, Y Tanaka, Y Luo, H Kuniyasu Role of Glycated High Mobility Group Box-1 in Gastric Cancer International Journal of Molecular Sciences, 2021-05-13;22(10):. 2021-05-13 [PMID: 34068442] (Bioassay, Human)
• A Monir, T Mukaibo, ABM Abd El-Aal, T Nodai, T Munemasa, Y Kondo, C Masaki, MA El-Shair, K Matsuo, R Hosokawa Local administration of HMGB-1 promotes bone regeneration on the critical-sized mandibular defects in rabbits Scientific Reports, 2021-04-26;11(1):8950. 2021-04-26 [PMID: 33903607] (Bioassay, Rabbit)
• M Chen, L Zhu, M Xue, R Zhu, L Jing, H Wang, Y Qin HMGB1, anti-HMGB1 antibodies, and ratio of HMGB1/anti-HMGB1 antibodies as diagnosis indicator in fever of unknown origin Scientific Reports, 2021-03-03;11(1):5059. 2021-03-03 [PMID: 33658546] (ELISA Capture, Human)
• R Chen, Y Huang, J Quan, J Liu, H Wang, TR Billiar, MT Lotze, HJ Zeh, R Kang, D Tang HMGB1 as a potential biomarker and therapeutic target for severe COVID-19 Heliyon, 2020-12-07;6(12):e05672. 2020-12-07 [PMID: 33313438] (Bioassay, Human)
• B Weber, I Lackner, M Baur, G Fois, F Gebhard, I Marzi, H Schrezenme, B Relja, M Kalbitz Effects of Circulating HMGB-1 and Histones on Cardiomyocytes-Hemadsorption of These DAMPs as Therapeutic Strategy after Multiple Trauma J Clin Med, 2020-05-11;9(5):. 2020-05-11 [PMID: 32403440] (Bioassay, Human, Mouse)
• P Wang, F Chen, X Zhang Cyclosporine-a attenuates retinal inflammation by inhibiting HMGB-1 formation in rats with type 2 diabetes mellitus BMC Pharmacol Toxicol, 2020-02-04;21(1):9. 2020-02-04 [PMID: 32019593] (In Vivo, Rat)
• Z Wang, H Zhou, H Zheng, X Zhou, G Shen, X Teng, X Liu, J Zhang, X Wei, Z Hu, F Zeng, Y Hu, J Hu, X Wang, S Chen, J Cheng, C Zhang, Y Gui, S Zou, Y Hao, Q Zhao, W Wu, Y Zhou, K Cui, N Huang, Y Wei, W Li, J Li Autophagy-based unconventional secretion of HMGB1 by keratinocytes plays a pivotal role in psoriatic skin in?ammation Autophagy, 2020-02-16;0(0):1-24. 2020-02-16 [PMID: 32019420] (In Vivo, Mouse)
• Y Le, Y Wang, L Zhou, J Xiong, J Tian, X Yang, X Gai, Y Sun Cigarette smoke-induced HMGB1 translocation and release contribute to migration and NF-kappaB activation through inducing autophagy in lung macrophages J. Cell. Mol. Med., 2019-11-26;0(0):. 2019-11-26 [PMID: 31769590] (Cell Culture, Mouse)
• FY Liu, D Fan, Z Yang, N Tang, Z Guo, SQ Ma, ZG Ma, HM Wu, W Deng, QZ Tang TLR9 is essential for HMGB1-mediated post-myocardial infarction tissue repair through affecting apoptosis, cardiac healing, and angiogenesis Cell Death Dis, 2019-06-17;10(7):480. 2019-06-17 [PMID: 31209243] (Bioassay, Mouse)
• SJ Kim, MJ Ryu, J Han, Y Jang, MJ Lee, X Ju, I Ryu, YL Lee, E Oh, W Chung, JY Heo, GR Kweon Non-cell�autonomous modulation of tyrosine hydroxylase by HMGB1 released from astrocytes in an acute MPTP-induced Parkinsonian mouse model Lab. Invest., 2019-05-01;0(0):. 2019-05-01 [PMID: 31043679] (In Vivo, Mouse)
• JH Hwang, H Chu, Y Ahn, J Kim, DY Kim HMGB1 promotes hair growth via the modulation of prostaglandin metabolism Sci Rep, 2019-04-30;9(1):6660. 2019-04-30 [PMID: 31040377] (Bioassay, Human)
• AM Nicaise, LJ Wagstaff, CM Willis, C Paisie, H Chandok, P Robson, V Fossati, A Williams, SJ Crocker Cellular senescence in progenitor cells contributes to diminished remyelination potential in progressive multiple sclerosis Proc. Natl. Acad. Sci. U.S.A., 2019-03-25;0(0):. 2019-03-25 [PMID: 30910981] (Bioassay, Rat)
• WS Yang, JJ Kim, MJ Lee, EK Lee, SK Park Ectodomain Shedding of RAGE and TLR4 as a Negative Feedback Regulation in High-Mobility Group Box 1-Activated Aortic Endothelial Cells Cell. Physiol. Biochem., 2018-11-29;51(4):1632-1644. 2018-11-29 [PMID: 30497069] (Bioassay, Human)
• K Mou, W Liu, Y Miao, F Cao, P Li HMGB1 deficiency reduces H2 O2 -induced oxidative damage in human melanocytes via the Nrf2 pathway J. Cell. Mol. Med., 2018-10-19;0(0):. 2018-10-19 [PMID: 30338917] (Bioassay, Human)
• Valentina Cecchinato, Gianluca D'Agostino, Lorenzo Raeli, Alessandra Nerviani, Milena Schiraldi, Gabriela Danelon et al. Redox-Mediated Mechanisms Fuel Monocyte Responses to CXCL12/HMGB1 in Active Rheumatoid Arthritis Frontiers in Immunology 2018-09-19 [PMID: 30283452] (Bioassay, Human)
• K Rayavara, A Kurosky, S J Stafford, NJ Garg, AR Brasier, RP Garofalo, YM Hosakote Proinflammatory Effects of Respiratory Syncytial Virus-Induced Epithelial HMGB1 on Human Innate Immune Cell Activation J. Immunol., 2018-10-01;0(0):. 2018-10-01 [PMID: 30275049] (Bioassay, Human)
• H Wu, Z Chen, JZ Chen, LG Pei, J Xie, ZH Wei, LN Kang, L Wang, B Xu High Mobility Group B-1 (HMGB-1) Promotes Apoptosis of Macrophage-Derived Foam Cells by Inducing Endoplasmic Reticulum Stress Cell. Physiol. Biochem., 2018-07-24;48(3):1019-1029. 2018-07-24 [PMID: 30041247] (Bioassay, Mouse)
• SM Bestall, RP Hulse, Z Blackley, M Swift, N Ved, K Paton, N Beazley-Lo, DO Bates, LF Donaldson Sensory neuronal sensitisation occurs through HMGB-1/ RAGE and TRPV1 in high glucose conditions J. Cell. Sci., 2018-07-26;0(0):. 2018-07-26 [PMID: 29930087] (In Vivo, Rat)
• D Senthilkum, K Rajukumar, M Kumar, S Kalaiyaras, D Shrivastav, M Katare, DD Kulkarni, VP Singh Porcine reproductive and respiratory syndrome virus induces concurrent elevation of High Mobility Group Box-1 protein and pro-inflammatory cytokines in experimentally infected piglets Cytokine, 2018-06-10;0(0):. 2018-06-10 [PMID: 29895396] (Bioassay, Porcine)
• SY Kim, M Son, SE Lee, IH Park, MS Kwak, M Han, HS Lee, ES Kim, JY Kim, JE Lee, JE Choi, B Diamond, JS Shin High-Mobility Group Box 1-Induced Complement Activation Causes Sterile Inflammation Front Immunol, 2018-04-11;9(0):705. 2018-04-11 [PMID: 29696019] (ELISA Capture, Human)
• H Mori, M Murakami, T Tsuda, K Kameda, R Utsunomiya, K Masuda, K Shiraishi, X Dai, M Tohyama, H Nakaoka, K Sayama Reduced-HMGB1 suppresses poly(I:C)-induced inflammation in keratinocytes J. Dermatol. Sci., 2018-02-02;0(0):. 2018-02-02 [PMID: 29395576] (Bioassay, Human)
• MR Dyer, Q Chen, S Haldeman, H Yazdani, R Hoffman, P Loughran, A Tsung, BS Zuckerbrau, RL Simmons, MD Neal Deep vein thrombosis in mice is regulated by platelet HMGB1 through release of neutrophil-extracellular traps and DNA Sci Rep, 2018-02-01;8(1):2068. 2018-02-01 [PMID: 29391442] (Mouse)
• MC Gruda, KG Ruggeberg, P O'Sullivan, T Guliashvil, AR Scheirer, TD Golobish, VJ Capponi, PP Chan Broad adsorption of sepsis-related PAMP and DAMP molecules, mycotoxins, and cytokines from whole blood using CytoSorb� sorbent porous polymer beads PLoS ONE, 2018-01-25;13(1):e0191676. 2018-01-25 [PMID: 29370247] (Protein Filtration, Human)
• AM Abu El-Asr, K Alam, M Garcia-Ram, A Ahmad, MM Siddiquei, G Mohammad, A Mousa, G De Hertogh, G Opdenakker, R Simó Association of HMGB1 with oxidative stress markers and regulators in PDR Mol. Vis., 2017-12-05;23(0):853-871. 2017-12-05 [PMID: 29259392] (In Vivo, Rat)
• SH Park, JA Kwak, SH Jung, B Ahn, WJ Cho, CY Yun, CS Na, BY Hwang, JT Hong, SB Han, Y Kim Piperidylmethyloxychalcone improves immune-mediated acute liver failure via inhibiting TAK1 activity Exp. Mol. Med., 2017-11-17;49(11):e392. 2017-11-17 [PMID: 29147012] (Bioassay, Mouse)
• F Zhang, X Su, G Huang, XF Xin, EH Cao, Y Shi, Y Song sRAGE alleviates neutrophilic asthma by blocking HMGB1/RAGE signalling in airway dendritic cells Sci Rep, 2017-10-27;7(1):14268. 2017-10-27 [PMID: 29079726] (Bioassay, Mouse)
• J Zhu, FL Wang, HB Wang, N Dong, XM Zhu, Y Wu, YT Wang, YM Yao TNF-? mRNA is negatively regulated by microRNA-181a-5p in maturation of dendritic cells induced by high mobility group box-1 protein Sci Rep, 2017-09-25;7(1):12239. 2017-09-25 [PMID: 28947753] (Bioassay, Mouse)
• L Ding, JA Buckwalter, JA Martin DAMPs Synergize with Cytokines or Fibronectin Fragment on Inducing Chondrolysis but Lose Effect When Acting Alone Mediators Inflamm., 2017-07-18;2017(0):2642549. 2017-07-18 [PMID: 28804219] (Bioassay, Human)
• H Deng, C Wang, DY Chang, N Hu, M Chen, MH Zhao High mobility group box-1 contributes to anti-myeloperoxidase antibody-induced glomerular endothelial cell injury through a moesin-dependent route Arthritis Res. Ther., 2017-06-06;19(1):125. 2017-06-06 [PMID: 28587670] (Bioassay, Human)
• OJ Taylor, MO Thatcher, ST Carr, JL Gibbs, AM Trumbull, ME Harrison, DR Winden, MJ Pearson, TS Tippetts, WL Holland, PR Reynolds, BT Bikman High-Mobility Group Box 1 Disrupts Metabolic Function with Cigarette Smoke Exposure in a Ceramide-Dependent Manner Int J Mol Sci, 2017-05-20;18(5):. 2017-05-20 [PMID: 28531105] (In Vivo, Mouse)
• Z Li, EK Fan, J Liu, MJ Scott, Y Li, S Li, W Xie, TR Billiar, MA Wilson, Y Jiang, P Wang, J Fan Cold-inducible RNA-binding protein through TLR4 signaling induces mitochondrial DNA fragmentation and regulates macrophage cell death after trauma Cell Death Dis, 2017-05-11;8(5):e2775. 2017-05-11 [PMID: 28492546] (Bioassay, Mouse)
• F Zhao, Y Fang, S Deng, X Li, Y Zhou, Y Gong, H Zhu, W Wang Glycyrrhizin Protects Rats from Sepsis by Blocking HMGB1 Signaling Biomed Res Int, 2017-04-18;2017(0):9719647. 2017-04-18 [PMID: 28484719] (Bioassay, Rat)
• L Di Candia, E Gomez, E Venereau, L Chachi, D Kaur, ME Bianchi, RA Challiss, CE Brightling, RM Saunders HMGB1 is upregulated in the airways in asthma and potentiates airway smooth muscle contraction via TLR4 J. Allergy Clin. Immunol, 2017-03-01;0(0):. 2017-03-01 [PMID: 28259445] (Bioassay, Mouse)
• C Wang, DY Chang, M Chen, MH Zhao HMGB1 contributes to glomerular endothelial cell injury in ANCA-associated vasculitis through enhancing endothelium-neutrophil interactions J. Cell. Mol. Med, 2017-02-09;0(0):. 2017-02-09 [PMID: 28181422] (Bioassay, Human)
• CD16 is indispensable for antibody-dependent cellular cytotoxicity by human monocytes Sci Rep, 2016-09-27;6(0):34310. 2016-09-27 [PMID: 27670158] (Bioassay, Human)
• Cerebral ischemic post?conditioning induces autophagy inhibition and a HMGB1 secretion attenuation feedback loop to protect against ischemia reperfusion injury in an oxygen glucose deprivation cellular model Mol Med Rep, 2016-09-19;0(0):. 2016-09-19 [PMID: 27666823] (Bioassay, Rat)
• Hemorrhagic shock primes for lung vascular endothelial cell pyroptosis: role in pulmonary inflammation following LPS Cell Death Dis, 2016-09-08;7(9):e2363. 2016-09-08 [PMID: 27607578] (Bioassay, Mouse)
• High Mobility Group Box Protein 1 Boosts Endothelial Albumin Transcytosis through the RAGE/Src/Caveolin-1 Pathway Sci Rep, 2016-08-30;6(0):32180. 2016-08-30 [PMID: 27572515] (Bioassay, Mouse)
• Receptor for advanced glycation endproducts signaling cascades are activated in pancreatic fibroblasts, but not in the INS1E insulinoma cell line: Are mesenchymal cells major players in chronic inflammation? Islets, 2016-07-14;0(0):0. 2016-07-14 [PMID: 27415824] (Bioassay, Rat)
• Qiang Chen HMGB1 Induces Secretion of Matrix Vesicles by Macrophages to Enhance Ectopic Mineralization PLoS ONE, 2016-05-31;11(5):e0156686. 2016-05-31 [PMID: 27243975] (Bioassay, Mouse)
• Dyer , Kimberly, Rosenberg , Helene F Physiologic concentrations of HMGB1 have no impact on cytokine-mediated eosinophil survival or chemotaxis in response to Eotaxin-2 (CCL24). PLoS ONE, 2015-03-16;10(3):e0118887. 2015-03-16 [PMID: 25774667] (Bioassay, Human, Mouse)
• Lee C, Wang C, Lee Y, Tsai Y, Liu J High mobility group box 1 induced human lung myofibroblasts differentiation and enhanced migration by activation of MMP-9. PLoS ONE, 2015-02-18;10(2):e0116393. 2015-02-18 [PMID: 25692286] (Bioassay, Human)
• Vogel S, Borger V, Peters C, Forster M, Liebfried P, Metzger K, Meisel R, Daubener W, Trapp T, Fischer J, Gawaz M, Sorg R Necrotic cell-derived high mobility group box 1 attracts antigen-presenting cells but inhibits hepatocyte growth factor-mediated tropism of mesenchymal stem cells for apoptotic cell death. Cell Death Differ, 2015-01-09;22(7):1219-30. 2015-01-09 [PMID: 25571972] (Bioassay, Mouse)
• Park S, Lee S, Kim H, Lee W, Hong K, Kim C HMGB1 induces angiogenesis in rheumatoid arthritis via HIF-1alpha activation. Eur J Immunol, 2015-01-15;45(4):1216-27. 2015-01-15 [PMID: 25545169] (Bioassay, Human)
• Yang P, Kim D, Lee Y, Lee S, Kang W, Chang H, Shin J Glycyrrhizin, inhibitor of high mobility group box-1, attenuates monocrotaline-induced pulmonary hypertension and vascular remodeling in rats. Respir Res, 2014-11-25;15(1):148. 2014-11-25 [PMID: 25420924] (Bioassay, Human)
• Kim H, Park S, Lee S, Lee H, Lee W, Rhim B, Hong K, Kim C Inhibition of HMGB1-induced angiogenesis by cilostazol via SIRT1 activation in synovial fibroblasts from rheumatoid arthritis. PLoS ONE, 2014-08-15;9(8):e104743. 2014-08-15 [PMID: 25126750] (Bioassay, Human)
• Zhou H, Ji X, Wu Y, Xuan J, Qi Z, Shen L, Lan L, Li Q, Yin Z, Li Z, Zhao Z A dual-role of Gu-4 in suppressing HMGB1 secretion and blocking HMGB1 pro-inflammatory activity during inflammation. PLoS ONE, 2014-03-06;9(3):e89634. 2014-03-06 [PMID: 24603876] (Bioassay, Human)
• Thierry A, Giraud S, Robin A, Barra A, Bridoux F, Ameteau V, Hauet T, Girard J, Touchard G, Gombert J, Herbelin A The alarmin concept applied to human renal transplantation: evidence for a differential implication of HMGB1 and IL-33. PLoS ONE, 2014-02-20;9(2):e88742. 2014-02-20 [PMID: 24586382] (Bioassay, Human)
• Vogel S, Chatterjee M, Metzger K, Borst O, Geisler T, Seizer P, Muller I, Mack A, Schumann S, Buhring H, Lang F, Sorg R, Langer H, Gawaz M Activated platelets interfere with recruitment of mesenchymal stem cells to apoptotic cardiac cells via high mobility group box 1/Toll-like receptor 4-mediated down-regulation of hepatocyte growth factor receptor MET. J Biol Chem, 2014-02-24;289(16):11068-82. 2014-02-24 [PMID: 24567328] (Bioassay, Human)
• Santos , Andrea R, Dvoriantchikova , Galina, Li , Yiwen, Mohammad , Ghulam, Abu El-Asrar , Ahmed M, Wen , Rong, Ivanov , Dmitry Cellular mechanisms of high mobility group 1 (HMGB-1) protein action in the diabetic retinopathy. PLoS ONE, 2014-01-31;9(1):e87574. 2014-01-31 [PMID: 24498140] (Bioassay, In Vivo, Human, Mouse)
• Luo , Yi, Yoneda , Junya, Ohmori , Hitoshi, Sasaki , Takamits, Shimbo , Kazutaka, Eto , Sachise, Kato , Yumiko, Miyano , Hiroshi, Kobayashi , Tsuyoshi, Sasahira , Tomonori, Chihara , Yoshitom, Kuniyasu , Hiroki Cancer usurps skeletal muscle as an energy repository. Cancer Res, 2013-11-06;74(1):330-40. 2013-11-06 [PMID: 24197136] (Bioassay, Mouse)
• Suwara M, Green N, Borthwick L, Mann J, Mayer-Barber K, Barron L, Corris P, Farrow S, Wynn T, Fisher A, Mann D IL-1alpha released from damaged epithelial cells is sufficient and essential to trigger inflammatory responses in human lung fibroblasts. Mucosal Immunol, 2013-10-30;7(3):684-93. 2013-10-30 [PMID: 24172847] (ELISA (Standard), Human)
• Ahmed M. Abu El-Asrar, Mohd Imtiaz Nawaz, Mohammad Mairaj Siddiquei, Abdullah S. Al-Kharashi, Dustan Kangave, Ghulam Mohammad High-Mobility Group Box-1 Induces Decreased Brain-Derived Neurotrophic Factor-Mediated Neuroprotection in the Diabetic Retina Mediators of Inflammation 2013-05-20 [PMID: 23766563] (In vivo assay, Rat)
• Chen , Jianlin, Matzuk , Martin M, Zhou , Xin J, Lu , Christop Endothelial pentraxin 3 contributes to murine ischemic acute kidney injury. Kidney Int, 2012-08-15;82(11):1195-207. 2012-08-15 [PMID: 22895517] (Bioassay, Human)
• The persistent release of HMGB1 contributes to tactile hyperalgesia in a rodent model of neuropathic pain. J Neuroinflammation, 2012-07-23;9(0):180. 2012-07-23 [PMID: 22824385] (Bioassay, Rat)
• S100P-derived RAGE antagonistic peptide reduces tumor growth and metastasis. Clin. Cancer Res., 2012-06-20;18(16):4356-64. 2012-06-20 [PMID: 22718861] (Binding Assay, Human)
• Sakaguchi M, Murata H, Yamamoto K, Ono T, Sakaguchi Y, Motoyama A, Hibino T, Kataoka K, Huh NH TIRAP, an adaptor protein for TLR2/4, transduces a signal from RAGE phosphorylated upon ligand binding. PLoS ONE, 2011-08-01;6(8):e23132. 2011-08-01 [PMID: 21829704] (Bioassay, Human)
• Ley C, Svala E, Nilton A, Lindahl A, Eloranta ML, Ekman S, Skioldebrand E Effects of high mobility group box protein-1, interleukin-1beta, and interleukin-6 on cartilage matrix metabolism in three-dimensional equine chondrocyte cultures. Connect. Tissue Res., 2010-11-30;52(4):290-300. 2010-11-30 [PMID: 21117899] (Bioassay, Equine)
• Ruan BH, Li X, Winkler AR, Cunningham KM, Kuai J, Greco RM, Nocka KH, Fitz LJ, Wright JF, Pittman DD, Tan XY, Paulsen JE, Lin LL, Winkler DG Complement C3a, CpG oligos, and DNA/C3a complex stimulate IFN-alpha production in a receptor for advanced glycation end product-dependent manner. J. Immunol., 2010-09-03;185(7):4213-22. 2010-09-03 [PMID: 20817881] (Binding Assay, Human)
• Rao NV, Argyle B, Xu X, Reynolds PR, Walenga JM, Prechel M, Prestwich GD, MacArthur RB, Walters BB, Hoidal JR, Kennedy TP Low anticoagulant heparin targets multiple sites of inflammation, suppresses heparin-induced thrombocytopenia, and inhibits interaction of RAGE with its ligands. Am. J. Physiol., Cell Physiol., 2010-04-07;299(1):C97-110. 2010-04-07 [PMID: 20375277] (Binding Assay, ELISA (Capture), In Vivo, Human, Mouse)
• Li Y, Xiang M, Yuan Y, Xiao G, Zhang J, Jiang Y, Vodovotz Y, Billiar TR, Wilson MA, Fan J Hemorrhagic shock augments lung endothelial cell activation: role of temporal alterations of TLR4 and TLR2. Am. J. Physiol. Regul. Integr. Comp. Physiol., 2009-10-14;297(6):R1670-80. 2009-10-14 [PMID: 19828841] (Bioassay, Mouse)
• Ghiringhelli F, Apetoh L, Tesniere A, Aymeric L, Ma Y, Ortiz C, Vermaelen K, Panaretakis T, Mignot G, Ullrich E, Perfettini JL, Schlemmer F, Tasdemir E, Uhl M, Genin P, Civas A, Ryffel B, Kanellopoulos J, Tschopp J, Andre F, Lidereau R, McLaughlin NM, Haynes NM, Smyth MJ, Kroemer G, Zitvogel L Activation of the NLRP3 inflammasome in dendritic cells induces IL-1beta-dependent adaptive immunity against tumors. Nat. Med., 2009-09-20;15(10):1170-8. 2009-09-20 [PMID: 19767732] (Bioassay, Mouse)
• Zhou J, Huang JD, Poon VK, Chen DQ, Chan CC, Ng F, Guan XY, Watt RM, Lu L, Yuen KY, Zheng BJ Functional dissection of an IFN-alpha/beta receptor 1 promoter variant that confers higher risk to chronic hepatitis B virus infection. J. Hepatol., 2009-05-03;51(2):322-32. 2009-05-03 [PMID: 19501422] (Western Blot)
• Tzeng HP, Fan J, Vallejo JG, Dong JW, Chen X, Houser SR, Mann DL Negative inotropic effects of high-mobility group box 1 protein in isolated contracting cardiac myocytes. Am. J. Physiol. Heart Circ. Physiol., 2008-01-25;294(3):H1490-6. 2008-01-25 [PMID: 18223193] (Bioassay, Feline)
• Kiryushko D, Novitskaya V, Soroka V, Klingelhofer J, Lukanidin E, Berezin V, Bock E Molecular mechanisms of Ca(2+) signaling in neurons induced by the S100A4 protein. Mol. Cell. Biol., 2006-05-01;26(9):3625-38. 2006-05-01 [PMID: 16612001] (Surface Plasmon Resonance, Human)

Bioinformatics

• Gene Symbol: HMGB1
• Uniprot:
  • P09429()