Recombinant Human GFR alpha-3 Fc Chimera (CHO-expressed), CF

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

Recombinant Human GFR alpha-3 Fc Chimera (CHO-expressed), CF Summary

Details of Functionality
Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human Artemin (Catalog # 2589-AR) at 1 µg/mL can bind Recombinant Human GFR alpha ‑3/GDNF R alpha ‑3 Fc Chimera with an apparent KD < 2 nM.
Source
Chinese Hamster Ovary cell line, CHO-derived human GFR alpha-3/GDNF R alpha-3 protein
Human GFR alpha -3
(Asp32 - Asn374)
Accession # O60609
IEGRD Human IgG1
(Pro100 - Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Asp32
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
GFRA3
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
64.8 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
75-90 kDa, reducing conditions

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 400 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human GFR alpha-3 Fc Chimera (CHO-expressed), CF

  • GDNF family receptor alpha 3
  • GDNF family receptor alpha-3
  • GDNF R alpha-3
  • GDNF receptor alpha-3
  • GDNFR-alpha-3
  • GFR alpha3
  • GFR alpha-3
  • GFRA3
  • GFRa-3
  • GFR-alpha-3
  • glial cell line-derived neurotrophic factor receptor alpha-3
  • GPI-linked receptor

Background

Human glial cell line‑derived neurotrophic factor (GDNF) family receptor alpha 3 (GFR alpha ‑3, GDNF R‑ alpha 3) is an approximately 50 kDa plasma membrane glycoprotein that is one of four GDNF receptors, all of which are glycosylphosphoinositol (GPI)‑anchored, contain three conserved cysteine repeats, and promote survival of neurons (1 ‑ 4). It is synthesized as a 400 aa precursor with a 31 aa N‑terminal signal sequence, a 343 aa mature segment, and a C‑terminal GPI attachment signal sequence (3 ‑ 5). Human GFR alpha ‑3 shares 81 ‑ 84% aa sequence identity with mouse, rat, equine, bovine and canine GFR alpha ‑3. GFR alpha ‑3 is expressed in the central nervous system only during the earliest stages of neurogenesis, while later it is predominantly found in developing and adult nociceptive sensory neurons (2, 4 ‑ 8). It is expressed with the shared GDNF coreceptor, the Ret receptor tyrosine kinase, in the trigeminal ganglion, pituitary gland, thymus, lung, and duodenum (2, 4). Its ligand, the GDNF family ligand artemin, is primarily expressed by immature Schwann cells in the peripheral nervous system, and vascular smooth muscle cells, directing axonal projection of sympathetic neurons (6, 7). Artemin first binds GFR alpha ‑3, which recruits Ret, forming a signaling complex that is a pentamer containing one artemin, two GFR‑ alpha 3, and two Ret molecules (1, 6, 9). Signals from this complex are required for the development and survival of the superior cervical ganglion (SCG) neurons, and deletion of mouse GFR alpha ‑3 results in deficits in the SCG and inhibited development of intestinal Peyer’s patches (6, 7, 10, 11).

  1. Wang, X. et al. (2006) Structure 14:1083.
  2. Naveilhan, P. et al. (1998) Proc. Natl. Acad. Sci. USA 95:1295.
  3. Nomoto, S. et al. (1998) Biochem. Biophys. Res. Commun. 244:849.
  4. Baloh, R. et al. (1998) Proc. Natl. Acad. Sci. USA 95:5801.
  5. Worby, C. et al. (1998) J. Biol. Chem. 273:3502.
  6. Baloh, R. et al. (1998) Neuron 21:1291.
  7. Honma, Y. et al. (2002) Neuron 35:267.
  8. Orozco, O.E. et al.(2001) Eur. J. Neurosci. 13:2177.
  9. Schlee, S. et al. (2006) Nat. Chem. Biol. 2:636.
  10. Nishino, J et al. (1999) Neuron 23:725.
  11. Veiga-Fernandez, H. et al. (2007) Nature 446:547.

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Bioinformatics

Gene Symbol GFRA3
Uniprot