Recombinant Human Glypican 6 Protein, CF

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Binding Activity
Format
Carrier-Free

Order Details

Recombinant Human Glypican 6 Protein, CF Summary

Details of Functionality
Measured by its binding ability in a functional ELISA. When biotinylated recombinant mouse Wnt-3a (25 ng/mL, 100 μL/well) is captured on streptavidin-coated plate, Recombinant Human Glypican 6 binds with an apparent Kd <10 nM.
Source
Mouse myeloma cell line, NS0-derived human Glypican 6 protein
Asp24-Arg354 & Ser355-Val527, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Asp24 & Ser355
Protein/Peptide Type
Recombinant Proteins
Gene
GPC6
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Binding Activity
Theoretical MW
38 kDa and 20 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
38 kDa and 27-29 kDa, reducing conditions
Publications
Read Publications using
2845-GP in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Glypican 6 Protein, CF

  • Glypican 6
  • glypican proteoglycan 6
  • glypican-6
  • GPC6
  • MGC126288
  • OMIMD1

Background

The Glypicans (glypiated proteoglycans) are a small multigene family of GPI-linked heparan sulfate (HS) proteoglycans that likely play a key role in embryonic morphogenesis (1, 2, 3, 4). There are currently six known mammalian Glypicans. They all share a common-sized protein core of 60 - 70 kDa, an N-terminus which likely forms a compact globular domain, 14 conserved cysteines that form multiple intrachain disulfide bonds, and a number of C-terminal N- and O-linked carbohydrate attachment sites. Based on exon organization and the location of O-linked glycosylation sites, at least two subfamilies of Glypicans are known, with one subfamily containing Glypicans 1, 2, 4 and 6, and another subfamily containing Glypicans 3 and 5 (3, 5). Human Glypican 6 (GPC-6) is synthesized as a 554 amino acid (aa) preproprecursor that contains a 23 aa signal sequence, a 505 aa mature region and a 26 aa C-terminal prosegment (5, 6). There are four consecutive Ser-Gly repeats that serve as a heparin sulfate attachment site. GPC-6 is reported to be as large as 110 kDa in size. This translates into approximately 50 kDa of proteoglycan (5). Human to mouse, there is 97% aa identity over the entire GPC-6 molecule. Cells known to express GPC-6 are adult ovary and embryonic vascular and visceral smooth muscle, plus mesenchyme (embryonic connective tissue) in multiple organs (1, 5, 6). The function of GPC-6 is essentially unknown. As a Glypican family member, it may facilitate heparin-binding growth factor signaling and polyamine uptake into expressing cells (7, 8). In this regard, it would appear that GPC-6 with its attendant HS is downregulated by triiodothyronine during cartilage maturation, thus limiting the availability of sites for FGF sequestration and activity (9).

  1. Song, H.H. and J. Filmus (2002) Biochim. Biophys. Acta 1573:241. 
  2. Filmus, J. (2001) Glycobiology 11:19R. 
  3. De Cat, B. and G. David (2001) Semin. Cell Dev. Biol. 12:117. 
  4. Filmus, J. (2003) Glycoconj. J. 19:319. 
  5. Veugelers, M. et al. (1999) J. Biol. Chem. 274:26968.
  6. Paine-Saunders, S. et al. (1999) Genomics 57:455.
  7. Fransson, L-A. et al. (2004) Cell Mol. Life Sci. 61:1016.
  8. Fransson, L-A. (2003) Int. J. Biochem. Cell Biol. 35:125.
  9. Bassett, J.H.D. et al. (2006) Endocrinology 147:295.

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Publications for Glypican 6 (2845-GP)(3)

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Bioinformatics

Gene Symbol GPC6
Uniprot