Recombinant Human Glypican 6 Protein, CF

• Reactivity: Hu
• Applications: Binding Activity
• Format: Carrier-Free

Recombinant Human Glypican 6 Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. When biotinylated recombinant mouse Wnt-3a (25 ng/mL, 100 μL/well) is captured on streptavidin-coated plate, Recombinant Human Glypican 6 binds with an apparent K_d <10 nM.
• Source: Mouse myeloma cell line, NS0-derived human Glypican 6 protein
  Asp24-Arg354 & Ser355-Val527, with a C-terminal 6-His tag
• Accession #: Q9Y625
• N-terminal Sequence: Asp24 & Ser355
• Protein/Peptide Type: Recombinant Proteins
• Gene: GPC6
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Binding Activity
• Theoretical MW: 38 kDa and 20 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 38 kDa and 27-29 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Glypican 6 Protein, CF

• Glypican 6
• glypican proteoglycan 6
• glypican-6
• GPC6
• MGC126288
• OMIMD1

Background

The Glypicans (glypiated proteoglycans) are a small multigene family of GPI-linked heparan sulfate (HS) proteoglycans that likely play a key role in embryonic morphogenesis (1, 2, 3, 4). There are currently six known mammalian Glypicans. They all share a common-sized protein core of 60 - 70 kDa, an N-terminus which likely forms a compact globular domain, 14 conserved cysteines that form multiple intrachain disulfide bonds, and a number of C-terminal N- and O-linked carbohydrate attachment sites. Based on exon organization and the location of O-linked glycosylation sites, at least two subfamilies of Glypicans are known, with one subfamily containing Glypicans 1, 2, 4 and 6, and another subfamily containing Glypicans 3 and 5 (3, 5). Human Glypican 6 (GPC-6) is synthesized as a 554 amino acid (aa) preproprecursor that contains a 23 aa signal sequence, a 505 aa mature region and a 26 aa C-terminal prosegment (5, 6). There are four consecutive Ser-Gly repeats that serve as a heparin sulfate attachment site. GPC-6 is reported to be as large as 110 kDa in size. This translates into approximately 50 kDa of proteoglycan (5). Human to mouse, there is 97% aa identity over the entire GPC-6 molecule. Cells known to express GPC-6 are adult ovary and embryonic vascular and visceral smooth muscle, plus mesenchyme (embryonic connective tissue) in multiple organs (1, 5, 6). The function of GPC-6 is essentially unknown. As a Glypican family member, it may facilitate heparin-binding growth factor signaling and polyamine uptake into expressing cells (7, 8). In this regard, it would appear that GPC-6 with its attendant HS is downregulated by triiodothyronine during cartilage maturation, thus limiting the availability of sites for FGF sequestration and activity (9).

1. Song, H.H. and J. Filmus (2002) Biochim. Biophys. Acta 1573:241. 
2. Filmus, J. (2001) Glycobiology 11:19R. 
3. De Cat, B. and G. David (2001) Semin. Cell Dev. Biol. 12:117. 
4. Filmus, J. (2003) Glycoconj. J. 19:319. 
5. Veugelers, M. et al. (1999) J. Biol. Chem. 274:26968.
6. Paine-Saunders, S. et al. (1999) Genomics 57:455.
7. Fransson, L-A. et al. (2004) Cell Mol. Life Sci. 61:1016.
8. Fransson, L-A. (2003) Int. J. Biochem. Cell Biol. 35:125.
9. Bassett, J.H.D. et al. (2006) Endocrinology 147:295.

Publications for Glypican 6 (2845-GP)(5)

Publications using 2845-GP

• N Li, A Quan, D Li, J Pan, H Ren, G Hoeltzel, N de Val, D Ashworth, W Ni, J Zhou, S Mackay, SM Hewitt, R Cachau, M Ho The IgG4 hinge with CD28 transmembrane domain improves VHH-based CAR T cells targeting a membrane-distal epitope of GPC1 in pancreatic cancer Nature Communications, 2023-04-08;14(1):1986. 2023-04-08 [PMID: 37031249] (ELISA Capture, Human)
• C Colin-Pier, V Untereiner, GD Sockalingu, L Ramont, S Brézillon Investigation of Glypican-4 and -6 by Infrared Spectral Imaging during the Hair Growth Cycle International Journal of Molecular Sciences, 2023-02-21;24(5):. 2023-02-21 [PMID: 36901723] (Infrared Spectral Imaging, N/A)
• Wu Z, Person A, Anderson M, Burroughs B, Tatge T, Khatri K, Zou Y, Wang L, Geders T, Zaia J, Sackstein R Imaging specific cellular glycan structures using glycosyltransferases via click chemistry. Glycobiology, 2018-02-01;0(0):. 2018-02-01 [PMID: 29186441] (Bioassay)
• Glypican 6 Enhances N-Methyl-D-Aspartate Receptor Function in Human-Induced Pluripotent Stem Cell-Derived Neurons Front Cell Neurosci, 2016-11-15;10(0):259. 2016-11-15 [PMID: 27895553] (Bioassay, Human)
• Epitope mapping by a Wnt-blocking antibody: evidence of the Wnt binding domain in heparan sulfate Sci Rep, 2016-05-17;6(0):26245. 2016-05-17 [PMID: 27185050] (ELISA (Standard), Human)

Bioinformatics

• Gene Symbol: GPC6
• Uniprot:
  • Q9Y625()