Recombinant Human Glutathione Reductase Protein, CF

Images

 
There are currently no images for Glutathione Reductase (8866-GR).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Enzyme Activity
Format
Carrier-Free

Order Details

Recombinant Human Glutathione Reductase Protein, CF Summary

Details of Functionality
Measured by its ability to reduce oxidized glutathione to glutathione in an NADPH-dependent manner. The specific activity is >15,000 pmol/min/μg, as measured under the described conditions.
Source
E. coli-derived human Glutathione Reductase protein
Met44-Arg522, with an N-terminal Met and 6-His tag
Accession #
N-terminal Sequence
Met
Protein/Peptide Type
Recombinant Proteins
Gene
GSR
Purity
>85%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane.
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Enzyme Activity
Theoretical MW
53 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
53 kDa, reducing conditions

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.
Buffer
Supplied as a 0.2 μm filtered solution in Tris, NaCl, DTT and Glycerol.
Purity
>85%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane.
Assay Procedure
  • Assay Buffer: 0.1 M Tris, pH 7.5
  • Recombinant Human Glutathione Reductase (rhGSR) (Catalog # 8866-GR)
  • NADPH (Sigma, Catalog # N7505), 10 mM stock in deionized water
  • Oxidized Glutathione (Amresco, Catalog # 0524), 50 mM stock in deionized water
  • 96-well Clear Plate (Catalog # DY990)
  • Plate Reader (Model: SpectraMax Plus by Molecular Devices) or equivalent
  1. Create Substrate Mixture containing 0.2 mM NADPH and 1 mM Oxidized Glutathione in Assay Buffer.
  2. Dilute rhGSR to 0.4 µg/mL in Assay Buffer.
  3. Load 50 µL of the 0.4 µg/mL rhGSR to the plate, and start the reaction by adding 50 µL of Substrate Mixture. Include a Substrate Blank containing 50 µL of Assay Buffer and 50 µL of Substrate Mixture.
  4. Read plate in kinetic mode for 5 minutes at an absorbance of 340 nm.
  5. The specific activity can be calculated using the following equation:

     Specific Activity (pmol/min/µg) =

Adjusted Vmax* (OD/min) x -1 x well volume (L) x 1012 pmol/mol
ext. coeff** (M-1cm-1) x path corr.*** (cm) x amount of enzyme (µg)

      
      *Adjusted for Substrate blank
      **Using the extinction coefficient 6220 M-1cm-1
      ***Using the path correction 0.32 cm
      Note: the output of many spectrophotometers is in mOD.

Per Well:
  • rhGSR: 0.02 µg
  • NADPH: 0.1 mM
  • Oxidized Glutathione: 0.5 mM

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Glutathione Reductase Protein, CF

  • EC 1.8.1
  • EC 1.8.1.7
  • GLUR
  • Glutathione Reductase
  • glutathione reductase, mitochondrial
  • GR
  • GRase
  • GRD1
  • GSR
  • HEL-752
  • MGC78522

Background

Glutathione Reductase (GR) is a homodimeric flavoprotein disulfide oxidoreductase that plays a critical role in the prevention of oxidative damage within the cell (1, 2). Glutathione Reductase helps to maintain appropriate levels of intracellular reduced glutathione (GSH) which is essential for maintaining the normal structure of red blood cells and for keeping hemoglobin in the ferrous state (3). Glutathione Reductase together with its co-factor, NADPH, catalyzes the reduction of oxidized glutathione (glutathione disulfide, GSSG) to glutathione. GSH is also a reactant for Glutathione Peroxidase, which converts hydrogen peroxide hydrogen peroxide (H2O2) into water (4). Glutathione Reductase is expressed as both mitochondrial and cytosolic isoforms, as determined by the presence or absence of an N-terminal transit peptide. Human Glutathione Reductase shares 86% and 76% amino acid sequence identity with mouse and rat Glutathione Reductase, respectively (5, 6). Alternative splicing of human Glutathione Reductase generates additional isoforms with deletions in the central pyridine nucleotide-disulfide oxidoreductase domain (7).
  1. Deponte, M. (2013) Biochim. Biophys. Acta 1830:3217.
  2. Fujii, J. et al. (2011) J. Clin. Biochem. Nutr. 49:70.
  3. Tsantes, A.E. et al. (2006) Antioxid. Redox Signal. 8:1205.
  4. Lubos, E. et al. (2011) Antioxid. Redox Signal. 15:1957.
  5. Tutic, M. et al. (1990) Eur. J. Biochem. 188:523.
  6. Krauth-Siegel, R.L. et al. (1982) Eur. J. Biochem. 121:259.
  7. Satoh, N. et al. (2010) Biochem. Genet. 48:816.

Customers Who Viewed This Item Also Viewed...

AF3398
Species: Hu, Mu, Rt
Applications: ICC, KO, Simple Western, WB
NB300-731
Species: Gp, Hu, Mu, Rb, Rt, Sh, Ye
Applications: B/N, ChIP, Flow, GS, ICC/IF, IHC, IHC-P, IP, WB
NBP2-24915
Species: Bv, Hu, Mu, Rt
Applications: IHC, IHC-P, WB
NBP1-86616
Species: Hu
Applications: IHC, IHC-P, WB
NBP2-58917
Species: Hu
Applications: IHC, IHC-P, WB
NBP2-16686
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
NBP2-49118
Species: Hu
Applications: IHC, IHC-P
NB100-236
Species: Hu, Mu
Applications: IB, IHC, IHC-Fr, IP, WB
NB100-1533
Species: Hu, Mu, Po, Rt, Sh
Applications: Flow, ICC/IF, IHC, IHC-P, PEP-ELISA, WB
210-TA
Species: Hu
Applications: BA
NB300-562
Species: Ch, Hu, Mu, Rb, Rt, Sh
Applications: B/N, Flow, ICC/IF, IHC, IHC-P, WB
NB110-61574
Species: Bv, Gt, Hu, Mu, Sh
Applications: WB
NB100-116
Species: Hu, Mu, Pm, Rt
Applications: ChIP, ChIP, ELISA, GS, IB, ICC/IF, IHC, IHC-Fr, IHC-P, IP, KD, PLA, Simple Western, WB
AF5415
Species: Hu
Applications: ICC, IHC, Simple Western, WB
MAB8930
Species: Hu
Applications: ICC, Simple Western, WB
7570-GH
Species: Hu
Applications: EnzAct
NB100-56603
Species: Ca, Hu, Mu, Pm, Rt
Applications: IHC, IHC-P, WB
8866-GR
Species: Hu
Applications: Enzyme Activity

Publications for Glutathione Reductase (8866-GR) (0)

There are no publications for Glutathione Reductase (8866-GR).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Glutathione Reductase (8866-GR) (0)

There are no reviews for Glutathione Reductase (8866-GR). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

FAQs for Glutathione Reductase (8866-GR) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional Glutathione Reductase Products

Bioinformatics Tool for Glutathione Reductase (8866-GR)

Discover related pathways, diseases and genes to Glutathione Reductase (8866-GR). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Glutathione Reductase (8866-GR)

Discover more about diseases related to Glutathione Reductase (8866-GR).
 

Pathways for Glutathione Reductase (8866-GR)

View related products by pathway.

PTMs for Glutathione Reductase (8866-GR)

Learn more about PTMs related to Glutathione Reductase (8866-GR).

Blogs on Glutathione Reductase

There are no specific blogs for Glutathione Reductase, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Recombinant Human Glutathione Reductase Protein, CF and receive a gift card or discount.

Bioinformatics

Gene Symbol GSR
Uniprot