Recombinant Human F-Spondin/SPON1 Protein

• Reactivity: Hu
• Applications: Bioactivity

Recombinant Human F-Spondin/SPON1 Protein Summary

• Details of Functionality: Measured by its ability to enhance neurite outgrowth of dissociated E13 chick embryonic dorsal root ganglia (DRG) neurons. Able to significantly enhance neurite outgrowth when immobilized as a 3 µL droplet containing 100 ng on a nitrocellulose-coated microplate.
• Source: Mouse myeloma cell line, NS0-derived human F-Spondin/SPON1 protein
  Phe29-Cys807, with a C-terminal 6-His tag
• Accession #: Q9HCB6
• N-terminal Sequence: Phe29
• Protein/Peptide Type: Recombinant Proteins
• Gene: SPON1
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 88.9 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 110-115 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human F-Spondin/SPON1 Protein

• FSpondin
• F-Spondin
• KIAA0762VSGP/F-spondin
• MGC10724
• SPON1
• spondin 1, (f-spondin) extracellular matrix protein
• spondin 1, extracellular matrix protein
• spondin-1
• Vascular smooth muscle cell growth-promoting factor
• VSGP

Background

F-Spondin (floor plate and thrombospondin homology), also called Spondin-1, SPON1 or VSGP (vascular smooth muscle growth-promoting factor), is an approximately 110 kDa secreted glycoprotein that is a member of a subgroup of TSR (thrombospondin) molecules that are either membrane-bound or associated with the extracellular matrix (ECM) (1-3). Human F-Spondin is synthesized as an 807 amino acid (aa) precursor with a 779 aa mature region that includes an N-terminal reelin‑like domain, an F-spondin (FS) domain, and six C‑terminal thrombospondin (TSP) type I repeats (1-3). Mature human F-Spondin shares 97%, 97%, 98% and 99% aa sequence identity with mouse, rat, bovine and canine F-Spondin, respectively.  TSP 5 and 6 bind ECM, while TSP 1-4 plus the FS domain may mediate repulsive activity on motor neurons and outgrowth promoting activity on sensory neurons during development or after injury (2-5). Crystal structure indicates that the reelin-like domain binds heparin and may mediate weak dimerization (6). Plasmin cleavage generates a diffusible 95 kDa, 656 aa F-spondin that lacks TSP 5 and 6, while non‑plasmin cleavage between the FS segment and the first TSP repeat generates 60 kDa and 50 kDa fragments (3, 4, 7). F-Spondin shows unusual C‑mannosylation and O-fucosylation within the TSP repeats (3). Mammalian cells expressing F-spondin include floor plate epithelium, ventral motor neurons, Schwann cells, fibroblasts, hippocampal pyramidal cells, endothelial cells, vascular smooth muscle cells and some tumor cells (2-5, 8). F-Spondin can either tether cells to the ECM or interfere with integrin adhesion, thus either blocking or allowing nerve or vascular endothelial cell migration (3, 9). It binds beta -amyloid fibrils and inhibits beta ‑secretase cleavage, thus reducing A beta plaque deposition associated with Alzheimer’s disease (10, 11). F-Spondin is also reported to inhibit differentiation or migration during angiogenesis (affecting endothelial cells) and bone development (affecting osteoclast and chondrocyte precursors) (3, 9, 12, 13).

1. Miyamoto, K. et al. (2001) Arch. Biochem. Biophys. 390:93.
2. Klar, A. et al. (1992) Cell 69:95.
3. Feinstein, Y. and A. Klar (2004) Int. J. Biochem. Cell Biol. 36:975.
4. Burstyn-Cohen, T. et al. (1998) J. Neurosci. 18:8875.
5. Feinstein, Y. et al. (1999) Development 126:3637.
6. Tan, K. et al. (2008) J. Mol. Biol. 381:1213.
7. Tzarfaty-Majar, V. et al. (2001) J. Biol. Chem. 276:28233.
8. Pyle-Chenault, R.A. et al. (2005) Tumor Biol. 26:245.
9. Terai, Y. et al. (2001) J. Cell Physiol. 188:394.
10. Ho, A. and T.C. Sudhof (2004) Proc. Natl. Acad. Sci. USA 101:2548.
11. Hafez, D.M. et al. (2012) Neuroscience 223:465.
12. Palmer, G.D. et al. (2010) J. Orthop. Res. 28:1323.
13. Oka, H. et al. (2011) J. Periodontol. 82:1776.

Publications for F-Spondin/SPON1 (3135-SP)(2)

Publications using 3135-SP

• J Song, SL Merbs, LJ Sokoll, DW Chan, Z Zhang A multiplex immunoassay of serum biomarkers for the detection of uveal melanoma Clin Proteomics, 2019-03-05;16(0):10. 2019-03-05 [PMID: 30867659] (Human)
• Cheng YC, Liang CM, Chen YP, Tsai IH, Kuo CC, Liang SM F-spondin plays a critical role in murine neuroblastoma survival by maintaining IL-6 expression. J. Neurochem., 2009-06-22;110(3):947-55. 2009-06-22 [PMID: 19549008] (Western Blot)

Bioinformatics

• Gene Symbol: SPON1
• Uniprot:
  • Q9HCB6()