Recombinant Human GFR alpha-3 Fc Chimera Protein, CF

• Reactivity: Hu
• Applications: Binding Activity
• Format: Carrier-Free

Recombinant Human GFR alpha-3 Fc Chimera Protein, CF Summary

• Details of Functionality: Binding of R&D Systems’ human GFR alpha -3/Fc Chimera to immobilized Artemin has been reported. Baloh, R. et al. (1998) Neuron 21:1291. Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human Artemin (Catalog # 2589-AR) at 1 µg/mL can bind Recombinant Human GFR alpha ‑3/GDNF R alpha ‑3 Fc Chimera with an apparent K_d <1 nM.
• Source: Spodoptera frugiperda, Sf 21 (baculovirus)-derived human GFR alpha-3/GDNF R alpha-3 protein
  

  Human GFR alpha -3 (Gly31-Trp382) Accession # AAC24355	IEGRMD	Human IgG1 (Pro100-Lys330)	6 His tag
  Human GFR alpha -3 (Asp32-Trp382) Accession # AAC24355	IEGRMD	Human IgG1 (Pro100-Lys330)	6 His tag
  N-terminus			C-terminus

• Accession #: AAC24355
• N-terminal Sequence: Gly31 & Asp32
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Gene: GFRA3
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Binding Activity
  • Binding Activity2
• Theoretical MW: 67 kDa (monomer).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 67 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human GFR alpha-3 Fc Chimera Protein, CF

• GDNF family receptor alpha 3
• GDNF family receptor alpha-3
• GDNF R alpha-3
• GDNF receptor alpha-3
• GDNFR-alpha-3
• GFR alpha3
• GFR alpha-3
• GFRA3
• GFRa-3
• GFR-alpha-3
• glial cell line-derived neurotrophic factor receptor alpha-3
• GPI-linked receptor

Background

Human glial cell line-derived neurotrophic factor (GDNF) family receptor alpha 3 (GFR alpha -3, GDNF R alpha ‑3) is an approximately 50 kDa plasma membrane glycoprotein that is one of four GDNF receptors, all of which are glycosylphosphoinositol (GPI)‑anchored, contain three conserved cysteine repeats, and promote survival of neurons (1‑4). It is synthesized as a 400 aa precursor with a 31 aa N‑terminal signal sequence, a 343 aa mature segment, and a C-terminal GPI attachment signal sequence (3‑5). Human GFR alpha -3 shares 81‑84% aa sequence identity with mouse, rat, equine, bovine and canine GFR alpha -3. GFR alpha -3 is expressed in the central nervous system only during the earliest stages of neurogenesis, while later it is predominantly found in developing and adult nociceptive sensory neurons (2, 4‑8). It is expressed with the shared GDNF co‑receptor, the Ret receptor tyrosine kinase, in the trigeminal ganglion, pituitary gland, thymus, lung, and duodenum (2, 4). Its ligand, the GDNF family ligand artemin, is primarily expressed by immature Schwann cells in the peripheral nervous system, and vascular smooth muscle cells, directing axonal projection of sympathetic neurons (6, 7). Artemin first binds GFR alpha -3, which recruits Ret, forming a signaling complex that is a pentamer containing one artemin, two GFR alpha -3, and two Ret molecules (1, 6, 9). Signals from this complex are required for the development and survival of the superior cervical ganglion (SCG) neurons, and deletion of mouse GFR alpha -3 results in deficits in the SCG and inhibited development of intestinal Peyer’s patches (6, 7, 10, 11).

1. Wang, X. et al. (2006) Structure 14:1083.
2. Naveilhan, P. et al. (1998) Proc. Natl. Acad. Sci. USA 95:1295.
3. Nomoto, S. et al. (1998) Biochem. Biophys. Res. Commun. 244:849.
4. Baloh, R. et al. (1998) Proc. Natl. Acad. Sci. USA 95:5801.
5. Worby, C. et al. (1998) J. Biol. Chem. 273:3502.
6. Baloh, R. et al. (1998) Neuron 21:1291.
7. Honma, Y. et al. (2002) Neuron 35:267.
8. Orozco, O.E. et al.(2001) Eur. J. Neurosci. 13:2177.
9. Schlee, S. et al. (2006) Nat. Chem. Biol. 2:636.
10. Nishino, J et al. (1999) Neuron 23:725.
11. Veiga-Fernandez, H. et al. (2007) Nature 446:547.

Publications for GFR alpha-3/GDNF R alpha-3 (670-FR)(2)

Publications using 670-FR

• Wilson TR, Fridlyand J, Yan Y, Penuel E, Burton L, Chan E, Peng J, Lin E, Wang Y, Sosman J, Ribas A, Li J, Moffat J, Sutherlin DP, Koeppen H, Merchant M, Neve R, Settleman J Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors. Nature, 2012-07-26;487(7408):505-9. 2012-07-26 [PMID: 22763448] (Bioassay, Human)
• Lu B, PereiraPerrin M A novel immunoprecipitation strategy identifies a unique functional mimic of the glial cell line-derived neurotrophic factor family ligands in the pathogen Trypanosoma cruzi. Infect. Immun., 2008-06-09;76(8):3530-8. 2008-06-09 [PMID: 18541656] (PcIP, Bacteria)

Bioinformatics

• Gene Symbol: GFRA3
• Uniprot:
  • AAC24355()