Recombinant Human EphA10 Fc Chimera Protein, CF

• Reactivity: Hu
• Applications: Binding Activity
• Format: Carrier-Free

Recombinant Human EphA10 Fc Chimera Protein, CF Summary

• Details of Functionality: Measured by its ability to bind biotinylated rhEphrin-A3/Fc Chimera in a functional ELISA.
• Source: Mouse myeloma cell line, NS0-derived human EphA10 protein
  

  Human EphA10 (Glu34-Ala565) Accession # NP_001092909	IEGRMD	Human IgG1 (Pro100-Lys330)
  N-terminus		C-terminus

• Accession #: NP_001092909
• N-terminal Sequence: Glu34
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Gene: EPHA10
• Purity: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Binding Activity
• Theoretical MW: 84.3 kDa (monomer).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 100-106 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 200 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human EphA10 Fc Chimera Protein, CF

• EC 2.7.10.1
• EPH receptor A10
• EphA10
• EphA10s protein
• ephrin type-A receptor 10
• FLJ16103
• FLJ33655
• MGC43817

Background

EphA10 is a member of the transmembrane Eph receptor tyrosine kinase family. Although the A and B classes of Eph proteins have a common structural organization, they are distinguished by their preference for Ephrin ligands. Eph-Ephrin interactions are widely involved in the regulation of cell migration, tissue morphogenesis, and cancer progression (1-4). The 532 amino acid (aa) extracellular domain (ECD) of human EphA10 contains an N-terminal Ephrin binding region followed by two fibronectin type II domains, a 422 aa cytoplasmic domain contains the tyrosine kinase domain and a sterile alpha motif (SAM) (5). Alternate splicing generates a soluble isoform that is truncated following the Ephrin binding region, and a transmembrane isoform that lacks only the SAM (5). Within the ECD, human EphA10 shares 38%-54% aa sequence identity with other EphA and EphB proteins. It shares 95% and 88% aa sequence identity with the ECD of mouse and rat EphA10, respectively. EphA10 is preferentially expressed in testis and is upregulated in some breast and prostate carcinoma cell lines (5- 7). It selectivity binds Ephrin-A1, 2, 3, 4,and 5 relative to Ephrin-B1 and -B2 (5).

1. Pasquale, E.B. (2008) Cell 133:38.
2. Merlos-Suarez, A. and E. Batlle (2008) Curr. Opin. Cell Biol. 20:194.
3. Arvanitis, D. and A. Davy (2008) Genes Dev. 22:416.
4. Robinson, D.R. et al. (2000) Oncogene 19:5548.
5. Aasheim, H.-C. et al. (2005) Biochim. Biophys. Acta 1723:1.
6. Fox, B.P. et al. (2006) Biochem. Biophys. Res. Commun. 342:1263.
7. Fox, B.P. and R.P. Kandpal (2004) Biochem. Biophys. Res. Commun. 318:882.

Publications for EphA10 (5188-EA)(1)

Publication using 5188-EA

• JH Cha, LC Chan, YN Wang, YY Chu, CH Wang, HH Lee, W Xia, WC Shyu, SP Liu, J Yao, CW Chang, FR Cheng, J Liu, SO Lim, JL Hsu, WH Yang, GN Hortobagyi, C Lin, L Yang, D Yu, LB Jeng, MC Hung Ephrin receptor A10 monoclonal antibodies and the derived chimeric antigen receptor T cells exert an antitumor response in mouse models of triple-negative breast cancer The Journal of Biological Chemistry, 2022-03-10;0(0):101817. 2022-03-10 [PMID: 35278434] (Bioassay, ELISA Capture, Human, N/A)

Bioinformatics

• Gene Symbol: EPHA10
• Uniprot:
  • NP_001092909()