Recombinant Human FABP2/I-FABP Protein, CF

• Reactivity: Hu
• Format: Carrier-Free

Recombinant Human FABP2/I-FABP Protein, CF Summary

• Source: E. coli-derived human FABP2/I-FABP protein
  Ala2-Asp132, with a C-terminal 6-His tag
• Accession #: P12104
• N-terminal Sequence: Ala2
• Protein/Peptide Type: Recombinant Proteins
• Gene: FABP2
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Theoretical MW: 16 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 15 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
• Buffer: Supplied as a 0.2 μm filtered solution in PBS and Glycerol.
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human FABP2/I-FABP Protein, CF

• FABP2
• FABPI
• fatty acid binding protein 2, intestinal
• Fatty acid-binding protein 2
• fatty acid-binding protein, intestinal
• IFABP
• I-FABP
• I-FABPMGC133132
• Intestinal FABP
• Intestinal-type fatty acid-binding protein

Background

Fatty acid binding protein-2 (FABP-2; also named I- or intestinal FABP) is a member of a large superfamily of lipid binding proteins that are expressed in a tissue specific manner (1-3). FABP-2 is one of nine cytoplasmic FABPs that are 14-15 kDa in size and range from 126-134 amino acids (aa) in length (2). Although all are highly conserved in their tertiary structure, there is only modest aa identity between any two members. Nevertheless, based on aa sequence, the nine FABP family members have been shown to form three subgroups, with FABP‑2/I‑FABP linked with liver/L-FABP and heart/H-FABP (2). The designation of a tissue type, such as intestinal, does not suggest the binding protein is universally expressed in all cell types that make up the organ or tissue. Human I-FABP, the product of the FABP-2 gene, is a 132 aa cytosolic protein that shows a flattened beta -barrel structure (called a beta -clam) generated by a series of antiparallel beta -strands and two alpha ‑helices (1, 2, 4). Preferred ligands for FABP-2 include sixteen to twenty carbon long chain fatty acids (4). It is suggested that ligands first bind to the outside of the molecule, and this binding subsequently induces a conformational change in the binding protein, resulting in "internalization" of the ligand (1). An Ala-to-Thr polymorphism at position #54 has been reported to potentially impact FABP-2 function (2). This polymorphism has been suggested to be associated with an increased risk of type II diabetes. To date, the evidence appears to be equivocal (1, 2). This polymorphism may, however, have unusual metabolic effects depending upon the type of diet involved (1, 5). Human FABP-2 is 78%, 82% and 86% aa identical to mouse, rat and canine FABP-2, respectively. It also shows 33% and 24% aa identity to human H-FABP and L‑FABP, respectively. FABP-2 is proposed to transport fatty acids (FA) into cells, increase FA availability to enzymes, protect cell structures from FA attack, and target FA to transcription factors in the nuclear lumen (3). The binding affinity of Recombinant Human FABP2/I FABP (Catalog # 2694-CL/CF) for the synthetic ligand cis-parinaric acid had historically been measured at R&D Systems by fluorescence titration (6), but cis-parinaric acid is no longer commercially available and testing is discontinued. Historically, half-maximal fluorescence of 3 μM Recombinant Human FABP2/I FABP was achieved with approximately 3 μM cis-paranaric acid.

1. Weiss, E.P. et al. (2002) Physiol. Genomics 10:145.
2. Zimmerman, A.W. and J.H. Veerkamp (2002) Cell. Mol. Life Sci. 59:1096.
3. Haunerland, N.H. and F. Spener (2004) Prog. Lipid Res. 43:328.
4. Sweetser, D.A. et al. (1987) J. Biol. Chem. 262:16060.
5. Dworatzek, P. et al. (2004) Am. J. Clin. Nutr. 79:1110.
6. Nemecz, G. et al. (1991) Arch. Biochem. Biophys. 286:300.

Publications for FABP2/I-FABP (2694-CL/CF)(1)

Publication using 2694-CL/CF

• Stejskal D, Karpisek M Adipocyte fatty acid binding protein in a Caucasian population: a new marker of metabolic syndrome? Eur. J. Clin. Invest., 2006-09-01;36(9):621-5. 2006-09-01 [PMID: 16919044] (ELISA (Specificity Testing), Human)

Bioinformatics

• Gene Symbol: FABP2
• Uniprot:
  • P12104()