Recombinant Human Ephrin-B2 Fc Chimera Protein, CF


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Product Details

Reactivity HuSpecies Glossary
Applications Bioactivity

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Recombinant Human Ephrin-B2 Fc Chimera Protein, CF Summary

Details of Functionality
Measured by its binding ability in a functional ELISA. When Recombinant Human EphB2 Fc Chimera (Catalog # 5189-B2) is coated at 2 μg/mL, Recombinant Human Ephrin‑B2 Fc Chimera binds with an apparent Kd <0.1 nM.
Also measured by its ability to enhance neurite outgrowth of E16-E18 rat embryonic cortical neuron.
Mouse myeloma cell line, NS0-derived human Ephrin-B2 protein
Human Ephrin-B2
Accession # P52799
N-terminus C-terminus
Accession #
N-terminal Sequence
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.


Theoretical MW
48.8 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
58-66 kDa, reducing conditions
Read Publications using
7397-EB in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS.
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 300 μg/mL in PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Ephrin-B2 Fc Chimera Protein, CF

  • EFNB2
  • ELF-2
  • ephrin B2
  • EphrinB2
  • Ephrin-B2
  • EPLG5
  • HTKL
  • Htk-L
  • LERK5
  • LERK-5
  • NLERK-1


Ephrin-B2, also known as Htk-L, ELF-2, LERK-5, and NLERK-1, is a 40 kDa member of the Ephrin-B family of transmembrane ligands that bind and induce the tyrosine autophosphorylation of Eph receptors. The extracellular domains of Ephrin-B ligands are structurally related to GPI-anchored Ephrin-A ligands. Eph-Ephrin interactions are widely involved in the regulation of cell migration, tissue morphogenesis, and cancer progression. Ephrin-B2 preferentially interacts with receptors in the EphB family (1, 2). Mature human Ephrin-B2 consists of a 202 amino acid (aa) extracelluar domain (ECD), a 21 aa transmembrane segment, and an 83 aa cytoplasmic domain (3). Within the ECD, human Ephrin-B2 shares 97% aa sequence identity with mouse and rat Ephrin-B2. Ephrin-B2 is expressed presynaptically on neurons (4, 5). It promotes presynaptic development, EphB2 shedding, axonal growth cone collapse, and neurite repulsion, and also regulates inflammatory and neuropathic pain (4-6). Ephrin-B2 is expressed by vascular mural cells and arterial vascular and lymphatic endothelium (7, 8). It exerts proliferative and migratory effects on these cells during angiogenesis and lymphangiogenesis in part by regulating the signaling activity of VEGF R2 and VEGF R3 (7-9). Ephrin-B2 plays a role in the immune response by mediating monocyte extravasation and T cell costimulation (10, 11). It is up‑regulated in invasive cancers and promotes tumor cell migration, invasion, and tumor angiogenesis (12-14). It functions as a cellular entry receptor for Hendra and Nipah viruses (15). Ephrin-B2 is also important for the separation of the urinary and intestinal tracts during development (16).
  1. Miao, H. and B. Wang (2009) Int. J. Biochem. Cell Biol. 41:762.
  2. Pasquale, E.B. (2010) Nat. Rev. Cancer 10:165.
  3. Cerretti, D.P. et al. (1995) Mol. Immunol. 32:1197.
  4. McClelland, A.C. et al. (2009) Proc. Natl. Acad. Sci. 106:20487.
  5. Zhao, J. et al. (2010) Mol. Pain 6:77.
  6. Lin, K.-T. et al. (2008) J. Biol. Chem. 283:28969.
  7. Foo, S.S. et al. (2006) Cell 124:161.
  8. Wang, Y. et al. (2010) Nature 465:483.
  9. Sawamiphak, S. et al. (2010) Nature 465:487.
  10. Pfaff, D. et al. (2008) J. Cell Sci. 121:3842.
  11. Yu, G. et al. (2003) J. Immunol. 171:106.
  12. Meyer, S. et al. (2005) Int. J. Oncol. 27:1197.
  13. Nakada, M. et al. (2010) Int. J. Cancer 126:1155.
  14. Liu, W. et al. (2004) Br. J. Cancer 90:1620.
  15. Bonaparte, M.I. et al. (2005) Proc. Natl. Acad. Sci. 102:10652.
  16. Dravis, C. et al. (2004) Dev. Biol. 271:272.

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Publications for Ephrin-B2 (7397-EB)(9)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: Bioassay.

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Showing Publications 1 - 9 of 9.
Publications using 7397-EB Applications Species
R Hilal, M Poittevin, A Pasteur-Ro, A Cogo, G Mangin, M Chevauché, Y Ziat, J Vilar, JM Launay, JF Gautier, D Broquères-, BI Levy, T Merkulova-, N Kubis Diabetic Ephrin-B2-Stimulated Peripheral Blood Mononuclear Cells Enhance Poststroke Recovery in Mice Stem Cells Int, 2018;2018(0):2431567. 2018 [PMID: 29736174] (Bioassay, Human) Bioassay Human
C You, K Zhao, P Dammann, K Keyvani, I Kreitschma, U Sure, Y Zhu EphB4 forward signalling mediates angiogenesis caused by CCM3/PDCD10-ablation J. Cell. Mol. Med., 2017;0(0):. 2017 [PMID: 28371279] (Bioassay, Human) Bioassay Human
Downregulation of proinflammatory cytokines in HTLV-1-infected T cells by Resveratrol J Exp Clin Cancer Res, 2016;35(1):118. 2016 [PMID: 27448598] (Bioassay, Human) Bioassay Human
EPHB4 kinase-inactivating mutations cause autosomal dominant lymphatic-related hydrops fetalis J Clin Invest, 2016;0(0):. 2016 [PMID: 27400125] (Bioassay, Human) Bioassay Human
Lisle J, Mertens-Walker I, Stephens C, Stansfield S, Clements J, Herington A, Stephenson S Murine, but not human, ephrin-B2 can be efficiently cleaved by the serine protease kallikrein-4: implications for xenograft models of human prostate cancer. Exp Cell Res, 2015;333(1):136-46. 2015 [PMID: 25724897] (Bioassay, Human) Bioassay Human
Kawasaki, Jun, Aegerter, Sandrine, Fevurly, R Dawn, Mammoto, Akiko, Mammoto, Tadanori, Sahin, Mustafa, Mably, John D, Fishman, Steven J, Chan, Joanne RASA1 functions in EPHB4 signaling pathway to suppress endothelial mTORC1 activity. J Clin Invest, 2014;124(6):2774-84. 2014 [PMID: 24837431] (Bioassay, Human) Bioassay Human
Grafton K, Moir L, Black J, Hansbro N, Hansbro P, Burgess J, Oliver B LF-15 &amp; T7, synthetic peptides derived from tumstatin, attenuate aspects of airway remodelling in a murine model of chronic OVA-induced allergic airway disease. PLoS ONE, 2014;9(1):e85655. 2014 [PMID: 24454912] (Bioassay, Human) Bioassay Human
Liu, Qian, Stone, Jacquely, Bradel-Tretheway, Birgit, Dabundo, Jeffrey, Benavides Montano, Javier A, Santos-Montanez, Jennifer, Biering, Scott B, Nicola, Anthony, Iorio, Ronald M, Lu, Xiaonan, Aguilar, Hector C Unraveling a three-step spatiotemporal mechanism of triggering of receptor-induced Nipah virus fusion and cell entry. PLoS Pathog, 2013;9(11):e1003770. 2013 [PMID: 24278018] (Bioassay, Human) Bioassay Human
Kida Y, Ieronimakis N, Schrimpf C, Reyes M, Duffield J EphrinB2 reverse signaling protects against capillary rarefaction and fibrosis after kidney injury. J Am Soc Nephrol, 2015;24(4):559-72. 2015 [PMID: 23492730] (Bioassay, Human) Bioassay Human

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Gene Symbol EFNB2