Recombinant Human His6-USP4 Protein, CF

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human His6-USP4 Protein, CF Summary

• Details of Functionality: Reaction conditions will need to be optimized for each specific application. We recommend an initial recombinant human USP4 concentration of 10-100 nM when using Ubiquitin-AMC or Ubiquitin-Rh110 substrates (U-550, U-555)
• Source: Spodoptera frugiperda, Sf 21 (baculovirus)-derived human USP4 protein Met1 - Asn963 with a C-terminal 6-His tag
• Accession #: Q13107.3
• Protein/Peptide Type: Recombinant Enzymes
• Gene: USP4
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 109 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  6 months from date of receipt, -70 degreesC as supplied. 3 months, -70 degreesC under sterile conditions after opening.
• Buffer: Supplied as a solution in HEPES, NaCl, TCEP and Glycerol.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human His6-USP4 Protein, CF

• Deubiquitinating enzyme 4
• EC 3.1.2.15
• EC 3.4.19.12
• MGC149848
• MGC149849
• ubiquitin carboxyl-terminal esterase 4
• ubiquitin carboxyl-terminal hydrolase 4
• ubiquitin specific peptidase 4 (proto-oncogene)
• ubiquitin specific protease 4 (proto-oncogene)
• ubiquitin thioesterase 4
• Ubiquitin thiolesterase 4
• ubiquitin-specific processing protease 4
• Ubiquitin-specific-processing protease 4
• Ubiquitous nuclear protein homolog
• UNP
• Unph
• UNPUnph
• USP4

Background

Ubiquitin carboxyl-terminal hydrolase 4 (USP4) is a specialized cysteine protease with a predicted molecular weight of 109 kDa. USP4 is a member of the peptidase C19 family and the human protein shares 91% amino acid sequence identity with its mouse ortholog. USP4 is ubiquitinated on multiple sites, and deubiquitination of these sites requires USP4 catalytic activity. USP4 interacts with CtIP protein and the MRN (MRE11-RAD50-NBS1) complex to promote DNA double-strand break repair. Other reported USP4 targets include HDAC2, ADORA2A, PDPK1 and TRIM21.

1. Li, Z., et al. (2016) Oncogene 35:2902.
2. Milojevic, T., et al. (2006) Mol. Pharmacol. 69:1083.
3. Wada, K. & T. Kamitani (2006) Biochem. Biophys. Res. Commun. 342:253.
4. Wijnhoven, P., et al. (2015) Mol. Cell 60:362.

Bioinformatics

• Gene Symbol: USP4
• Uniprot:
  • Q13107.3()