Recombinant Human Ficolin-1 Protein, CF

• Reactivity: Hu
• Applications: Binding Activity
• Format: Carrier-Free

Recombinant Human Ficolin-1 Protein, CF Summary

• Details of Functionality: Measured by its ability to bind immobilized Fetuin in a functional ELISA. Liu, Y. et al. (2005) J. Immunol. 175:3150.
• Source: Mouse myeloma cell line, NS0-derived human Ficolin-1 protein
  Gln28-Ala326, with a C-terminal 10-His tag
• Accession #: O00602
• N-terminal Sequence: Ala30
• Protein/Peptide Type: Recombinant Proteins
• Gene: FCN1
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Binding Activity
• Theoretical MW: 33.4 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 35-36 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS and NaCl.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Ficolin-1 Protein, CF

• Collagen/fibrinogen domain-containing protein 1
• FCN1
• Fcna
• FCNM
• FCNMficolin-A
• ficolin (collagen/fibrinogen domain containing) 1
• ficolin (collagen/fibrinogen domain-containing) 1
• Ficolin1
• Ficolin-1
• Ficolin-A
• ficolin-alpha
• M-ficolin

Background

Human Ficolin-1 (fibrinogen/collagen-like), also called M-ficolin, is a member of the ficolin family of secreted pattern recognition proteins in the lectin complement activation pathway (1 - 3). Ficolin-1 is expressed by monocytes, neutrophils and type II alveolar epithelial cells (2 - 5). It is proposed to be a locally-acting lectin released in a regulated manner (4). Ficolin-1 is not found in plasma, but is detected on the surface of circulating monocytes (4 - 6). The 35 kDa, 326 amino acid (aa) human Ficolin-1 contains a 28 aa signal sequence, an N-terminal collagen domain and a C-terminal fibrinogen-like (FBG) domain that includes a calcium binding site and one potential N-glycosylation site. Both the collagen and FBG domains mediate trimer formation (7). Like Ficolin-2, larger homo-multimers of Ficolin-1 exist and are likely formed by disulfide bonds at the N-terminus. Sizes corresponding to 12 or 18 subunit oligomers are reported (4, 6, 7). The FBG domain of Ficolin-1 binds microbial ligands that contain acetylated compounds (6). Ligands identified include N-acetyl glucosamine, N-acetyl galactosamine and sialyl-N-acetyllactosamine (4 - 7). Like other ficolins, Ficolin-1 associates with, and activates the MBL-associated serine protease (MASP) complex, which activates the complement pathway by cleaving C4, contributing to the innate immune response (4 - 6). Mature human Ficolin-1 shares 76%, 61%, 61%, 76% and 81% aa identity with mouse Ficolin-2 (8), and mouse, rat, canine and porcine Ficolin-1, respectively. It shares 84% and 46% aa identity with human Ficolin-2 and Ficolin-3, respectively. The Ficolin-1 gene is polymorphic, showing at least ten single nucleotide polymorphisms in the promoter and coding regions (1).

1. Endo, Y. et al. (2006) Adv. Exp. Med. Biol. 586:265.
2. Endo, Y. et al. (1996) Genomics 36:515.
3. Lu, J. et al. (1996) Biochem. J. 313: 473.
4. Liu, Y. et al. (2005) J. Immunol. 175:3150.
5. Teh, C. et al. (2000) Immunology 101:225.
6. Frederiksen, P. D. et al. (2005) Scand. J. Immunol. 62:462.
7. Tanio, M. et al. (2007) J. Biol. Chem. 282:3889.
8. Endo, Y. et al. (2004) Genomics 84:737.

Publications for Ficolin-1 (4209-FC)(1)

Publication using 4209-FC

• Dellière, S;Chauvin, C;Wong, SSW;Gressler, M;Possetti, V;Parente, R;Fontaine, T;Krüger, T;Kniemeyer, O;Bayry, J;Carvalho, A;Brakhage, AA;Inforzato, A;Latgé, JP;Aimanianda, V; Interplay between host humoral pattern recognition molecules controls undue immune responses against Aspergillus fumigatus Nature communications 2024-08-14 [PMID: 39138196] (ELISA Capture, Fungus - Aspergillus fumigatus)

Bioinformatics

• Gene Symbol: FCN1
• Uniprot:
  • O00602()