Recombinant Human Endoglycan/PODXL2 Protein, CF

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human Endoglycan/PODXL2 Protein, CF Summary

• Details of Functionality: Measured by the ability of the immobilized protein to inhibit the adhesion of CHO Chinese hamster ovary cells transfected with P-Selectin. The ED₅₀ for this effect is 0.1-0.6 μg/mL.
• Source: Mouse myeloma cell line, NS0-derived human Endoglycan/PODXL2 protein
  Gly33-Thr500, with a C-terminal 10-His tag
• Accession #: Q9NZ53
• N-terminal Sequence: Gly33
• Protein/Peptide Type: Recombinant Proteins
• Gene: PODXL2
• Purity: >95%, by SDS-PAGE with silver staining
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 52 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 85-110 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE with silver staining
• Reconstitution Instructions: Reconstitute at 100 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Endoglycan/PODXL2 Protein, CF

• Endoglycan
• PODLX2
• podocalyxin-like 2
• podocalyxin-like protein 2
• PODXL2

Background

Human Endoglycan, also called Podocalyxin-like 2 protein (PODXL2), is an extensively O-glycosylated type I transmembrane protein in the CD34 family of sialomucins (1, 2). Mature human Endoglycan consists of a 468 amino acid (aa) extracellular domain that contains an N-terminal acidic domain characterized by several polyglutamate tracts, a mucin domain, and a membrane-proximal globular domain. Within the extracellular domain, human Endoglycan shares 74% amino acid identity with both mouse and rat Endoglycan. Alternative splicing may generate a short isoform that lacks aa 336-411. Following the extracellular domain is a single transmembrane spanning helix and a charged cytoplasmic tail that contains a C-terminal PDZ-domain binding motif (1, 3). The cytoplasmic domain of Endoglycan has been shown to bind to Na⁺/H⁺ exchanger regulatory factor-1 (NHERF-1), which acts as a scaffolding protein that links membrane proteins to the actin cytoskeleton (4). Human Endoglycan exists as a disulfide-linked homodimer that is expressed in vascular endothelium, smooth muscle, hematopoietic precursor populations and leukocyte subpopulations (1, 5). Endoglycan functions through its interactions with selectins and is involved in leukocyte-endothelial cell adhesion although depending on cellular context and post-translational modifications, Endoglycan may also demonstrate anti-adhesive properties (3, 5).

 

1. Sassetti, C. et al. (2000) J. Biol. Chem. 275:9001.
2. Fieger, C.B. et al. (2003) J. Biol. Chem. 278:27390.
3. Nielsen, J.S. & K.M. McNagny (2008) J. Cell Sci. 121:3683.
4. Tan, P.C. et al. (2006) Stem Cells 24:1150.
5. Kerr, S.C. et al. (2008) J. Immunol. 181:1480.
 

Publications for Endoglycan/PODXL2 (1524-EG)(1)

Publication using 1524-EG

• Pruenster, M;Immler, R;Roth, J;Kuchler, T;Bromberger, T;Napoli, M;Nussbaumer, K;Rohwedder, I;Wackerbarth, LM;Piantoni, C;Hennis, K;Fink, D;Kallabis, S;Schroll, T;Masgrau-Alsina, S;Budke, A;Liu, W;Vestweber, D;Wahl-Schott, C;Roth, J;Meissner, F;Moser, M;Vogl, T;Hornung, V;Broz, P;Sperandio, M; E-selectin-mediated rapid NLRP3 inflammasome activation regulates S100A8/S100A9 release from neutrophils via transient gasdermin D pore formation Nature immunology 2023-10-30 [PMID: 37903858] (Bioassay, Human)

Bioinformatics

• Gene Symbol: PODXL2
• Uniprot:
  • Q9NZ53()