Recombinant Human EphB3 Fc Chimera Protein, CF

• Reactivity: Hu
• Applications: Binding Activity
• Format: Carrier-Free

Recombinant Human EphB3 Fc Chimera Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human EphB3 Fc Chimera at 2 μg/mL (100 μL/well) can bind recombinant mouse Ephrin-B1 Fc Chimera with a linear range of 0.04-2.5 ng/mL
• Source: Mouse myeloma cell line, NS0-derived human EphB3 protein
  Leu38-Ala550
• Accession #: P54753
• N-terminal Sequence: Leu38
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Gene: EPHB3
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Endotoxin Note: <0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Binding Activity
• Theoretical MW: 82.7 kDa (monomer).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 90-100 kDa under reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution Instructions: Reconstitute at 200 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human EphB3 Fc Chimera Protein, CF

• Cek10
• EC 2.7.10
• EC 2.7.10.1
• EK2
• EPH receptor B3
• EphB3
• EPH-like kinase 2
• EPH-like tyrosine kinase-2
• ephrin type-B receptor 3
• ETK2Hek2
• Hek2
• human embryo kinase 2
• Mdk5
• Sek4
• Tyro6
• Tyrosine-protein kinase TYRO6

Background

EphB3, also known as Cek10, Tyro6, Sek4, Hek2, and Mdk5, is a 130 kDa member of the transmembrane Eph receptor tyrosine kinase family. The A and B classes of Eph proteins are distinguished by Ephrin ligand binding preference but have a common structural organization. Eph-Ephrin interactions are widely involved in the regulation of cell migration, tissue morphogenesis, and cancer progression (1). The 526 amino acid (aa) extracellular domain (ECD) of mature human EphB3 contains a ligand binding domain followed by a cysteine rich region and two fibronectin type III domains. The 418 aa cytoplasmic domain contains a tyrosine kinase domain, a sterile alpha motif (SAM), and a PDZ binding motif (2). Within the ECD, human EphB3 shares 96% aa sequence identity with mouse and rat EphB3. Binding of EphB3 to its ligands Ephrin-B1, B2, and B3 triggers forward signaling through EphB3 as well as reverse signaling through the Ephrin (1, 3). EphB3 also interacts in cis with the receptor tyrosine kinase Ryk (4). Activation of its kinase is required for some but not all of the effects of EphB3 on cellular adhesion, motility, and morphology (5). EphB3 is widely expressed during development and in the adult; it shows a complementary tissue distribution to the Ephrin-B ligands (6-9). EphB3 function is important in vascular, nervous system, thymocyte, and palate development (6, 7, 10-12). It directs embyronic neuronal axon pathfinding, and its up-regulation on local macrophages following neuronal injury promotes the growth of regenerating axons (10, 13). EphB3 inhibits colorectal carcinogenesis and invasion by preventing the migration of tumor cells out of the intestinal crypt (9, 14). In non-small cell lung cancer, however, it is up-regulated and can promote tumor progression (15). EphB3 function is supported by the cooperative action of EphB2 in several of these processes (6, 10-12, 16).

1. Park, I. and H.S. Lee (2015) Mol. Cells 38:14.
2. Bohme, B. et al. (1993) Oncogene 8:2857.
3. Pasquale, E.B (2004) Nat. Neurosci. 7:417.
4. Trivier, E. and T.S. Ganesan (2002) J. Biol. Chem. 277:23037.
5. Miao, H. et al. (2005) J. Biol. Chem. 280:923.
6. Adams, R.H. et al. (1999) Genes Dev. 13:295.
7. Krull, C.E. et al. (1997) Curr. Biol. 7:571.
8. Willson, C.A. et al. (2006) J. Mol. Histol. 37:369.
9. Cortina, C. et al. (2007) Nature Genet. 39:1376.
10. Birgbauer, E. et al. (2000) Development 127:1231.
11. Alfaro, D. et al. (2008) Immunology 125:131.
12. Risley, M. et al. (2009) Mech. Dev. 126:230.
13. Liu, X. et al. (2006) J. Neurosci. 26:3087.
14. Batlle, E. et al. (2005) Nature 435:1126.
15. Ji, X.-D. et al. (2011) Cancer Res. 71:1156.
16. Holmberg, J. et al. (2006) Cell 125:1151.

Publications for EphB3 (5667-B3)(4)

Publications using 5667-B3

• Larsen, BB;McMahon, T;Brown, JT;Wang, Z;Radford, CE;Crowe, JE;Veesler, D;Bloom, JD; Functional and antigenic landscape of the Nipah virus receptor binding protein bioRxiv : the preprint server for biology 2024-04-19 [PMID: 38659959] (Immunocytochemistry, Chinese Hamster)
• AK Gro beta kopf, S Schlagowsk, T Fricke, A Ensser, RC Desrosiers, AS Hahn Plxdc family members are novel receptors for the rhesus monkey rhadinovirus (RRV) PloS Pathogens, 2021-03-03;17(3):e1008979. 2021-03-03 [PMID: 33657166] (Bioassay, Human)
• Zhang , Gu, Brady , John, Liang , Wei-Chin, Wu , Yan, Henkemeyer , Mark, Yan , Minhong EphB4 forward signalling regulates lymphatic valve development. Nat Commun, 2015-04-13;6(0):6625. 2015-04-13 [PMID: 25865237] (Enzyme Assay, Human)
• Aguilar HC, Aspericueta V, Robinson LR A quantitative and kinetic fusion protein-triggering assay can discern distinct steps in the nipah virus membrane fusion cascade. J. Virol., 2010-06-02;84(16):8033-41. 2010-06-02 [PMID: 20519383] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: EPHB3
• Uniprot:
  • P54753(Human)