Recombinant Human EpCAM/TROP1 His-tag Protein, CF

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human EpCAM/TROP1 His-tag Protein, CF Summary

• Details of Functionality: Measured by the ability of the immobilized protein to support the adhesion of the L Cells mouse fibroblast cell line. When 5 x 10⁴ cells/well are added to Recombinant Human EpCAM/TROP-1 and Human Fibronectin (0.5 μg/mL, Catalog # 1918-FN) coated plates, cell adhesion is enhanced in a dose dependent manner. The ED₅₀ for this effect is 0.4-2.4 μg/mL.
• Source: Human embryonic kidney cell, HEK293-derived human EpCAM/TROP1 protein
  Gln24-Lys265, with a C-terminal 6-His tag
• Accession #: P16422
• N-terminal Sequence: No results obtained. Gln24 inferred from enzymatic pyroglutamate treatment revealing Glu25
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Theoretical MW: 28 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 30-42 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human EpCAM/TROP1 His-tag Protein, CF

• 17-1A
• 323/A3
• ACSTD1
• antigen identified by monoclonal AUA1
• CD326 antigen
• CD326
• Cell surface glycoprotein Trop-1
• chromosome 4, surface marker (35kD glycoprotein)
• DIAR5
• EGP
• EGP-2
• EGP314
• EGP40
• EpCAM
• epithelial cell adhesion molecule
• Epithelial cell surface antigen
• Epithelial glycoprotein 314
• Epithelial glycoprotein
• ESA
• GA733-2
• GA733-2EGP34
• gp40
• hEGP314
• HNPCC8
• KS 1/4 antigen
• KS1/4
• KSAHEA125
• M1S2
• M4S1
• M4S1Ly74
• Major gastrointestinal tumor-associated protein GA733-2
• MIC18MH99
• MOC31
• TACST-1
• TACSTD1
• TROP1
• TROP1CD326
• Tumor-associated calcium signal transducer 1CO-17A

Background

Epithelial Cellular Adhesion Molecule (EpCAM), also known as KS1/4, gp40, GA733-2, 17-1A, and TROP‑1, is a 40 kDa transmembrane glycoprotein that consists of a 242 amino acid (aa) extracellular domain with two EGF‑like repeats, a 23 aa transmembrane segment, and a 26 aa cytoplasmic domain (1). Human and mouse EpCAM share 82% aa sequence identity. During embryonic development, EpCAM is detected in fetal lung, kidney, liver, pancreas, skin, and germ cells. In adults, human EpCAM is expressed on basolateral cell membranes of all simple, pseudo-stratified, and transitional epithelia but not on normal squamous stratified epithelia, mesenchymal tissue, muscular tissue, neuro-endocrine tissue, or lymphoid tissue (2). It is additionally expressed on undifferentiated embryonic stem cells, thymocytes, and dendritic cells (3-5). It is up-regulated on actively proliferating epithelial tissues, during adult liver regeneration, and on many epithelial cell-derived carcinomas (2, 6). EpCAM functions as a homophilic cell adhesion molecule (7). It associates into tetramers and forms complexes in cis with Claudin-7, CD44v6, TSPAN8, CD9, Integrin alpha 3, and Annexin A1 (8-11) that can interfere with cell adhesion (12, 13). Proteolytic cleavage of EpCAM releases multiple fragments from the ECD as well as a cytoplasmic fragment that can regulate gene transcription (14-16).

1. Strnad, J. et al. (1989) Cancer Res. 49:314.
2. Schnell, U. et al. (2013) Biochim. Biophys. Acta 1828:1989.
3. Ng, V.Y. et al. (2010) Stem Cells 28:29.
4. Nelson, A.J. et al. (1996) Eur. J. Immunol. 26:401.
5. Borkowski, T.A. et al. (1996) Eur. J. Immunol. 26:110.
6. de Boer, C.J. et al. (1999) J. Pathol. 188:201.
7. Litvinov, S.V. et al. (1994) J. Cell Biol. 125:437.
8. Balzar, M. et al. (2001) Mol. Cell. Biol. 21:2570.
9. Nubel, T. et al. (2009) Mol. Cancer Res. 7:285.
10. Kuhn, S. et al. (2007) Mol. Cancer Res. 5:553.
11. Schmidt, D.S. et al. (2004) Exp. Cell Res. 297:329.
12. Litvinov, S.V. et al. (1997) J. Cell Biol. 139:1337.
13. Gaiser, M.R. et al. (2012) Proc. Natl. Acad. Sci. USA 109:E889.
14. Schnell, U. et al. (2013) Biosci. Rep. 33:e00030.
15. Schon, M.P. et al. (1993) Int. J. Cancer 55:988.
16. Maetzel, D. et al. (2009) Nat. Cell Biol. 11:162.

Publications for EpCAM/TROP1 (9277-EP)(1)

Publication using 9277-EP

• I Song, Y Kang, YK Lee, SC Myung, K Ko Endoplasmic reticulum retention motif fused to recombinant anti-cancer monoclonal antibody (mAb) CO17-1A affects mAb expression and plant stress response PLoS ONE, 2018;13(9):e0198978. 2018 [PMID: 30248125] (Bioassay, Bacteria - Agrobacterium tumefaciens)

FAQs for EpCAM/TROP1 (9277-EP). (Showing 1 - 1 of 1 FAQ).

1. I'm looking for EpCAM antibody for rat. Do you have any antibody which can use FACS?
   • We unfortunately do not have any EpCAM antibodies validated in FACS for detection of the rat protein. I am sorry for any inconvenience. I do have three products to EpCAM that can detect the rat protein. These products have been validated to detect the native rat protein, so they may work for FACS however they have not yet been tested in that particular application. If you would like to try them in your experiment you would qualify for our Innovator's Reward Program. This program was designed to help scientists with the cost of their antibodies in return for valuable information. If you decide to test an antibody in a previously untested species or application we will issue you a credit for the purchase price of the antibody in exchange for a review of your experiment using our product.

Bioinformatics

• Uniprot:
  • P16422()